| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,195,481 | A→G | 24.4% | C557C (TGT→TGC) | yoaA ← | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,195,481 | 0 | A | G | 24.4% | 127.7 / 37.7 | 106 | C557C (TGT→TGC) | yoaA | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| Reads supporting (aligned to +/- strand): ref base A (43/37); new base G (13/13); total (56/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.23e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-01 | |||||||||||
Only 100 of 106 total aligned reads displayed.
TGAGAGTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTTGTCGAT > minE/1195420‑1195543 | tGAGAGTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCt > 1:369364/1‑68 (MQ=255)tGAGAGTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCt > 1:1109759/1‑68 (MQ=255)tGAGAGTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCt > 1:3343862/1‑68 (MQ=255)tGAGAGTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCt > 1:3281235/1‑68 (MQ=255)tGAGAGTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCt > 1:2753097/1‑68 (MQ=255)tGAGAGTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCt > 1:2606907/1‑68 (MQ=255)tGAGAGTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCt > 1:2279381/1‑68 (MQ=255)tGAGAGTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCt > 1:2046670/1‑68 (MQ=255)tGAGAGTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCt > 1:1309215/1‑68 (MQ=255)tGAGAGTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCt > 1:1572431/1‑68 (MQ=255)tGAGAGTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCt > 1:1388854/1‑68 (MQ=255) agagTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGTTCGCCACCGCGCAAACGACAATCTTc < 1:1027920/68‑1 (MQ=255) agagTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTc < 1:1205203/68‑1 (MQ=255) agagTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTc < 1:1737149/68‑1 (MQ=255) agagTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTc < 1:3168337/68‑1 (MQ=255) agagTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTc < 1:3299977/68‑1 (MQ=255) agagTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTc < 1:1497078/68‑1 (MQ=255) gACGGCATCTGGTAGTTGCACTTCATCTAACGGGTCGCCACCGCGCAAACGACAATCTTCCATgcgcg < 1:1240699/68‑1 (MQ=255) gACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATgcgcg < 1:592706/68‑1 (MQ=255) gACGGCATCTGGTAGTTGCACTTCATCGAACGGGGCGCCACCGCGCAAACGACAATCTTc < 1:1649760/60‑1 (MQ=255) gCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCtttt < 1:2651409/69‑1 (MQ=255) gCATCCGGCAGTTGTACTTCATCGAACGGATCACCACCGCGCAAACGGCAATCTTcc > 1:2750770/1‑57 (MQ=255) gCATCCGGCAGTTGTACTTCATCGAACGGATCACCACCGCGCAAACGGCAATCTTCCATGCGTGCtttt > 1:371337/1‑69 (MQ=255) gCATCCGGCAGTTGTACTTCATCGAACGGATCACCACCGCGCAAACGGCAATCTTCCATGCGTGCtttt > 1:369199/1‑69 (MQ=255) gCATCCGGCAGTTGTACTTCATCGAACGGATCACCACCGCGCAAACGGCAATCTTCCATGCGTGCtttt > 1:3382154/1‑69 (MQ=255) gCATCCGGCAGTTGTACTTCATCGAACGGATCACCACCGCGCAAACGGCAATCTTCCATGCGTGCtttt > 1:420987/1‑69 (MQ=255) gCATCCGGCAGTTGTACTTCATCGAACGGATCACCACCGCGCAAACGGCAATCTTCCATGCGTGCtttt > 1:3059343/1‑69 (MQ=255) gCATCCGGCAGTTGTACTTCATCGAACGGATCACCACCGCGCAAACGGCAATCTTCCATGCGTGCtttt > 1:646772/1‑69 (MQ=255) gCATCCGGCAGTTGTACTTCATCGAACGGATCACCACCGCGCAAACGGCAATCTTCCATGCGTGCtttt > 1:2940174/1‑69 (MQ=255) gCATCCGGCAGTTGTACTTCATCGAACGGATCACCACCGCGCAAACGGCAATCTTCCATGCGTGCtttt > 1:765249/1‑69 (MQ=255) gCATCCGGCAGTTGTACTTCATCGAACGGATCACCACCGCGCAAACGGCAATCTTCCATGCGTGCtttt > 1:2619957/1‑69 (MQ=255) gCATCCGGCAGTTGTACTTCATCGAACGGATCACCACCGCGCAAACGGCAATCTTCCATGCGTGCtttt > 1:1372538/1‑69 (MQ=255) gCATCCGGCAGTTGTACTTCATCGAACGGATCACCACCGCGCAAACGGCAATCTTCCATGCGTGCtttt > 1:1385147/1‑69 (MQ=255) gCATCCGGCAGTTGTACTTCATCGAACGGATCACCACCGCGCAAACGGCAATCTTCCATGCGTGCtttt > 1:1766677/1‑69 (MQ=255) tCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAAc < 1:3023428/69‑1 (MQ=255) tGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACaa > 1:297784/1‑46 (MQ=255) tGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTc < 1:3388037/51‑1 (MQ=255) tGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTc < 1:221187/51‑1 (MQ=255) tGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTc < 1:2266175/51‑1 (MQ=255) tGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTc < 1:2777270/51‑1 (MQ=255) tGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg > 1:1580254/1‑69 (MQ=255) tGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg > 1:1848657/1‑69 (MQ=255) tGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg > 1:150283/1‑69 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg < 1:330389/62‑1 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg < 1:2716879/62‑1 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg < 1:2667696/62‑1 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg < 1:1795558/62‑1 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg < 1:1321120/62‑1 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg < 1:1516221/62‑1 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg < 1:2898657/62‑1 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg < 1:2300593/62‑1 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg < 1:426174/62‑1 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg < 1:2938959/62‑1 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg < 1:3148908/62‑1 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg < 1:1289505/62‑1 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGatca > 1:295028/1‑69 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGatca > 1:1840823/1‑69 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGatca > 1:2441812/1‑69 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGatca > 1:429907/1‑69 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGatca > 1:2156353/1‑69 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGatca > 1:2058449/1‑69 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAAAGACAATCTTCCATGCGCGCTTTTAACAGTGGatca > 1:1423318/1‑69 (MQ=255) gCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATgc > 1:1195563/1‑48 (MQ=255) gAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAgg > 1:2986479/1‑68 (MQ=255) gAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAgg > 1:3427274/1‑68 (MQ=255) gAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAgg > 1:1480454/1‑68 (MQ=255) gAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAgg > 1:600611/1‑68 (MQ=255) gAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAgg > 1:663170/1‑68 (MQ=255) gAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAgg > 1:3098724/1‑68 (MQ=255) gAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAgg > 1:1624991/1‑68 (MQ=255) gAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAgg > 1:3017162/1‑68 (MQ=255) gAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAgg > 1:2136300/1‑68 (MQ=255) gAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAgg > 1:241317/1‑68 (MQ=255) gAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAgg > 1:275285/1‑68 (MQ=255) gAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAgg > 1:2844536/1‑68 (MQ=255) gAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAgg > 1:3043167/1‑68 (MQ=255) gAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAgg > 1:1871134/1‑68 (MQ=255) caaCGGGTCGCCACCGCGCAAACGACAATCTTCCa > 1:2363111/2‑35 (MQ=255) caccACCGCGCAAACGGCAATCTTCCATGCGTTCTTTTAATACTGGATCATCCGGCGAGGTAAACGGCa < 1:3216065/67‑1 (MQ=255) caccACCGCGCAAACGGCAATCTTCCATGCGTGCTTTTAATAGTGGATCATCCGGCGAGGTAAACGGCa < 1:2399720/67‑1 (MQ=255) caccACCGCGCAAACGGCAATCTTCCATGCGTGCTTTTAATAGTGGATCATCCGGCGAGGTAAACGGCa < 1:2006091/67‑1 (MQ=255) caccACCGCGCAAACGGCAATCTTCCATGCGTGCTTTTAATAGTGGATCATCCGGCGAGGTAAACGGCa < 1:1421814/67‑1 (MQ=255) caccACCGCGCAAACGGCAATCTTCCATGCGTGCTTTTAATAGTGGATCATCCGGCGAGGTAAACGGCa < 1:2199367/67‑1 (MQ=255) caccACCGCGCAAACGGCAATCTTCCATGCGTGCTTTTAATAGTGGATCATCCGGCGAGGTAAACGGCa < 1:428971/67‑1 (MQ=255) caccACCGCGCAAACGGCAATCTTCCATGCGTGCTTTTAATAGTGGATCATCCGGCGAGGTAAACGGCa < 1:1006284/67‑1 (MQ=255) caccACCGCGCAAACGGCAATCTTCCATGCGTGCTTTTAATAGTGGATCATCCGGCGAGGTAAACGGCa < 1:2535322/67‑1 (MQ=255) caccACCGCGCAAACGGCAATCTTCCATGCGTGCTTTTAATAGTGGATCATCCGGCGAGGTAAACGGCa < 1:1781118/67‑1 (MQ=255) caccACCGCGCAAACGGCAATCTTCCATGCGTGCTTTTAATAGTGGATCATCCGGCGAGGTAAACGGCa < 1:1172921/67‑1 (MQ=255) caccACCGCGCAAACGGCAATCTTCCATGCGTGCTTTTAATAGTGGATCATCCGGCGAGGTAAACGGCa < 1:3163385/67‑1 (MQ=255) caccACCGCGCAAACGGCAATCTTCCATGCGTGCTTTTAATAGTGGATCATCCGGCGAGGTAAACGGCa < 1:2817698/67‑1 (MQ=255) caccACCGCGCAAACGGCAATCTTCCATGCGTGCTTTTAATAGTGGATCATCCGGCGAGGTAAACGGCa < 1:3248214/67‑1 (MQ=255) cGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGc < 1:2195419/68‑1 (MQ=255) gcgcAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTTGTc > 1:1274295/1‑69 (MQ=255) gcgcAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTTGTc > 1:607118/1‑69 (MQ=255) cAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTTGTCGAt < 1:1565001/69‑1 (MQ=255) cAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTTGTCGAt < 1:1751163/69‑1 (MQ=255) cAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTTGTCGAt < 1:3257127/69‑1 (MQ=255) cAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTTGTCGAt < 1:2094635/69‑1 (MQ=255) cAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTAGTCGAt < 1:441649/69‑1 (MQ=255) aaaCGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTTGTCGAt < 1:660227/68‑1 (MQ=255) | TGAGAGTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTTGTCGAT > minE/1195420‑1195543 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |