| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,204,203 | A→G | 30.7% | T433T (ACT→ACC) | yoaE ← | fused predicted membrane proteins |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,204,203 | 0 | A | G | 30.7% | 31.4 / 15.8 | 39 | T433T (ACT→ACC) | yoaE | fused predicted membrane proteins |
| Reads supporting (aligned to +/- strand): ref base A (9/18); new base G (6/6); total (15/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACA > minE/1204141‑1204263 | tCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATg < 1:3102566/69‑1 (MQ=255)tCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATg < 1:2454376/69‑1 (MQ=255)tCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATg < 1:2778373/69‑1 (MQ=255)tCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATg < 1:2865806/69‑1 (MQ=255)tCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATg < 1:3014538/69‑1 (MQ=255)tCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATg < 1:1908506/69‑1 (MQ=255)tCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATg < 1:1799039/69‑1 (MQ=255)tCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATg < 1:117858/69‑1 (MQ=255) cAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTc > 1:1245710/1‑69 (MQ=255) cAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTc > 1:1645457/1‑69 (MQ=255) cAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTc > 1:3226005/1‑69 (MQ=255) cAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTc > 1:1688342/1‑69 (MQ=255) tCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCggg > 1:1832699/1‑69 (MQ=255) cTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAAt > 1:1237549/1‑40 (MQ=255) cTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGt > 1:1487531/1‑63 (MQ=255) cTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGt > 1:1719805/1‑63 (MQ=255) cTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGt > 1:3170049/1‑63 (MQ=255) gCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCAc < 1:1651824/68‑1 (MQ=255) gCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCAc < 1:1636519/68‑1 (MQ=255) gCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCAc < 1:1979833/68‑1 (MQ=255) gCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCAc < 1:3143396/68‑1 (MQ=255) gCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCAc < 1:101539/68‑1 (MQ=255) gCCTTTTACCAGCAAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCAc < 1:2260514/68‑1 (MQ=255) ttttACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGc < 1:175917/69‑1 (MQ=255) tttACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGc < 1:3229738/68‑1 (MQ=255) aCCAGCCAGCCGTCACCGTCGGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCaa < 1:499194/67‑1 (MQ=255) aCCAGCCAGCCGTCACCGTCGGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCaa < 1:3028778/67‑1 (MQ=255) aCCAGCCAGCCGTCACCGTCGGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCaa < 1:1341004/67‑1 (MQ=255) aCCAGCCAGCCGTCACCGTCGGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCaa < 1:1289739/67‑1 (MQ=255) aCCAGCCAGCCGTCACCGTCGGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCaa < 1:1149597/67‑1 (MQ=255) aCCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGc < 1:16243/65‑1 (MQ=255) ccagccagCCGTCACCGTCGGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCaa < 1:2280017/66‑1 (MQ=255) gccGTCACCATCAGTAATGATTTCCGGCGTTTCGGCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAg < 1:2093909/69‑1 (MQ=255) cACCGTCGGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTc > 1:2781794/1‑61 (MQ=255) cACCGTCGGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCAAGcaca > 1:2991921/1‑68 (MQ=255) cACCGTCGGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCAAGcaca > 1:2092520/1‑68 (MQ=255) cACCGTCGGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCAAGcaca > 1:193428/1‑68 (MQ=255) cACCGTCGGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCAAGcaca > 1:1681331/1‑68 (MQ=255) cACCGTCGGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCAAGcaca > 1:1062105/1‑68 (MQ=255) | TCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACA > minE/1204141‑1204263 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |