| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,204,743 | C→G | 55.2% | L253L (CTG→CTC) | yoaE ← | fused predicted membrane proteins |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,204,743 | 0 | C | G | 55.2% | 12.4 / 62.7 | 58 | L253L (CTG→CTC) | yoaE | fused predicted membrane proteins |
| Reads supporting (aligned to +/- strand): ref base C (18/8); new base G (19/13); total (37/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGCATCGGCATCGGGTTATCGGCATCGTGCTGAACATTGGCCTGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACG > minE/1204677‑1204808 | cggcatcggcatCGGGTTATCGGCATCGTGCTGAACATTGGCCTGACGTTTCCCGCCCATCAAACGCAg < 1:2789713/69‑1 (MQ=255)cggcatcggcatCGGGTTATCGGCATCGTGCTGAACATTGGCCTGACGTTTCCCGCCCATCAAACGCAg < 1:783491/69‑1 (MQ=255)cggcatcggcatCGGGTTATCGGCATCGTGCTGAACATTGGCCTGACGTTTCCCGCCCATCAAACGCAg < 1:3029135/69‑1 (MQ=255) cggcatcggGTTATCGGCATCGTGCTGAACATTGGCCTGACGTTTCCCGCCCATCAAACGCAGGATGGc < 1:1419590/69‑1 (MQ=255) cggcatcggGTTATCGGCATCGTGCTGAACATTGGCCTGACGTTTCCCGCCCATCAAACGCAGGATGGc < 1:1537793/69‑1 (MQ=255) cggGTTATCGGCATCGTGCTGACCATTGGCCTGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGc < 1:1480346/69‑1 (MQ=255) gTTATCGGCATCGTGCTGAACATTGGCCTGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGt > 1:1312214/1‑69 (MQ=255) gTTATCGGCATCGTGCTGAACATTGGCCTGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGt > 1:2903661/1‑69 (MQ=255) gTTATCGGCATCGTGCTGAACATTGGCCTGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGt > 1:332974/1‑69 (MQ=255) gTTATCGGCATCGTGCTGAACATTGGCCTGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGt > 1:3158269/1‑69 (MQ=255) aTCGGCATCGTGCTGAACATTGGCCTGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTAcg > 1:843846/1‑69 (MQ=255) aTCGGCATCGTGCTGAACATTGGCCTGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTAcg > 1:1191345/1‑69 (MQ=255) aTCGGCATCGTGCTGAACATTGGCCTGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTAcg > 1:1664207/1‑69 (MQ=255) aTCGGCATCGTGCTGAACATTGGCCTGACGTTTCCCGCCCATCAAACGCAGGATGGCATAGGCAGTAcg > 1:2278632/1‑69 (MQ=255) ggCATCGTGCTGAACATTGGCCTGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGc < 1:1657365/60‑1 (MQ=255) gTGCTGCACATTGGCCTGACGTTTCCCGCCCATCAAACGCAGGATGGCATc > 1:1025726/1‑51 (MQ=255) gTGCTGAACATTGGCCTGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAg > 1:3212406/1‑69 (MQ=255) gTGCTGAACATTGGCCTGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAg > 1:1709338/1‑69 (MQ=255) gTGCTGAACATTGGCCTGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAg > 1:2653502/1‑69 (MQ=255) cTGACGTTTCCCGCCCATCAAGCGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACtg > 1:1893710/1‑69 (MQ=255) cTGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACtg > 1:1633639/1‑69 (MQ=255) cTGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACtg > 1:469994/1‑69 (MQ=255) cTGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACtg > 1:2201919/1‑69 (MQ=255) cTGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACtg > 1:1130031/1‑69 (MQ=255) cTGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACt > 1:2769576/1‑68 (MQ=255) tGACGTTTCCCGCCCTTCAAACGCAGGATGGCATCGGc < 1:2740838/38‑1 (MQ=255) gACGTTTCCCGCCCATCAAACGGAGAATCGCATCGGCAGTACGCGCTCGCAGCGGTAAAGTCGACtggt < 1:2859854/69‑1 (MQ=255) gACGTTTCCCGCCCATCAAACGGAGAATCGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACtggt < 1:3243016/69‑1 (MQ=255) gACGTTTCCCGCCCATCAAACGGAGAATCGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACtggt < 1:510123/69‑1 (MQ=255) gACGTTTCCCGCCCATCAAACGGAGAATCGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACtggt < 1:2841595/69‑1 (MQ=255) gACGTTTCCCGCCCATCAAACGGAGAATCGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACtggt < 1:2831930/69‑1 (MQ=255) gACGTTTCCCGCCCATCAAACGGAGAATCGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACtggt < 1:1042242/69‑1 (MQ=255) gACGTTTCCCGCCCATCAAACGGAGAATCGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACtggt < 1:1394326/69‑1 (MQ=255) gACGTTTCCCGCCCATCAAACGGAGAATCGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACtggt < 1:2377322/69‑1 (MQ=255) gACGTTTCCCGCCCATCAAACGGAGAATCGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACtggt < 1:2154738/69‑1 (MQ=255) gACGTTTCCCGCCCATCAAACGGAGAATCGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACtggt < 1:20029/69‑1 (MQ=255) gACGTTTCCCGCCCATCAAACGGAGAATCGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACtggt < 1:1861464/69‑1 (MQ=255) gACGTTTCCCGCCCATCAAACGGAGAATCGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACtggt < 1:1400201/69‑1 (MQ=255) tCCCGCCCATCAAACGGAGAATCGCATCGGCAGTACGCGCTCGCAGCGGAAAAGTCGACtggt < 1:594/63‑1 (MQ=255) aCGGAGAATCGCATCGGCAGTACTCGCTCGCAGCGGCAAAGTCGACTGGTGACGAATAAAGTTGCGAcg > 1:2241782/1‑69 (MQ=255) aCGGAGAATCGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGACGAATAAAGTTGCGAcg > 1:1609116/1‑69 (MQ=255) aCGGAGAATCGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGACGAATAAAGTTGCGAcg > 1:1072883/1‑69 (MQ=255) aCGGAGAATCGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGACGAATAAAGTTGCGAcg > 1:1284114/1‑69 (MQ=255) aCGGAGAATCGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGACGAATAAAGTTGCGAcg > 1:544389/1‑69 (MQ=255) aCGGAGAATCGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGACGAATAAAGTTGCGAcg > 1:1364065/1‑69 (MQ=255) aCGGAGAATCGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGACGAATAAAGTTGCGAcg > 1:402850/1‑69 (MQ=255) aCGGAGAATCGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGACGAATAAAGTTGCGAcg > 1:1406303/1‑69 (MQ=255) aCGGAGAATCGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGACGAATAAAGTTGCGAcg > 1:1439178/1‑69 (MQ=255) aCGGAGAATCGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGACGAATAAAGTTGCGAcg > 1:1483924/1‑69 (MQ=255) aCGGAGAATCGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGACGAATAAAGTTGCGAcg > 1:3032223/1‑69 (MQ=255) aCGGAGAATCGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGACGAATAAAGTTGCGAcg > 1:262331/1‑69 (MQ=255) aCGGAGAATCGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGACGAATAAAGTTGCGAcg > 1:2797039/1‑69 (MQ=255) aCGGAGAATCGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGACGAATAAAGTTGCGAcg > 1:277848/1‑69 (MQ=255) aCGGAGAATCGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGACGAATAAAGTTGCGAcg > 1:2497040/1‑69 (MQ=255) aCGGAGAATCGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGACGAATAAAGTTGCGAcg > 1:2757838/1‑69 (MQ=255) aCGGAGAATCGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGACGAATAAAGTTGCGAcg > 1:2751009/1‑69 (MQ=255) aCGGAGAATCGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGACGAATAAAGTTGCGAcg > 1:2749327/1‑69 (MQ=255) aCGGAGAATCGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGACGAATAAAGTTGCGAcg > 1:2502853/1‑69 (MQ=255) | CGGCATCGGCATCGGGTTATCGGCATCGTGCTGAACATTGGCCTGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACG > minE/1204677‑1204808 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |