| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,217,488 | G→C | 45.4% | P415P (CCC→CCG) | prc ← | carboxy‑terminal protease for penicillin‑binding protein 3 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,217,488 | 0 | G | C | 45.4% | 21.9 / 111.6 | 97 | P415P (CCC→CCG) | prc | carboxy‑terminal protease for penicillin‑binding protein 3 |
| Reads supporting (aligned to +/- strand): ref base G (25/28); new base C (33/11); total (58/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.92e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.07e-02 | |||||||||||
TCCGTCGGTATCGCTATCTTCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATAAACAGACCGGAGAGCGATACGGCTTCAGTTAACGCCCCACCGCC > minE/1217431‑1217547 | tCCGTCGGTATCGCTATCTTCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAgg > 1:2368468/1‑69 (MQ=255)tCCGTCGGTATCGCTATCTTCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAgg > 1:864169/1‑69 (MQ=255) tCGGTATCGCTATCTTCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGaaaa < 1:2625005/69‑3 (MQ=255) tCGGTATCGCTATCTTCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATa < 1:722899/69‑1 (MQ=255) tCGGTATCGCTATCTTCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATa < 1:1696575/69‑1 (MQ=255) tCGGTATCGCTATCTTCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATa < 1:1425621/69‑1 (MQ=255) tCGGTATCGCTATCTTCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATa < 1:1105453/69‑1 (MQ=255) tCTTCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATCGGGCCGGATGGAATGAACAGACCGgag > 1:1210925/1‑69 (MQ=255) tCTTCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATCGGGCCGGATGGAATGAACAGACCGgag > 1:3337067/1‑69 (MQ=255) tCTTCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATCGGGCCGGATGGAATGAACAGACCGgag > 1:1621263/1‑69 (MQ=255) tCTTCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATCGGGCCGGATGGAATGAACAGACCGgag > 1:1932229/1‑69 (MQ=255) tCTTCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATCGGGCCGGATGGAATGAACAGACCGgag > 1:1602530/1‑69 (MQ=255) tCTTCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATCGGGCCGGATGGAATGAACAGACCGgag > 1:2535444/1‑69 (MQ=255) tCTTCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATCGGGCCGGATGGAATGAACAGACCGgag > 1:2555550/1‑69 (MQ=255) tCTTCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATCGGGCCGGATGGAATGAACAGACCGgag > 1:1528188/1‑69 (MQ=255) tCTTCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATCGGGCCGGATGGAATGAACAGACCGgag > 1:3341397/1‑69 (MQ=255) tCTTCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATCGGGCCGGATGGAATGAACAGACCGgag > 1:2822046/1‑69 (MQ=255) tCTTCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATCGGGCCGGATGGAATGAACAGACCGgag > 1:1440988/1‑69 (MQ=255) tCTTCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATCGGGCCGGATGGAATGAACAGACCGgag > 1:883503/1‑69 (MQ=255) tCTTCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATCGGGCCGGATGGAATGAACAGACCGgag > 1:2190429/1‑69 (MQ=255) tCTTCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATCGGGCCGGATGGAATGAACAGACCGgag > 1:2661281/1‑69 (MQ=255) tCTTCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATCGGGCCGGATGGAATGAACAGACCGgag > 1:1788703/1‑69 (MQ=255) tCTTCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATCGGGCCGGATGGAATGAACAGACCGgag > 1:772065/1‑69 (MQ=255) tCTTCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATCGGGCCGGATGGAATGAACAGACCGgag > 1:3381299/1‑69 (MQ=255) ttCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATa > 1:3393470/1‑55 (MQ=255) ttCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATa > 1:3003160/1‑55 (MQ=255) ttCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATa < 1:2362679/55‑1 (MQ=255) ttCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATa < 1:3260708/55‑1 (MQ=255) ttCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATa < 1:3116489/55‑1 (MQ=255) ttCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATa < 1:3090769/55‑1 (MQ=255) ttCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATa < 1:2813141/55‑1 (MQ=255) ttCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATa > 1:3081916/1‑55 (MQ=255) ttCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATa > 1:3073639/1‑55 (MQ=255) ttCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATa > 1:1390752/1‑55 (MQ=255) ttCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATa < 1:1945540/55‑1 (MQ=255) ttCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATa > 1:1051372/1‑55 (MQ=255) ttCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATa > 1:365180/1‑55 (MQ=255) ttCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATa > 1:1358199/1‑55 (MQ=255) ttCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATa < 1:3411079/55‑1 (MQ=255) ttCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATa < 1:410175/55‑1 (MQ=255) ttCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATa > 1:900085/1‑55 (MQ=255) ttCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATa > 1:1642763/1‑55 (MQ=255) ttCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATAAACAGACCGgaga > 1:735155/1‑68 (MQ=255) ttCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATAAACAGACCGgaga > 1:3198642/1‑68 (MQ=255) ttCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATAAACAGACCGgaga > 1:10806/1‑68 (MQ=255) ttCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATAAACAGACCGgaga > 1:177582/1‑68 (MQ=255) ttCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATAAACAGACCGgaga > 1:2008149/1‑68 (MQ=255) ttCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATAAACAGACCGgag > 1:1616343/1‑67 (MQ=255) ttCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATAAACAGACCGgag < 1:2220293/67‑1 (MQ=255) ttCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATAAACAGACCGgag < 1:2008486/67‑1 (MQ=255) cGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATAAACAGACCGGAGAGCGa < 1:1105411/68‑1 (MQ=255) cGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATAAACAGACCGGAGAGCGa < 1:3280361/68‑1 (MQ=255) cGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATAAACAGACCGGAGAGCGa < 1:651067/68‑1 (MQ=255) cGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATAAACAGACCGGAGAGCGa < 1:146929/68‑1 (MQ=255) cGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATAAACAGACCGGAGAGCGa < 1:36295/68‑1 (MQ=255) ttGCCGTTGTTATCGCGGACCTGAACAATCGGGccg > 1:1529623/1‑35 (MQ=37) ttGCCGTTGTTATCGCGGACCTGAACAATCGGGCCGGATGGAATGACCAGACCGGAGAGCGa > 1:467881/1‑62 (MQ=255) ttGCCGTTGTTATCGCGGACCTGAACAATCGGGCCGGATGGAATGAACa > 1:2335612/1‑49 (MQ=255) ttGCCGTTGTTATCGCGGACCTGAACAATCGGGCCGGATGGAATGAACAg > 1:2570805/1‑50 (MQ=255) ttGCCGTTGTTATCGCGGACCTGAACAATCGGGCCGGATGGAATGAACAGACCGGAGAGCGa > 1:1027734/1‑62 (MQ=255) ttGCCGTTGTTATCGCGGACCTGAACAATCGGGCCGGATGGAATGAACAGACCGGAGAGCGa > 1:447620/1‑62 (MQ=255) ttGCCGTTGTTATCGCGGACCTGAACAATCGGGCCGGATGGAATGAACAGACCGGAGAGCGa > 1:196345/1‑62 (MQ=255) ttGCCGTTGTTATCGCGGACCTGAACAATCGGGCCGGATGGAATGAACAGACCGGAGAGCGa > 1:2955909/1‑62 (MQ=255) ttGCCGTTGTTATCGCGGACCTGAACAATCGGGCCGGATGGAATGAACAGACCGGAGAGCGa > 1:2090072/1‑62 (MQ=255) ttGCCGTTGTTATCGCGGACCTGAACAATCGGGCCGGATGGAATGAACAGACCGGAGAGCGa > 1:557397/1‑62 (MQ=255) ttGCCGTTGTTATCGCGGACCTGAACAATCGGGCCGGATGGAATGAACAGACCGGAGAGCGa > 1:3298161/1‑62 (MQ=255) ttGCCGTTGTTATCGCGGACCTGAACAATCGGGCCGGATGGAATGAACAGACCGGAGAGCGa > 1:634805/1‑62 (MQ=255) ttGCCGTTGTTATCGCGGACCTGAACAATCGGGCCGGATGGAATGAACAGACCGGAGAGCGa > 1:1459989/1‑62 (MQ=255) ttGCCGTTGTTATCGCGGACCTGAACAATCGGGCCGGATGGAATGAACAGACCGGAGAGCGa > 1:1084814/1‑62 (MQ=255) ttGCCGTTGTTATCGCGGACCTGAACAATCGGGCCGGATGGAATGAACAGACCGGAGAGCGa > 1:1062778/1‑62 (MQ=255) ttGCCGTTGTTATCGCGGACCAGAACAATCGGGCCGGATGGAATGAACAGACCGGAGAGCGa > 1:1570744/1‑62 (MQ=255) ccGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATa < 1:1807803/42‑1 (MQ=255) cGTTGTTATCGCTGACCTGAACAATGGGACCCGCAGGAATAAACAGACCGGAGAGCGATACGGCTTc < 1:2233749/67‑1 (MQ=255) cGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATAAACAGACCGGAGAGCGATACGGCTTc < 1:2541120/67‑1 (MQ=255) cGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATAAACAGACCGGAGAGCGATACGGCTTc < 1:2643548/67‑1 (MQ=255) cGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATAAACAGACCGGAGAGCGATACGGCTTc < 1:1390802/67‑1 (MQ=255) cGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATAAACAGACCGGAGAGCGATACGGCTTc < 1:2921053/67‑1 (MQ=255) ttgttATCGCGGACCTGAACAATGGGACCCGCAGGAATAAACAGACCGGAGAGCGATACGGCTTCAGtt < 1:2581977/69‑1 (MQ=255) aTCGCGGACCTGAACAATCGGGCCGGATGGAATGAACAGACCGGAGAGCGATACGGCTTCAGTCAACGc < 1:722528/69‑1 (MQ=255) aTCGCGGACCTGAACAATCGGGCCGGATGGAATGAACAGACCGGAGAGCGATACGGCTTCAGTCAACGc < 1:2008691/69‑1 (MQ=255) aTCGCGGACCTGAACAATCGGGCCGGATGGAATGAACAGACCGGAGAGCGATACGGCTTCAGTCAACGc < 1:1395845/69‑1 (MQ=255) aTCGCGGACCTGAACAATCGGGCCGGATGGAATGAACAGACCGGAGAGCGATACGGCTTCAGTCAACGc < 1:1090129/69‑1 (MQ=255) aTCGCGGACCTGAACAATCGGGCCGGATGGAATGAACAGACCGGAGAGCGATACGGCTTCAGTCAACGc < 1:1718920/69‑1 (MQ=255) aTCGCGGACCTGAACAATCGGGCCGGATGGAATGAACAGACCGGAGAGCGATACGGCTTCAGTCAACGc < 1:1784674/69‑1 (MQ=255) aTCGCGGACCTGAACAATCGGGCCGGATGGAATGAACAGACCGGAGAGCGATACGGCTTCAGTCAACGc < 1:2712975/69‑1 (MQ=255) aTCGCGGACCTGAACAATCGGGCCGGATGGAATGAACAGACCGGAGAGCGATACGGCTTCAGTCAACGc < 1:3083885/69‑1 (MQ=255) aTCGCGGACCTGAACAATCGGGCCGGATGGAATGAACAGACCGGAGAGCGATACGGCTTCAGTCAACGc < 1:2880322/69‑1 (MQ=255) aTCGCGGACCTGAACAATCGGGCCGGATGGAATGAACAGACCGGAGAGCGATACGGCTTCAGTCAACGc < 1:3079815/69‑1 (MQ=255) tCGCGGACCTGAACAATCGGGCCGGATGGAATGAACAGACCGGAGAGCGATACGGCTTCAGTCAACGc < 1:3294870/68‑1 (MQ=255) cTGAACAATGGGACCCGCAGGAATAAACAGACCGGAGAGCGATACGGCTTCAGTTAACGCCCCACCGcc < 1:760826/69‑1 (MQ=255) tGGGACCCGCAGGAATAAACAGACCGGAGAGCGATACGGCTTCAGTTAACGc > 1:3341627/1‑52 (MQ=255) tGGGACCCGCAGGAATAAACAGACCGGAGAGCGATACGGCTTCAGTTAACGc > 1:224390/1‑52 (MQ=255) tGGGACCCGCAGGAATAAACAGACCGGAGAGCGATACGGCTTCAGTTAACGc > 1:3236948/1‑52 (MQ=255) tGGGACCCGCAGGAATAAACAGACCGGAGAGCGATACGGCTTCAGTTAACGc > 1:3197867/1‑52 (MQ=255) tGGGACCCGCAGGAATAAACAGACCGGAGAGCGATACGGCTTCAGTTAACGc > 1:3108223/1‑52 (MQ=255) tGGGACCCGCAGGAATAAACAGACCGGAGAGCGATACGGCTTCAGTTAACGc > 1:1314150/1‑52 (MQ=255) tGGGACCCGCAGGAATAAACAGACCGGAGAGCGATACGGCTTCAGTTAACGc > 1:916520/1‑52 (MQ=255) | TCCGTCGGTATCGCTATCTTCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATAAACAGACCGGAGAGCGATACGGCTTCAGTTAACGCCCCACCGCC > minE/1217431‑1217547 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |