| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 99,532 | T→G | 51.7% | Q311H (CAA→CAC) | hofC ← | assembly protein in type IV pilin biogenesis, transmembrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 99,532 | 0 | T | G | 51.7% | 13.2 / 62.9 | 62 | Q311H (CAA→CAC) | hofC | assembly protein in type IV pilin biogenesis, transmembrane protein |
| Reads supporting (aligned to +/- strand): ref base T (16/14); new base G (18/14); total (34/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGTTCTCACTAATTGCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGAAGTTGCACCCAGTACGGGCAGCG > minE/99465‑99591 | cTGTTCTCACTAATTGCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATGGGtt > 1:1998230/1‑69 (MQ=255) ctcACTAATTGCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGAcc > 1:1864966/1‑68 (MQ=255) cACTAATTTCAAACAGAGCGGGCTAAACTCACCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGt < 1:373994/68‑1 (MQ=255) cACTAATTGCAAACAGAGCGGGCTAAACTCACCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGt < 1:1959136/68‑1 (MQ=255) cACTAATTGCAAACAGAGCGGGCTAAACTCACCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGt < 1:873958/68‑1 (MQ=255) cACTAATTGCAAACAGAGCGGGCTAAACTCACCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGt < 1:3223972/68‑1 (MQ=255) cACTAATTGCAAACAGAGCGGGCTAAACTCACCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGt < 1:2246421/68‑1 (MQ=255) cACTAATTGCAAACAGAGCGGGCTAAACTCACCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGt < 1:2221395/68‑1 (MQ=255) cACTAATTGCAAACAGAGCGGGCTAAACTCACCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGt < 1:1317550/68‑1 (MQ=255) cACTAATTGCAAACAGAGCGGGCTAAACTCACCGGCATTTTTTAGCGCCAGCCAGATGGGGCGACCGt < 1:4119/68‑1 (MQ=255) aCTAATTGCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTa > 1:3176997/1‑69 (MQ=255) tGCAAACAGAGCGGGCTAAACTCACCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGata < 1:377610/69‑1 (MQ=255) tGCAAACAGAGCGGGCTAAACTCACCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGata < 1:1916012/69‑1 (MQ=255) tGCAAACAGAGCGGGCTAAACTCACCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGata < 1:1635903/69‑1 (MQ=255) tGCAAACAGAGCGGGCTAAACTCACCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGata < 1:454023/69‑1 (MQ=255) tGCAAACAGAGCGGGCTAAACTCACCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGata < 1:1201037/69‑1 (MQ=255) gCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGatat > 1:3322153/1‑69 (MQ=255) gCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGatat > 1:2351385/1‑69 (MQ=255) gCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGatat > 1:2418175/1‑69 (MQ=255) gCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGatat > 1:2981305/1‑69 (MQ=255) gCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGatat > 1:895724/1‑69 (MQ=255) gCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGatat > 1:1959870/1‑69 (MQ=255) gCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGatat > 1:188407/1‑69 (MQ=255) gCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGatat > 1:876783/1‑69 (MQ=255) gCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGatat > 1:806236/1‑69 (MQ=255) gCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGatat > 1:1183991/1‑69 (MQ=255) gCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGata > 1:3321966/1‑68 (MQ=255) gCAAACAGAGCGGGCTAAACTCACCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGata < 1:1706735/68‑1 (MQ=255) ggCTAAACTCACC‑GTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGATATCGTGCTGGATTtg < 1:2791519/69‑1 (MQ=255) gCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGATATCGTGCTGGATTtg < 1:2244418/69‑1 (MQ=255) gCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGATATCGTGCTGGATTtg < 1:767939/69‑1 (MQ=255) gCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGATATCGTGCTGGATTtg < 1:196537/69‑1 (MQ=255) gCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGATATCGTGCTGGATTtg < 1:1486001/69‑1 (MQ=255) gCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGATATCGTGCTGGATTtg < 1:1982867/69‑1 (MQ=255) aaaCTCACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGATATCGTGCTGGATTTgtgt < 1:2262858/69‑1 (MQ=255) cACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGaa > 1:1265049/1‑69 (MQ=255) aCCGGCATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGaa > 1:2149089/1‑68 (MQ=255) aCCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGaa > 1:497461/1‑68 (MQ=255) aCCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGaa > 1:3075169/1‑68 (MQ=255) aCCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGaa > 1:970722/1‑68 (MQ=255) aCCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGaa > 1:2631769/1‑68 (MQ=255) aCCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGaa > 1:2607989/1‑68 (MQ=255) aCCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGaa > 1:2464275/1‑68 (MQ=255) aCCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGaa > 1:2790698/1‑68 (MQ=255) aCCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGaa > 1:2348154/1‑68 (MQ=255) aCCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGaa > 1:211963/1‑68 (MQ=255) aCCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGaa > 1:2057736/1‑68 (MQ=255) aCCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGaa > 1:200294/1‑68 (MQ=255) aCCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGaa > 1:1988884/1‑68 (MQ=255) aCCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGaa > 1:1094611/1‑68 (MQ=255) aCCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGaa > 1:1153097/1‑68 (MQ=255) aCCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGaa > 1:1104205/1‑68 (MQ=255) aCCGGCATTTTTTAGCGCCAGCCAGATGGGGTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGa > 1:3187159/1‑67 (MQ=255) aCCGGCATTTTTTAGCCCCAGCCAGATGGGGTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGaa > 1:934087/1‑68 (MQ=255) aCCGGCATTTTTTAGCCCCAGCCAGATGGGGTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGaa > 1:930179/1‑68 (MQ=255) ttttttAGCGCCAGCCAGATGGGTTGACCGTTACTGat > 1:1013949/1‑38 (MQ=255) ttAGCGCCAGCCAGATGGGTTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGAAGTTGCACCCAg < 1:1643750/68‑1 (MQ=255) ccagATGGGTTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGAAGTTGCACCCAGTACGGGCAgcg < 1:792565/69‑1 (MQ=255) ccagATGGGTTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGAAGTTGCACCCAGTACGGGCAgcg < 1:1166313/69‑1 (MQ=255) ccagATGGGTTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGAAGTTGCACCCAGTACGGGCAgcg < 1:1072335/69‑1 (MQ=255) ccagATGGGTTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGAAGTTGCACCCAGTACGGGCAgcg < 1:102737/69‑1 (MQ=255) ccagATGGGTTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGAAGTTGCACCCAGTACGGGCAgcg < 1:1529144/69‑1 (MQ=255) ccagATGGGTTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGAAGTTGCACCCAGTACGGGCAgcg < 1:2401596/69‑1 (MQ=255) | CTGTTCTCACTAATTGCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGATATCGTGCTGGATTTGTGTCAGAAGTTGCACCCAGTACGGGCAGCG > minE/99465‑99591 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |