| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,220,477 | G→A | 70.6% | V102I (GTT→ATT) | yebS → | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,220,477 | 0 | G | A | 70.6% | 55.3 / 26.0 | 61 | V102I (GTT→ATT) | yebS | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (11/7); new base A (16/27); total (27/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.97e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TATGCCGTTTGCCTGGGGCGAACCGCTGTTGCATATCTGGCTGTTAGGCATTCGTATTGACGCCAACGTTATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACGGGGTCGATGGTCT > minE/1220410‑1220537 | tATGCCGTTTGCCTGGGGCGAACCGCTGTTGCATATCTGGCTGTTAGGCATTCGTATTGACGCCAACGt < 1:801167/69‑1 (MQ=255) tGCCTGGGGCGAACCGCTGTTGCATATCTGGCTGTTAGGCATTCGTATTGACGCCAACGTTATGCAAgg < 1:2022300/69‑1 (MQ=255) tGCCTGGGGCGAACCGCTGTTGCATATCTGGCTGTTAGGCATTCGTATTGACGCCAACGTTATGCAAgg < 1:1491987/69‑1 (MQ=255) tGCCTGGGGCGAACCGCTGTTGCATATCTGGCTGTTAGGCATTCGTATTGACGCCAACGTTATGCAAgg < 1:1941552/69‑1 (MQ=255) tGGGGCGAACCGCTGTTGCATATCTGGCTGTTAGGCATTCGTATTGACGCCAACATTATGCAAGGCAt > 1:792140/1‑68 (MQ=255) tGGGGCGAACCGCTGTTGCATATCTGGCTGTTAGGCATTCGTATTGACGCCAACATTATGCAAGGCATc > 1:2817242/1‑69 (MQ=255) tGGGGCGAACCGCTGTTGCATATCTGGCTGTTAGGCATTCGTATTGACGCCAACATTATGCAAGGCATc > 1:313846/1‑69 (MQ=255) tGGGGCGAACCGCTGTTGCATATCTGGCTGTTAGGCATTCGTATTGACGCCAACATTATGCAAGGCATc > 1:1106701/1‑69 (MQ=255) tGGGGCGAACCGCTGTTGCATATCTGGCTGTTAGGCATTCGTATTGACGCCAACATTATGCAAGGCATc > 1:3387810/1‑69 (MQ=255) tGGGGCGAACCGCTGTTGCATATCTGGCTGTTAGGCATTCGTATTGACGCCAACATTATGCAAGGCATc > 1:2012313/1‑69 (MQ=255) tGGGGCGAACCGCTGTTGCATATCTGGCTGTTAGGCATTCGTATTGACGCCAACATTATGCAAGGCATc > 1:257309/1‑69 (MQ=255) tGGGGCGAACCGCTGTTGCATATCTGGCTGTTAGGCATTCGTATTGACGCCAACATTATGCAAGGCATc > 1:1947029/1‑69 (MQ=255) tGGGGCGAACCGCTGTTGCATATCTGGCTGTTAGGCATTCGTATTGACGCCAACATTATGCAAGGCATc > 1:1884062/1‑69 (MQ=255) tGGGGCGAACCGCTGTTGCATATCTGGCTGTTAGGCATTCGTATTGACGCCAACATTATGCAAGGCATc > 1:665112/1‑69 (MQ=255) tGGGGCGAACCGCTGTTGCATATCTGGCTGTTAGGCATTCGTATTGACGCCAACATTATGCAAGGCATc > 1:2995030/1‑69 (MQ=255) tGGGGCGAACCGCTGTTGCATATCTGGCTGTTAGGCATTCGTATTGACGCCAACATTATGCAAGGCATc > 1:2991287/1‑69 (MQ=255) tGGGGCGAACCGCTGTTGCATATCTGGCTGTTAGGCATTCGTATTGACGCCAACATTATGCAAGGCATc > 1:1345037/1‑69 (MQ=255) tGGGGCGAACCGCTGTTGCATATCTGGCTGTTAGGCATTCGTATTGACGCCAACATTATGCAAGGCATc > 1:1252712/1‑69 (MQ=255) tGGGGCGAACCGCTGTTGCATATCTGGCTGTTAGGCATTCGTATTGACGCCAACATTATGCAAGGCATc > 1:1217557/1‑69 (MQ=255) tGGGGCGAACCGCTGTTGCATATCTGGCTGTTAGGCATTCGTATTGACGCCAACATTATGCAAGGCATc > 1:963208/1‑69 (MQ=255) ggggCGAACCGCTGTTGCATATCTGGCTGTTAGGCATTCGTATTGACGCCAACGTTATGCAAGGCATc > 1:504505/1‑68 (MQ=255) ggggCGAACCGCTGTTGCATATCTGGCTGTTAGGCATTCGTATTGACGCCAACGTTATGCAAGGCATCt > 1:1927114/1‑69 (MQ=255) ggggCGAACCGCTGTTGCATATCTGGCTGTTAGGCATTCGTATTGACGCCAACGTTATGCAAGGCATCt > 1:1139868/1‑69 (MQ=255) ggggCGAACCGCTGTTGCATATCTGGCTGTTAGGCATTCGTATTGACGCCAACGTTATGCAAGGCATCt > 1:2277823/1‑69 (MQ=255) aaCCGCTGTTGCATATCTGGCTTTTAGGCATTCGTATTGACGCCAACGTTATGCAAgg < 1:2319071/58‑1 (MQ=255) tatCTGGCTGTTAGGCATTCGTATTGACGCCAACGTTATGCAAGGCATCTGGCAAATGACCAAACAgg > 1:2250560/1‑68 (MQ=255) ggCATTCGTATTGACGCCAACATTATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACg < 1:273418/69‑1 (MQ=255) gCATTCGTATTGACGCCAACATTATGCAAGGCATCTGGCAAATGTCCAATCAGGGCGATGCGATAACg < 1:1133654/68‑1 (MQ=255) gCATTCGTATTGACGCCAACATTATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCTATAACg < 1:440185/68‑1 (MQ=255) gCATTCGTATTGACGCCAACATTATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACg < 1:3187654/68‑1 (MQ=255) gCATTCGTATTGACGCCAACATTATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACg < 1:3035842/68‑1 (MQ=255) gCATTCGTATTGACGCCAACATTATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACg < 1:3232877/68‑1 (MQ=255) gCATTCGTATTGACGCCAACATTATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACg < 1:705675/68‑1 (MQ=255) gCATTCGTATTGACGCCAACATTATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACg < 1:87958/68‑1 (MQ=255) gCATTCGTATTGACGCCAACATTATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACg < 1:2951186/68‑1 (MQ=255) gCATTCGTATTGACGCCAACATTATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACg < 1:2943803/68‑1 (MQ=255) gCATTCGTATTGACGCCAACATTATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACg < 1:2871833/68‑1 (MQ=255) gCATTCGTATTGACGCCAACATTATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACg < 1:964013/68‑1 (MQ=255) gCATTCGTATTGACGCCAACATTATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACg < 1:251673/68‑1 (MQ=255) gCATTCGTATTGACGCCAACATTATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACg < 1:2636687/68‑1 (MQ=255) gCATTCGTATTGACGCCAACATTATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACg < 1:1604655/68‑1 (MQ=255) gCATTCGTATTGACGCCAACATTATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACg < 1:1606499/68‑1 (MQ=255) gCATTCGTATTGACGCCAACATTATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACg < 1:1616875/68‑1 (MQ=255) gCATTCGTATTGACGCCAACATTATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACg < 1:1797509/68‑1 (MQ=255) gCATTCGTATTGACGCCAACATTATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACg < 1:2734089/68‑1 (MQ=255) gCATTCGTATTGACGCCAACATTATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACg < 1:1999826/68‑1 (MQ=255) gCATTCGTATTGACGCCAACATTATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACg < 1:2034918/68‑1 (MQ=255) gCATTCGTATTGACGCCAACATTATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACg < 1:2289293/68‑1 (MQ=255) gCATTCGTATTGACGCCAACATTATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACg < 1:1191554/68‑1 (MQ=255) gCATTCGTATTGACGCCAACATTATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACg < 1:2423105/68‑1 (MQ=255) gCATTCGTATTGACGCCAACATTATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACg < 1:1135916/68‑1 (MQ=255) gCATTCGTATTGACGCCAACATTATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACg < 1:2605203/68‑1 (MQ=255) gCATTCGTATTGACGCCAACATTATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACg < 1:262030/68‑1 (MQ=255) cGTATTGACGCCAACGTTATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACGGGGTCg > 1:898195/1‑69 (MQ=255) cGTATTGACGCCAACGTTATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACGGGGTCg > 1:1598953/1‑69 (MQ=255) cGTATTGACGCCAACGTTATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACGGGGTCg > 1:1964197/1‑69 (MQ=255) cGTATTGACGCCAACGTTATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACGGGGTCg > 1:736726/1‑69 (MQ=255) cGTATTGACGCCAACGTTATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACGGGGTCg > 1:2076277/1‑69 (MQ=255) cGTATTGACGCCAACGTTATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACGGGGTCg > 1:2420307/1‑69 (MQ=255) cGCCAACGTTATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACGGGGTCGATGGTCt < 1:2588462/68‑1 (MQ=255) cGCCAACGTTATGCAAGGCATCTGGCAAATGACCAAACAGGGCGAGGCGATAACGGGGTAGATGTTCt < 1:96828/68‑1 (MQ=255) | TATGCCGTTTGCCTGGGGCGAACCGCTGTTGCATATCTGGCTGTTAGGCATTCGTATTGACGCCAACGTTATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACGGGGTCGATGGTCT > minE/1220410‑1220537 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |