| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,211,750 | A→T | 41.5% | intergenic (‑243/+427) | yobF ← / ← yebO | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,211,750 | 0 | A | T | 41.5% | 37.4 / 80.7 | 82 | intergenic (‑243/+427) | yobF/yebO | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (31/17); new base T (12/22); total (43/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.33e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 | |||||||||||
AGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACA‑TTTTTTGCTATGCAATGAATAAAAAGT > minE/1211683‑1211814 | aGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAg < 1:789371/69‑1 (MQ=255) tACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTT‑CAGGCCAGTGGGAGAAGttat < 1:1100563/69‑1 (MQ=255) aCTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGttat < 1:3179985/69‑1 (MQ=255) aCTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGttat < 1:723131/69‑1 (MQ=255) cTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAAAAGttat > 1:2321156/1‑68 (MQ=255) cTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAAAAGttat > 1:303124/1‑68 (MQ=255) cTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAAAAGttat > 1:2714167/1‑68 (MQ=255) cTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAAAAGttat > 1:1226399/1‑68 (MQ=255) cTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAAAAGttat > 1:484342/1‑68 (MQ=255) cTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAAAAGttat > 1:1149396/1‑68 (MQ=255) cTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAAAAGttat > 1:739421/1‑68 (MQ=255) tAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGttatta > 1:129461/1‑68 (MQ=255) tAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGttatta > 1:2675031/1‑68 (MQ=255) tAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGttatta > 1:1069975/1‑68 (MQ=255) tAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGttatta > 1:858711/1‑68 (MQ=255) aCTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACa < 1:2184990/67‑1 (MQ=255) aCTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACa < 1:2686456/67‑1 (MQ=255) aCTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACa < 1:3401592/67‑1 (MQ=255) aCTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACa < 1:138169/67‑1 (MQ=255) aCTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACa < 1:578794/67‑1 (MQ=255) aCTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATa > 1:3089134/1‑69 (MQ=255) aCTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATa > 1:420432/1‑69 (MQ=255) aCTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATa > 1:2344348/1‑69 (MQ=255) aCTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATa > 1:1050952/1‑69 (MQ=255) cTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACa < 1:634800/66‑1 (MQ=255) ttttCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGttatta > 1:3187705/1‑56 (MQ=255) ttttCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGttatta > 1:3183499/1‑56 (MQ=255) ttttCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGttatta > 1:66663/1‑56 (MQ=255) ttCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCaa > 1:3140789/1‑68 (MQ=255) ttCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCaa > 1:1991689/1‑68 (MQ=255) ttCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAat > 1:621614/1‑69 (MQ=255) ttCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAat > 1:3229862/1‑69 (MQ=255) ttCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAat > 1:345977/1‑69 (MQ=255) ttCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAat > 1:1097373/1‑69 (MQ=255) gTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACA‑tttt > 1:1504185/1‑69 (MQ=255) gTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACA‑tttt > 1:697338/1‑69 (MQ=255) gTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACA‑tttt > 1:2469407/1‑69 (MQ=255) gTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACA‑tttt > 1:1079616/1‑69 (MQ=255) cAATAAGTTCAGTTGTTCCAGGCCTGTGGGAGAAGTTATTACATatt > 1:3267992/1‑45 (MQ=255) cAATAAGTTCAGTTGTTCCAGGCCTGTGGGAGAAGTTATTACATAgtgcgtgc > 1:2704187/1‑53 (MQ=255) cAATAAGTTCAGTTGTTCCAGGCCTGTGGGAGAAGTTATTACATAgtgcgtgc > 1:2218451/1‑53 (MQ=255) cAATAAGTTCAGTTGTTCCAGGCCTGTGGGAGAAGTTATTACATAgtgcgtgc > 1:329168/1‑53 (MQ=255) cAATAAGTTCAGTTGTTCCAGGCCTGTGGGAGAAGTTATTACATAgtgcgtgc > 1:3410275/1‑53 (MQ=255) cAATAAGTTCAGTTGTTCCAGGCCTGTGGGAGAAGTTATTACATAgtgcgtgc > 1:981109/1‑53 (MQ=255) cAATAAGTTCAGTTGTTCCAGGCCTGTGGGAGAAGTTATTACATAgtgcgtgc > 1:1893826/1‑53 (MQ=255) cAATAAGTTCAGTTGTTCCAGGCCTGTGGGAGAAGTTATTACATAgtgcgtgc > 1:2046657/1‑53 (MQ=255) cAATAAGTTCAGTTGTTCCAGGCCTGTGGGAGAAGTTATTACATAgtgcgtgc > 1:2223069/1‑53 (MQ=255) cAATAAGTTCAGTTGTTCCAGGCCTGTGGGAGAAGTTATTACATAgtgcgtgc > 1:2362422/1‑53 (MQ=255) cAATAAGTTCAGTTGTTCCAGGCCTGTGGGAGAAGTTATTACATAgtgcgtgc > 1:2375024/1‑53 (MQ=255) cAATAAGTTCAGTTGTTCCAGGCCTGTGGGAGAAGTTATTACATAgtgcgtgc > 1:2465531/1‑53 (MQ=255) tAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACA‑TTTTTTGCt < 1:1192016/68‑1 (MQ=255) aaGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACA‑TTTTTTGCt < 1:2040984/67‑1 (MQ=255) gttgttCCAGGCCTGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACA‑TTTTCTACTATGCAATg < 1:2353245/68‑1 (MQ=255) gttgttCCAGGCCTGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACA‑TTTTCTACTATGCAATg < 1:2695043/68‑1 (MQ=255) gttgttCCAGGCCTGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACA‑TTTTCTACTATGCAATg < 1:919057/68‑1 (MQ=255) gttgttCCAGGCCTGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACA‑TTTTCTACTATGCAATg < 1:2707008/68‑1 (MQ=255) gttgttCCAGGCCTGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACA‑TTTTCTACTATGCAATg < 1:2327263/68‑1 (MQ=255) gttgttCCAGGCCTGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACA‑TTTTCTACTATGCAATg < 1:629234/68‑1 (MQ=255) gttgttCCAGGCCTGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACA‑TTTTCTACTATGCAATg < 1:2350875/68‑1 (MQ=255) gttgttCCAGGCCTGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACA‑TTTTCTACTATGCAATg < 1:1159031/68‑1 (MQ=255) gttgttCCAGGCCTGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACA‑TTTTCTACTATGCAATg < 1:395411/68‑1 (MQ=255) gttgttCCAGGCCTGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACA‑TTTTCTACTATGCAATg < 1:387035/68‑1 (MQ=255) gttgttCCAGGCCTGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACA‑TTTTCTACTATGCAATg < 1:1972384/68‑1 (MQ=255) gttgttCCAGGCCTGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACA‑TTTTCTACTATGCAATg < 1:180345/68‑1 (MQ=255) gttgttCCAGGCCTGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACA‑TTTTCTACTATGCAATg < 1:2676686/68‑1 (MQ=255) gttgttCCAGGCCTGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACA‑TTTTCTACTATGCAATg < 1:1776012/68‑1 (MQ=255) gttgttCCAGGCCTGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACA‑TTTTCTACTATGCAATg < 1:1873650/68‑1 (MQ=255) gttgttCCAGGCCTGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACA‑TTTTCTACTATGCAATg < 1:2565252/68‑1 (MQ=255) gttgttCCAGGCCTGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACA‑TTTTCTACTATGCAATg < 1:2515493/68‑1 (MQ=255) gttgttCCAGGCCTGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACA‑TTTTCTACTATGCAATg < 1:3022711/68‑1 (MQ=255) gttgttCCAGGCCTGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACA‑TTTTCTACTATGCAATg < 1:3394012/68‑1 (MQ=255) gttgttCCAGGCCTGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACA‑TTTTCTACTATGCAATg < 1:2802547/68‑1 (MQ=255) gttgttCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACA‑TTTTTTGCTATGCAATg < 1:1614977/68‑1 (MQ=255) ttgttCCAGGCCTGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACA‑TTTTCTACTATGCAATg < 1:1864936/67‑1 (MQ=255) ttgttCCAGGCCTCTGGGAGAAGTTATTACATAGTGCGTGCAATATCACA‑TTTTCTACTATGCAATg < 1:940108/67‑1 (MQ=255) ttgttCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTTGCTATGCAATg < 1:2303536/68‑1 (MQ=255) ccAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACA‑TTTTTTGCTATGCAATg > 1:372052/1‑62 (MQ=255) ccAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACA‑TTTTTTGCTATGCAATGaa > 1:2240240/1‑64 (MQ=255) ccAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACA‑TTTTTTGCTATGCAATGAATaaaa > 1:734747/1‑69 (MQ=255) ggCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACA‑TTTTTTGCTATGCAATGAATAAAAAGt < 1:247401/69‑1 (MQ=255) ggCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACA‑TTTTTTGCTATGCAATGAATAAAAAGt < 1:1070278/69‑1 (MQ=255) ggCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACA‑TTTTTTGCTATGCAATGAATAAAAAGt < 1:100767/69‑1 (MQ=255) | AGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACA‑TTTTTTGCTATGCAATGAATAAAAAGT > minE/1211683‑1211814 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |