| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,220,863 | T→C | 22.8% | L230L (CTT→CTC) | yebS → | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,220,863 | 0 | T | C | 22.8% | 58.6 / 12.4 | 48 | L230L (CTT→CTC) | yebS | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (6/31); new base C (6/5); total (12/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.76e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCC > minE/1220808‑1220918 | aaCTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGAGGAGAAACTTCGTGTCTGTCTTg < 1:83185/69‑1 (MQ=255)aaCTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTg < 1:3333961/69‑1 (MQ=255)aaCTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTg < 1:2589416/69‑1 (MQ=255)aaCTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTg < 1:2639415/69‑1 (MQ=255)aaCTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTg < 1:3059848/69‑1 (MQ=255)aaCTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTg < 1:3415897/69‑1 (MQ=255)aaCTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTg < 1:1457502/69‑1 (MQ=255)aaCTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTg < 1:512064/69‑1 (MQ=255)aaCTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTg < 1:1306460/69‑1 (MQ=255)aaCTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTg < 1:1279082/69‑1 (MQ=255)aaCTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTg < 1:846284/69‑1 (MQ=255) gCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTg < 1:3003689/59‑1 (MQ=255) gCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGc > 1:2391073/1‑64 (MQ=255) gCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCAttt < 1:2048924/69‑1 (MQ=255) gCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCAttt < 1:2823988/69‑1 (MQ=255) gCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCAttt < 1:2255395/69‑1 (MQ=255) gCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTCTCTTGGGTGc > 1:403186/1‑64 (MQ=255) cGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCAtttt < 1:2813784/69‑1 (MQ=255) cGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCAtttt < 1:3087109/69‑1 (MQ=255) cGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCAtttt < 1:2498015/69‑1 (MQ=255) cGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCAtttt < 1:2505897/69‑1 (MQ=255) cGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCAtttt < 1:107209/69‑1 (MQ=255) cGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCAtttt < 1:2171003/69‑1 (MQ=255) aGCGCCCCGCTACGCGTAGGGACGAGAAACTCCGTGTCTGTCTTGGGTGCCATTTTACCGGc < 1:79393/62‑1 (MQ=255) aGCGCCCCGCTACGCGTAGGGACGAGAAACTCCGTGTCTGTCTTGGGTGCCATTTTACCGGc < 1:633530/62‑1 (MQ=255) aGCGCCCCGCTACGCGTAGGGACGAGAAACTCCGTGTCTGTCTTGGGTGCCATTTTACCGGc < 1:331318/62‑1 (MQ=255) aGCGCCCCGCTACGCGTAGGGACGAGAAACTCCGTGTCTGTCTTGGGTGCCATTTTACCGGc < 1:1513441/62‑1 (MQ=255) ccGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGc < 1:70785/69‑1 (MQ=255) ccGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGc < 1:2237380/69‑1 (MQ=255) ccGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGc < 1:697773/69‑1 (MQ=255) ccGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGc < 1:324349/69‑1 (MQ=255) gCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTg < 1:3133200/37‑1 (MQ=255) tACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTgg > 1:1974911/1‑69 (MQ=255) aCGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGt < 1:1071902/69‑1 (MQ=255) aCGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGt < 1:517663/69‑1 (MQ=255) aCGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGt < 1:2474917/69‑1 (MQ=255) aCGCGTAGGGACGAGAAACTCCGTGTCTGTCTTGGGTGCCATTTTAc > 1:1069114/1‑47 (MQ=255) aCGCGTAGGGACGAGAAACTCCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCTGa > 1:3413768/1‑59 (MQ=255) aCGCGTAGGGACGAGAAACTCCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCTGa > 1:3179657/1‑59 (MQ=255) aCGCGTAGGGACGAGAAACTCCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCTGa > 1:2157792/1‑59 (MQ=255) aCGCGTAGGGACGAGAAACTCCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCTGa > 1:769708/1‑59 (MQ=255) aCGCGTAGGGACGAGAAACTCCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCTGa > 1:2733046/1‑59 (MQ=255) cgcgTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGt < 1:2710328/68‑1 (MQ=255) gTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCAtttt < 1:146314/41‑1 (MQ=255) gggACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGcc > 1:1329143/1‑69 (MQ=255) gggACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGcc > 1:651424/1‑69 (MQ=255) gggACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGcc > 1:773898/1‑69 (MQ=255) cGAGAAACTCCGTGTCTGTCTTGGGTGCCATTTTACCGGc < 1:1864004/40‑1 (MQ=255) | AACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCC > minE/1220808‑1220918 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |