| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,195,496 | C→T | 28.6% | A552A (GCG→GCA) | yoaA ← | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,195,496 | 0 | C | T | 28.6% | 91.5 / 36.3 | 87 | A552A (GCG→GCA) | yoaA | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| Reads supporting (aligned to +/- strand): ref base C (31/31); new base T (12/13); total (43/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTTGTCGATAATTACCAATGACAATGTA > minE/1195430‑1195562 | gACGGCATCTGGTAGTTGCACTTCATCTAACGGGTCGCCACCGCGCAAACGACAATCTTCCATgcgcg < 1:1240699/68‑1 (MQ=255)gACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATgcgcg < 1:592706/68‑1 (MQ=255) gCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCtttt < 1:2651409/69‑1 (MQ=255) gCATCCGGCAGTTGTACTTCATCGAACGGATCACCACCGCGCAAACGGCAATCTTCCATGCGTGCtttt > 1:2619957/1‑69 (MQ=255) gCATCCGGCAGTTGTACTTCATCGAACGGATCACCACCGCGCAAACGGCAATCTTCCATGCGTGCtttt > 1:765249/1‑69 (MQ=255) gCATCCGGCAGTTGTACTTCATCGAACGGATCACCACCGCGCAAACGGCAATCTTCCATGCGTGCtttt > 1:646772/1‑69 (MQ=255) gCATCCGGCAGTTGTACTTCATCGAACGGATCACCACCGCGCAAACGGCAATCTTCCATGCGTGCtttt > 1:420987/1‑69 (MQ=255) gCATCCGGCAGTTGTACTTCATCGAACGGATCACCACCGCGCAAACGGCAATCTTCCATGCGTGCtttt > 1:371337/1‑69 (MQ=255) gCATCCGGCAGTTGTACTTCATCGAACGGATCACCACCGCGCAAACGGCAATCTTCCATGCGTGCtttt > 1:369199/1‑69 (MQ=255) gCATCCGGCAGTTGTACTTCATCGAACGGATCACCACCGCGCAAACGGCAATCTTCCATGCGTGCtttt > 1:3382154/1‑69 (MQ=255) gCATCCGGCAGTTGTACTTCATCGAACGGATCACCACCGCGCAAACGGCAATCTTCCATGCGTGCtttt > 1:3059343/1‑69 (MQ=255) gCATCCGGCAGTTGTACTTCATCGAACGGATCACCACCGCGCAAACGGCAATCTTCCATGCGTGCtttt > 1:2940174/1‑69 (MQ=255) gCATCCGGCAGTTGTACTTCATCGAACGGATCACCACCGCGCAAACGGCAATCTTCCATGCGTGCtttt > 1:1766677/1‑69 (MQ=255) gCATCCGGCAGTTGTACTTCATCGAACGGATCACCACCGCGCAAACGGCAATCTTCCATGCGTGCtttt > 1:1385147/1‑69 (MQ=255) gCATCCGGCAGTTGTACTTCATCGAACGGATCACCACCGCGCAAACGGCAATCTTCCATGCGTGCtttt > 1:1372538/1‑69 (MQ=255) tCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAAc < 1:3023428/69‑1 (MQ=255) tGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg > 1:150283/1‑69 (MQ=255) tGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg > 1:1580254/1‑69 (MQ=255) tGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg > 1:1848657/1‑69 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg < 1:2716879/62‑1 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg < 1:2667696/62‑1 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg < 1:2898657/62‑1 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg < 1:258581/62‑1 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg < 1:2938959/62‑1 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg < 1:3010453/62‑1 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg < 1:2300593/62‑1 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg < 1:3148908/62‑1 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg < 1:330389/62‑1 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg < 1:2017928/62‑1 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg < 1:1516221/62‑1 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg < 1:1795558/62‑1 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg < 1:426174/62‑1 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg < 1:1289505/62‑1 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg < 1:1321120/62‑1 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGatca > 1:1840823/1‑69 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGatca > 1:429907/1‑69 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGatca > 1:2441812/1‑69 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGatca > 1:295028/1‑69 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGatca > 1:2156353/1‑69 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGatca > 1:2058449/1‑69 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAAAGACAATCTTCCATGCGCGCTTTTAACAGTGGatca > 1:1423318/1‑69 (MQ=255) gAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAgg > 1:3043167/1‑68 (MQ=255) gAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAgg > 1:3017162/1‑68 (MQ=255) gAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAgg > 1:2986479/1‑68 (MQ=255) gAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAgg > 1:3427274/1‑68 (MQ=255) gAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAgg > 1:3098724/1‑68 (MQ=255) gAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAgg > 1:600611/1‑68 (MQ=255) gAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAgg > 1:663170/1‑68 (MQ=255) gAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAgg > 1:1483141/1‑68 (MQ=255) gAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAgg > 1:3408600/1‑68 (MQ=255) gAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAgg > 1:2844536/1‑68 (MQ=255) gAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAgg > 1:1480454/1‑68 (MQ=255) gAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAgg > 1:1871134/1‑68 (MQ=255) gAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAgg > 1:275285/1‑68 (MQ=255) gAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAgg > 1:241317/1‑68 (MQ=255) gAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAgg > 1:1624991/1‑68 (MQ=255) gAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAgg > 1:2136300/1‑68 (MQ=255) caccACCGCGCAAACGGCAATCTTCCATGCGTTCTTTTAATACTGGATCATCCGGCGAGGTAAACGGCa < 1:3216065/67‑1 (MQ=255) caccACCGCGCAAACGGCAATCTTCCATGCGTGCTTTTAATAGTGGATCATCCGGCGAGGTAAACGGCa < 1:2817698/67‑1 (MQ=255) caccACCGCGCAAACGGCAATCTTCCATGCGTGCTTTTAATAGTGGATCATCCGGCGAGGTAAACGGCa < 1:428971/67‑1 (MQ=255) caccACCGCGCAAACGGCAATCTTCCATGCGTGCTTTTAATAGTGGATCATCCGGCGAGGTAAACGGCa < 1:1781118/67‑1 (MQ=255) caccACCGCGCAAACGGCAATCTTCCATGCGTGCTTTTAATAGTGGATCATCCGGCGAGGTAAACGGCa < 1:2006091/67‑1 (MQ=255) caccACCGCGCAAACGGCAATCTTCCATGCGTGCTTTTAATAGTGGATCATCCGGCGAGGTAAACGGCa < 1:3248214/67‑1 (MQ=255) caccACCGCGCAAACGGCAATCTTCCATGCGTGCTTTTAATAGTGGATCATCCGGCGAGGTAAACGGCa < 1:3163385/67‑1 (MQ=255) caccACCGCGCAAACGGCAATCTTCCATGCGTGCTTTTAATAGTGGATCATCCGGCGAGGTAAACGGCa < 1:1172921/67‑1 (MQ=255) caccACCGCGCAAACGGCAATCTTCCATGCGTGCTTTTAATAGTGGATCATCCGGCGAGGTAAACGGCa < 1:2199367/67‑1 (MQ=255) caccACCGCGCAAACGGCAATCTTCCATGCGTGCTTTTAATAGTGGATCATCCGGCGAGGTAAACGGCa < 1:2399720/67‑1 (MQ=255) caccACCGCGCAAACGGCAATCTTCCATGCGTGCTTTTAATAGTGGATCATCCGGCGAGGTAAACGGCa < 1:1421814/67‑1 (MQ=255) caccACCGCGCAAACGGCAATCTTCCATGCGTGCTTTTAATAGTGGATCATCCGGCGAGGTAAACGGCa < 1:2535322/67‑1 (MQ=255) caccACCGCGCAAACGGCAATCTTCCATGCGTGCTTTTAATAGTGGATCATCCGGCGAGGTAAACGGCa < 1:1006284/67‑1 (MQ=255) cGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGc < 1:2195419/68‑1 (MQ=255) gcgcAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTTGTc > 1:607118/1‑69 (MQ=255) gcgcAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTTGTc > 1:1274295/1‑69 (MQ=255) cAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTTGTCGAt < 1:2094635/69‑1 (MQ=255) cAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTTGTCGAt < 1:3257127/69‑1 (MQ=255) cAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTTGTCGAt < 1:1565001/69‑1 (MQ=255) cAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTTGTCGAt < 1:1751163/69‑1 (MQ=255) cAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTAGTCGAt < 1:441649/69‑1 (MQ=255) aaaCGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTTGTCGAt < 1:660227/68‑1 (MQ=255) cTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTTGTCGATAATTACCAAt > 1:2048692/1‑68 (MQ=255) cTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTTGTCGATAATTACCAAt > 1:1733458/1‑68 (MQ=255) cTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTTGTCGATAATTACCAAt > 1:2329717/1‑68 (MQ=255) aTGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTTGTCGATAATTACCAATGAc < 1:40736/66‑1 (MQ=255) aTGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTTGTCGATAATTACCAATGAc < 1:401957/66‑1 (MQ=255) aTGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTTGTCGATAATTACCAATGAc < 1:3249672/66‑1 (MQ=255) aTGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTTGTCGATAATTACCAATGAc < 1:1152070/66‑1 (MQ=255) aTGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTTGTCGATAATTACCAATGAc < 1:2431869/66‑1 (MQ=255) aTGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTTGTCGATAATTACCAATGAc < 1:2655744/66‑1 (MQ=255) tGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTTGTCGATAATTACCAATGAc < 1:2063103/65‑1 (MQ=255) cgcgcTTTTAACATTGGATCATCCGGCGAGGTAAACGGCAATTTGTCGATAATTACCAATGACAATGTa > 1:518347/1‑69 (MQ=255) cgcgcTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTTGTCGATAATTACCAATGACAATGTa > 1:1731542/1‑69 (MQ=255) cgcgcTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTTGTCGATAATTACCAATGACAATGTa > 1:2969172/1‑69 (MQ=255) gcgcTTTTAACAGTGGATCATCCGGCGATGTAAACGGCAATTTGTCGATAATTACCAATGAc < 1:1137056/62‑1 (MQ=255) | GACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTTGTCGATAATTACCAATGACAATGTA > minE/1195430‑1195562 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |