| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,202,612 | G→T | 67.3% | A90A (GCG→GCT) | yoaD → | predicted phosphodiesterase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,202,612 | 0 | G | T | 67.3% | 38.1 / 37.6 | 52 | A90A (GCG→GCT) | yoaD | predicted phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base G (7/10); new base T (9/26); total (16/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.40e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01 | |||||||||||
AAGTCTTGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCAAAACTCCAAACTGTGCGATCCATTGGCCTGGTGCAAGACGGCACACTTTATT > minE/1202548‑1202667 | aaGTCTTGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCaa < 1:2634786/69‑1 (MQ=255)aaGTCTTGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCaa < 1:2697380/69‑1 (MQ=255)aaGTCTTGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCaa < 1:1377379/69‑1 (MQ=255)aaGTCTTGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCaa < 1:621840/69‑1 (MQ=255) cTTGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCAAAAc > 1:2787484/1‑68 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGTCTGCAAAACTc < 1:1901326/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAAccc < 1:1372970/69‑3 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:3227414/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:2652269/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:2885389/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:2958477/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:2960414/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:3026839/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:3097307/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:3158035/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:1220755/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:323362/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:3432097/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:417772/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:645866/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:835322/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:953058/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:980682/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:2589281/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:1438002/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:1501624/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:2356869/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:1596793/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:1691619/69‑1 (MQ=255) ttGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:2258055/69‑1 (MQ=255) tGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTc < 1:158955/68‑1 (MQ=255) gCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTcc > 1:2439774/1‑68 (MQ=255) gCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTcc > 1:847771/1‑68 (MQ=255) gCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTCCa > 1:3008970/1‑69 (MQ=255) gCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTCCa > 1:181076/1‑69 (MQ=255) gCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTCCa > 1:3151184/1‑69 (MQ=255) gCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTCCa > 1:2848548/1‑69 (MQ=255) gCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTCCa > 1:2778633/1‑69 (MQ=255) gCTTCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTCCa > 1:3233924/1‑69 (MQ=255) gCTCCCGCTGATTGGTCTGCCCTGCTCTGTCACCCATTTGCCATTACGTAAACAGGCTGCAAAACTCCa > 1:146271/1‑69 (MQ=255) ccGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCAATACGTAAACAGGCGGCaa < 1:1661192/58‑1 (MQ=255) ggTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCAAAACTCCAAACTGTGCGATc < 1:271174/68‑1 (MQ=255) ccATTTGCCATTACGTAAACAGGCGGCAAAACTCCAAACTGTGCGATCCATTGGCCTGGTGCAAGACgg < 1:2127476/69‑1 (MQ=255) ccATTTGCCATTACGTAAACAGGCGGCAAAACTCCAAACTGTGCGATCCATTGGCCTGGTGCAAGACgg < 1:2282185/69‑1 (MQ=255) ccATTTGCCATTACGTAAACAGGCGGCAAAACTCCAAACTGTGCGATCCATTGGCCTGGTGCAAGACgg < 1:2419795/69‑1 (MQ=255) ccATTTGACATTACGTAAACAGGCGGCAAAACTCCAAACTGTGCGATCCATTGGCCTGGTGCAAGACgg < 1:1096545/69‑1 (MQ=255) tACGTAAACAGGCGGCAAAACTCCAAACTGTGCGATCCATTGGCCTGGTGCAAGACGGCACACTTTAtt > 1:135231/1‑69 (MQ=255) tACGTAAACAGGCGGCAAAACTCCAAACTGTGCGATCCATTGGCCTGGTGCAAGACGGCACACTTTAtt > 1:2768839/1‑69 (MQ=255) tACGTAAACAGGCGGCAAAACTCCAAACTGTGCGATCCATTGGCCTGGTGCAAGACGGCACACTTTAtt > 1:1232555/1‑69 (MQ=255) tACGTAAACAGGCGGCAAAACTCCAAACTGTGCGATCCATTGGCCTGGTGCAAGACGGCACACTTTAtt > 1:2981582/1‑69 (MQ=255) tACGTAAACAGGCGGCAAAACTCCAAACTGTGCGATCCATTGGCCGGGTGCAAGACGGCACACTTTAtt > 1:888002/1‑69 (MQ=255) tACGCAAACAGGCGGCAAAACTCCAAACTGTGCGATCCATTGGCCTGGTGCAAGACGGCACACTTTAtt > 1:123401/1‑69 (MQ=255) | AAGTCTTGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCAAAACTCCAAACTGTGCGATCCATTGGCCTGGTGCAAGACGGCACACTTTATT > minE/1202548‑1202667 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |