| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,202,586 | G→A | 51.4% | A82T (GCC→ACC) | yoaD → | predicted phosphodiesterase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,202,586 | 0 | G | A | 51.4% | ‑5.1 / 62.0 | 68 | A82T (GCC→ACC) | yoaD | predicted phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base G (8/25); new base A (9/26); total (17/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01 | |||||||||||
ACTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCAAAACTCCAAACTGTGCGATC > minE/1202522‑1202635 | aCTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCa < 1:972433/69‑1 (MQ=255)aCTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCa < 1:2679783/69‑1 (MQ=255)aCTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCa < 1:2679460/69‑1 (MQ=255)aCTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCa < 1:267266/69‑1 (MQ=255)aCTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCa < 1:2595282/69‑1 (MQ=255)aCTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCa < 1:2448008/69‑1 (MQ=255)aCTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCa < 1:2155684/69‑1 (MQ=255)aCTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCTTCCGCTGATTGGGCTGCCCTGCTCTGTCGCCCa < 1:3339850/69‑1 (MQ=255) tCCCCTACAGGCAGTTAGCGAAGTCTTGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGc < 1:312436/68‑1 (MQ=255) tCCCCTACAGGCAGGTAGCGAAGTCTTGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGc < 1:3244347/68‑1 (MQ=255) tCCCCTACAGGCAGGTAGCGAAGTCTTGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGc < 1:2225623/68‑1 (MQ=255) tCCCCTACAGGCAGGTAGCGAAGTCTTGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGc < 1:2007374/68‑1 (MQ=255) ccTACAGGCAGGTAGCGAAGTCTTGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCAt < 1:3309051/68‑1 (MQ=255) ccTACAGGCAGGTAGCGAAGTCTTGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCAt < 1:3210649/68‑1 (MQ=255) ggcaggTAGCGAAGTCTTGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTa < 1:1039395/68‑1 (MQ=255) ggcaggTAGCGAAGTCTTGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTa < 1:1541011/68‑1 (MQ=255) caggTAGCGAAGTCTTGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGc < 1:1454050/57‑1 (MQ=255) aggTAGCGAAGTCTTGCTTCCGCTGATTGGTCTGCCCTTCTCTGTCGCCCATTTGc < 1:1970313/56‑1 (MQ=255) tAGCGAAGTCTTGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAggc > 1:323994/1‑69 (MQ=255) tAGCGAAGTCTTGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAggc > 1:1813993/1‑69 (MQ=255) tAGCGAAGTCTTGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAggc > 1:607559/1‑69 (MQ=255) aGCGAAGTCTTGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAggc > 1:2370722/1‑68 (MQ=255) aGCGAAGTCTTGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAggc > 1:1367640/1‑68 (MQ=255) aGCGAAGTCTTGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAggc > 1:1503345/1‑68 (MQ=255) aGCGAAGTCTTGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAggc > 1:2017409/1‑68 (MQ=255) aaGTCTTGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCaa < 1:621840/69‑1 (MQ=255) aaGTCTTGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCaa < 1:1377379/69‑1 (MQ=255) aaGTCTTGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCaa < 1:2634786/69‑1 (MQ=255) aaGTCTTGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCaa < 1:2697380/69‑1 (MQ=255) cTTGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCAAAAc > 1:2787484/1‑68 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGTCTGCAAAACTc < 1:1901326/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAAccc < 1:1372970/69‑3 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:3097307/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:3227414/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:3158035/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:323362/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:1438002/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:2885389/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:1220755/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:3432097/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:417772/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:645866/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:835322/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:953058/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:980682/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:3026839/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:2960414/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:2958477/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:1691619/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:1596793/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:2258055/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:2356869/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:1501624/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:2652269/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:2589281/69‑1 (MQ=255) tGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:158955/68‑1 (MQ=255) gCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTcc > 1:847771/1‑68 (MQ=255) gCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTcc > 1:2439774/1‑68 (MQ=255) gCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTCCa > 1:181076/1‑69 (MQ=255) gCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTCCa > 1:3233924/1‑69 (MQ=255) gCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTCCa > 1:2778633/1‑69 (MQ=255) gCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTCCa > 1:2848548/1‑69 (MQ=255) gCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTCCa > 1:3151184/1‑69 (MQ=255) gCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTCCa > 1:3008970/1‑69 (MQ=255) gCTCCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTCCa > 1:146271/1‑69 (MQ=255) ttCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCTTTTGc < 1:1032168/39‑1 (MQ=255) ccGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCAATACGTAAACAGGCGGCaa < 1:1661192/58‑1 (MQ=255) ggTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCAAAACTCCAAACTGTGCGATc < 1:271174/68‑1 (MQ=255) | ACTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCAAAACTCCAAACTGTGCGATC > minE/1202522‑1202635 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |