| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 99,507 | T→C | 39.2% | T320A (ACC→GCC) | hofC ← | assembly protein in type IV pilin biogenesis, transmembrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 99,507 | 0 | T | C | 39.2% | 38.1 / 57.1 | 84 | T320A (ACC→GCC) | hofC | assembly protein in type IV pilin biogenesis, transmembrane protein |
| Reads supporting (aligned to +/- strand): ref base T (34/17); new base C (19/14); total (53/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01 | |||||||||||
CCAGAGAGCCGGATGCCTCTCCTGTTCTCACTAATTGCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGAA > minE/99444‑99569 | ccAGAGAGCCGGATGCCTCTCCTGTTCTCACTAATTGCAAACAGAGCGGGCTAAACTCACCGGTAttt > 1:850760/1‑68 (MQ=255) tGCCTCTCCTGTTCTCACTAATTGCAAGCAGAGCGGGCTAAACTCACCGGTATTTTTTAGCgccagcca < 1:2801608/69‑1 (MQ=255) tGCCTCTCCTGTTCTCACTAATTGCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCgccagcca < 1:2147993/69‑1 (MQ=255) tGCCTCTCCTGTTCTCACTAATTGCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCgccagcca < 1:1851431/69‑1 (MQ=255) tGCCTCTCCTGTTCTCACTAATTGCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCgccagcca < 1:2144100/69‑1 (MQ=255) tGCCTCTCCTGTTCTCACTAATTGCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCgccagcca < 1:1368005/69‑1 (MQ=255) tGCCTCTCCTGTGCTCACTAATTGCAAACAGAGCTGGCTAAACTCACCGGTATTTTTTAGCgccagcca < 1:1634739/69‑1 (MQ=255) gCCTCTCCTGTTCTCACTAATTGCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCGccagccag > 1:226513/1‑69 (MQ=255) gCCTCTCCTGTTCTCACTAATTGCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCGccagccag > 1:2778613/1‑69 (MQ=255) gCCTCTCCTGTTCTCACTAATTGCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCGccagccag > 1:174849/1‑69 (MQ=255) gCCTCTCCTGTTCTCACTAATTGCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCGccagccag > 1:929528/1‑69 (MQ=255) ctcCTGTTCTCACTAATTGCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCgccagcca > 1:467390/1‑64 (MQ=255) ctcCTGTTCTCACTAATTGCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCgccagcca > 1:2564701/1‑64 (MQ=255) ctcCTGTTCTCACTAATTGCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCgccagcca > 1:574756/1‑64 (MQ=255) ctcCTGTTCTCACTAATTGCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCgccagcca > 1:2045418/1‑64 (MQ=255) ctcCTGTTCTCACTAATTGCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCgccagcca > 1:1932058/1‑64 (MQ=255) ctcCTGTTCTCACTAATTGCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCgccagcca > 1:1152115/1‑64 (MQ=255) ctcCTGTTCTCACTAATTGCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCgccagcca > 1:3412363/1‑64 (MQ=255) ctcCTGTTCTCACTAATTGCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCgccagcca > 1:2984836/1‑64 (MQ=255) ctcCTGTTCTCACTAATTGCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATgg < 1:2381645/69‑1 (MQ=255) ctcCTGTTCTCACTAATCGCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCgccagcca > 1:2303308/1‑64 (MQ=255) ctcATGTTCTCACTAATTGCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATgg < 1:2960310/69‑1 (MQ=255) cTGTTCTCACTAATTGCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATGGGtt > 1:1998230/1‑69 (MQ=255) tGTTCTCACTAATTGCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCgccagcca < 1:1334743/60‑1 (MQ=255) ctcACTAATTGCAAACAGAGCGGGCTAAACTCACCGGTAt > 1:3300513/1‑40 (MQ=255) ctcACTAATTGCAAACAGAGCGGGCTAAACTCACCGGTAt > 1:1328664/1‑40 (MQ=255) ctcACTAATTGCAAACAGAGCGGGCTAAACTCACCGGTAt > 1:1534384/1‑40 (MQ=255) ctcACTAATTGCAAACAGAGCGGGCTAAACTCACCGGTAt > 1:1515827/1‑40 (MQ=255) ctcACTAATTGCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGAcc > 1:1864966/1‑68 (MQ=255) cACTAATTTCAAACAGAGCGGGCTAAACTCACCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGt < 1:373994/68‑1 (MQ=255) cACTAATTGCAAACAGAGCGGGCTAAACTCACCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGt < 1:1959136/68‑1 (MQ=255) cACTAATTGCAAACAGAGCGGGCTAAACTCACCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGt < 1:873958/68‑1 (MQ=255) cACTAATTGCAAACAGAGCGGGCTAAACTCACCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGt < 1:2246421/68‑1 (MQ=255) cACTAATTGCAAACAGAGCGGGCTAAACTCACCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGt < 1:1317550/68‑1 (MQ=255) cACTAATTGCAAACAGAGCGGGCTAAACTCACCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGt < 1:3223972/68‑1 (MQ=255) cACTAATTGCAAACAGAGCGGGCTAAACTCACCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGt < 1:2221395/68‑1 (MQ=255) cACTAATTGCAAACAGAGCGGGCTAAACTCACCGGCATTTTTTAGCGCCAGCCAGATGGGGCGACCGt < 1:4119/68‑1 (MQ=255) aCTAATTGCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTa > 1:3176997/1‑69 (MQ=255) tGCAAACAGAGCGGGCTAAACTCACCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGata < 1:377610/69‑1 (MQ=255) tGCAAACAGAGCGGGCTAAACTCACCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGata < 1:1635903/69‑1 (MQ=255) tGCAAACAGAGCGGGCTAAACTCACCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGata < 1:454023/69‑1 (MQ=255) tGCAAACAGAGCGGGCTAAACTCACCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGata < 1:1916012/69‑1 (MQ=255) tGCAAACAGAGCGGGCTAAACTCACCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGata < 1:1201037/69‑1 (MQ=255) gCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTa > 1:2087706/1‑36 (MQ=255) gCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGatat > 1:806236/1‑69 (MQ=255) gCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGatat > 1:2351385/1‑69 (MQ=255) gCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGatat > 1:3322153/1‑69 (MQ=255) gCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGatat > 1:2418175/1‑69 (MQ=255) gCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGatat > 1:895724/1‑69 (MQ=255) gCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGatat > 1:1959870/1‑69 (MQ=255) gCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGatat > 1:876783/1‑69 (MQ=255) gCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGatat > 1:1183991/1‑69 (MQ=255) gCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGatat > 1:188407/1‑69 (MQ=255) gCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGatat > 1:2981305/1‑69 (MQ=255) gCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGata > 1:3321966/1‑68 (MQ=255) gCAAACAGAGCGGGCTAAACTCACCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGata < 1:1706735/68‑1 (MQ=255) aaaCAGAGCGGGCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATgg < 1:804753/49‑1 (MQ=255) ggCTAAACTCACC‑GTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGATATCGTGCTGGATTtg < 1:2791519/69‑1 (MQ=255) gCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGATATCGTGCTGGATTtg < 1:2244418/69‑1 (MQ=255) gCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGATATCGTGCTGGATTtg < 1:1486001/69‑1 (MQ=255) gCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGATATCGTGCTGGATTtg < 1:767939/69‑1 (MQ=255) gCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGATATCGTGCTGGATTtg < 1:196537/69‑1 (MQ=255) gCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGATATCGTGCTGGATTtg < 1:1982867/69‑1 (MQ=255) aaaCTCACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGATATCGTGCTGGATTTgtgt < 1:2262858/69‑1 (MQ=255) cACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGaa > 1:1265049/1‑69 (MQ=255) aCCGGCATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGaa > 1:2149089/1‑68 (MQ=255) aCCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGaa > 1:497461/1‑68 (MQ=255) aCCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGaa > 1:1094611/1‑68 (MQ=255) aCCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGaa > 1:970722/1‑68 (MQ=255) aCCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGaa > 1:3075169/1‑68 (MQ=255) aCCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGaa > 1:2790698/1‑68 (MQ=255) aCCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGaa > 1:2631769/1‑68 (MQ=255) aCCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGaa > 1:2607989/1‑68 (MQ=255) aCCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGaa > 1:2464275/1‑68 (MQ=255) aCCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGaa > 1:2348154/1‑68 (MQ=255) aCCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGaa > 1:211963/1‑68 (MQ=255) aCCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGaa > 1:2057736/1‑68 (MQ=255) aCCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGaa > 1:200294/1‑68 (MQ=255) aCCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGaa > 1:1988884/1‑68 (MQ=255) aCCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGaa > 1:1153097/1‑68 (MQ=255) aCCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGaa > 1:1104205/1‑68 (MQ=255) aCCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGa > 1:3187159/1‑67 (MQ=255) aCCGGCATTTTTTAGCCCCAGCCAGATGGGGTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGaa > 1:930179/1‑68 (MQ=255) aCCGGCATTTTTTAGCCCCAGCCAGATGGGGTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGaa > 1:934087/1‑68 (MQ=255) | CCAGAGAGCCGGATGCCTCTCCTGTTCTCACTAATTGCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGAA > minE/99444‑99569 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |