| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,221,439 | C→T | 37.4% | N422N (AAC→AAT) T5M (ACG→ATG) |
yebS → yebT → |
conserved inner membrane protein conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,221,439 | 0 | C | T | 37.4% | 43.0 / 42.5 | 72 | N422N (AAC→AAT) T5M (ACG→ATG) | yebS yebT | conserved inner membrane protein conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (20/25); new base T (4/23); total (24/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.09e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.66e-01 | |||||||||||
ATTTTGACTATTCTTGCTGTGGAATGGCTGGACAGCCGCTTACTTTGGGATGCACATGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCAGATTAAAAATAAACGCCGTATCTCACCTTT > minE/1221371‑1221493 | aTTTTGACTATTCTTGCTGTGGAATGGCTGGACAGCCGCTTACTTTGGGATGCACATGAGTCAGGAAAc > 1:3394272/1‑69 (MQ=255)aTTTTGACTATTCTTGCTGTGGAATGGCTGGACAGCCGCTTACTTTGGGATGCACATGAGTCAGGAAAc > 1:3108986/1‑69 (MQ=255)aTTTTGACTATTCTTGCTGTGGAATGGCTGGACAGCCGCTTACTTTGGGATGCACATGAGTCAGGAAAc > 1:300017/1‑69 (MQ=255) ttGACTATTCTTGCTGTGGAATGGCTGGACAGCCGCTTACTTTGGGATGCACATGAGTCAGGAAACGcc > 1:3376413/1‑69 (MQ=255) ttGACTATTCTTGCTGTGGAATGGCTGGACAGCCGCTTACTTTGGGATGCACATGAGTCAGGAAACGcc > 1:2610646/1‑69 (MQ=255) aTGGCTGGACAGCCGCTTACTTTGGGATGCACATGAGTCAGGAAACGCCCGCTTCGACGACTGAAgcg < 1:1007822/68‑1 (MQ=255) aTGGCTGGACAGCCGCTTACTTTGGGATGCACATGAGTCAGGAAACGCCCGCTTCGACGACTGAAgcg < 1:124066/68‑1 (MQ=255) aTGGCTGGACAGCCGCTTACTTTGGGATGCACATGAGTCAGGAAACGCCCGCTTCGACGACTGAAgcg < 1:1396912/68‑1 (MQ=255) aTGGCTGGACAGCCGCTTACTTTGGGATGCACATGAGGCAGGAAACGCCCGCTTCGACGACTGAAgcg < 1:2881540/68‑1 (MQ=255) tGGCTGGACAGCCGCTTACTTTGGGATGCACATGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCa < 1:778710/69‑1 (MQ=255) tGGCTGGACAGCCGCTTACTTTGGGATGCACATGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCa < 1:3223806/69‑1 (MQ=255) tGGCTGGACAGCCGCTTACTTTGGGATGCACATGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCa < 1:2150746/69‑1 (MQ=255) tGGCTGGACAGCCGCTTACTTTGGGATGCACATGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCa < 1:2958387/69‑1 (MQ=255) tGGCTGGACAGCCGCTTACTTTGGGATGCACATGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCa < 1:1685404/69‑1 (MQ=255) cAGCCGCTTACTTTGGGATGCACATGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCAGATTaaa < 1:2689536/68‑1 (MQ=255) cAGCCGCTTACTTTGGGATGCACATGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCAGATTaaa < 1:2252267/68‑1 (MQ=255) cAGCCGCTTACTTTGGGATGCACATGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCAGATTaaa < 1:3239290/68‑1 (MQ=255) cAGCCGCTTACTTTGGGATGCACATGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCAGATTaaa < 1:1756180/68‑1 (MQ=255) cAGCCGCTTACTTTGGGATGCACATGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCAGATTaaa < 1:183389/68‑1 (MQ=255) cAGCCGCTTACTTTGGGATGCACATGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCAGATTaaa < 1:1410448/68‑1 (MQ=255) cAGCCGCTTACTTTGGGATGCACATGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCAGATTaaa < 1:598245/68‑1 (MQ=255) cAGCCGCTTACTTTGGGATGCACATGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCAGATTaaa < 1:150412/68‑1 (MQ=255) cAGCCGCTTACTTTGGGATGCACATGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCAGATTaaa < 1:515479/68‑1 (MQ=255) cAGCCGCTTACTTTGGGATGCACATGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCAGATTaaa < 1:151445/68‑1 (MQ=255) ccGCTTACTTTGGGATGCACATGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCAGATTAAAAATa < 1:1199363/69‑1 (MQ=255) cGCTTACTTTGGGATGCACATGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCAGATTAAAAATaa > 1:1717181/1‑69 (MQ=255) cGCTTACTTTGGGATGCACATGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCAGATTAAAAATaa > 1:803362/1‑69 (MQ=255) cGCTTACTTTGGGATGCACATGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCAGATTAAAAATaa > 1:2752282/1‑69 (MQ=255) cGCTTACTTTGGGATGCACATGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCAGATTAAAAATaa > 1:252400/1‑69 (MQ=255) cGCTTACTTTGGGATGCACATGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCAGATTAAAAATaa > 1:3129555/1‑69 (MQ=255) cGCTTACTTTGGGATGCACATGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCAGATTAAAAATaa > 1:3166115/1‑69 (MQ=255) cGCTTACTTTGGGATGCACATGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCAGATTAAAAATaa > 1:2263602/1‑69 (MQ=255) cGCTTACTTTGGGATGCACATGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCAGATTAAAAATaa > 1:400211/1‑69 (MQ=255) cGCTTACTTTGGGATGCACATGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCAGATTAAAAATaa > 1:396295/1‑69 (MQ=255) cGCTTACTTTGGGATGCACATGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCAGATTAAAAATa < 1:2673349/68‑1 (MQ=255) cGCTTACTTAGGGATGCACATGAGTCAGCAAACGCCCGCTTCGACGACTGAAGCGCAGATTAAAAATa > 1:1653123/1‑68 (MQ=255) tACTTTGGGATGCACATGAGTCAGGATATGACCGCTTCGACGACTGAAGCGCAGATTaaaa < 1:2273487/61‑1 (MQ=255) tACTTTGGGATGCACATGAGTCAGGAAATGCCCGCTTCGACGACTGAAGCGCAGATTaaaa < 1:3209293/61‑1 (MQ=255) tACTTTGGGATGCACATGAGTCAGGAAATGCCCGCTTCGACGACTGAAGCGCAGATTaaaa < 1:3125429/61‑1 (MQ=255) tACTTTGGGATGCACATGAGTCAGGAAATGCCCGCTTCGACGACTGAAGCGCAGATTaaaa < 1:3256524/61‑1 (MQ=255) tACTTTGGGATGCACATGAGTCAGGAAATGCCCGCTTCGACGACTGAAGCGCAGATTaaaa < 1:3262128/61‑1 (MQ=255) tACTTTGGGATGCACATGAGTCAGGAAATGCCCGCTTCGACGACTGAAGCGCAGATTaaaa < 1:2539387/61‑1 (MQ=255) tACTTTGGGATGCACATGAGTCAGGAAATGCCCGCTTCGACGACTGAAGCGCAGATTaaaa < 1:392604/61‑1 (MQ=255) tACTTTGGGATGCACATGAGTCAGGAAATGCCCGCTTCGACGACTGAAGCGCAGATTaaaa < 1:800982/61‑1 (MQ=255) tACTTTGGGATGCACATGAGTCAGGAAATGCCCGCTTCGACGACTGAAGCGCAGATTaaaa < 1:37622/61‑1 (MQ=255) tACTTTGGGATGCACATGAGTCAGGAAATGCCCGCTTCGACGACTGAAGCGCAGATTaaaa < 1:2944655/61‑1 (MQ=255) tACTTTGGGATGCACATGAGTCAGGAAATGCCCGCTTCGACGACTGAAGCGCAGATTaaaa < 1:989177/61‑1 (MQ=255) tACTTTGGGATGCACATGAGTCAGGAAATGCCCGCTTCGACGACTGAAGCGCAGATTaaaa < 1:2871874/61‑1 (MQ=255) tACTTTGGGATGCACATGAGTCAGGAAATGCCCGCTTCGACGACTGAAGCGCAGATTaaaa < 1:1520924/61‑1 (MQ=255) tACTTTGGGATGCACATGAGTCAGGAAATGCCCGCTTCGACGACTGAAGCGCAGATTaaaa < 1:1560359/61‑1 (MQ=255) tACTTTGGGATGCACATGAGTCAGGAAATGCCCGCTTCGACGACTGAAGCGCAGATTaaaa < 1:1605075/61‑1 (MQ=255) tACTTTGGGATGCACATGAGTCAGGAAATGCCCGCTTCGACGACTGAAGCGCAGATTaaaa < 1:1198839/61‑1 (MQ=255) tACTTTGGGATGCACATGAGTCAGGAAATGCCCGCTTCGACGACTGAAGCGCAGATTaaaa < 1:1798636/61‑1 (MQ=255) tACTTTGGGATGCACATGAGTCAGGAAATGCCCGCTTCGACGACTGAAGCGCAGATTaaaa < 1:1909823/61‑1 (MQ=255) tACTTTGGGATGCACATGAGTCAGGAAATGCCCGCTTCGACGACTGAAGCGCAGATTaaaa < 1:2209810/61‑1 (MQ=255) tACTTTGGGATGCACATGAGTCAGGAAATGCCCGCTTCGACGACTGAAGCGCAGATTaaaa < 1:2375064/61‑1 (MQ=255) tACTATGGGATGCACATGAGTCAGGAAATGCCCGCTTCGACGACTGAAGCGCAGATTaaaa < 1:307113/61‑1 (MQ=255) aCTTTGGGATGCACATGAGTCAGGAAATGCCCGCTTCGACGACTGAAGCGCAGATTaaaa < 1:1391614/60‑1 (MQ=255) tGGGATGCACATGAGTCAGGAAATGCCCGCTTCGACTACTGAAGCGCAGATTAAAAATAAACGCCGTAt > 1:1583560/1‑69 (MQ=255) tGGGATGCACATGAGTCAGGAAATGCCCGCTTCGACGACTGAAGCGCAGATTAAAAATAAACGCCGTAt > 1:1961068/1‑69 (MQ=255) tGGGATGCACATGAGTCAGGAAATGCCCGCTTCGACGACTGAAGCGCAGATTAAAAATAAACGCCGTAt > 1:1141445/1‑69 (MQ=255) tGGGATGCACATGAGTCAGGAAATGCCCGCTTCGACGACTGAAGCGCAGATTAAAAATAAACGCCGTAt > 1:2467538/1‑69 (MQ=255) cacaTGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCAGATTAAAAATAAACGCCGTATCTCAcc > 1:3379367/1‑68 (MQ=255) cacaTGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCAGATTAAAAATAAACGCCGTATCTCAcc > 1:2602093/1‑68 (MQ=255) cacaTGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCAGATTAAAAATAAACGCCGTATCTCACCt > 1:2316056/1‑69 (MQ=255) cacaTGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCAGATTAAAAATAAACGCCGTATCTCACCt > 1:2060204/1‑69 (MQ=255) cacaTGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCAGATTAAAAATAAACGCCGTATCTCACCt > 1:1457815/1‑69 (MQ=255) caTGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCAGATTAAAAATAAACGCCGTATCTCACCttt > 1:2343638/1‑69 (MQ=255) caTGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCAGATTAAAAATAAACGCCGTATCTCACCttt > 1:1701221/1‑69 (MQ=255) caTGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCAGATTAAAAATAAACGCCGTATCTCACCttt > 1:814744/1‑69 (MQ=255) caTGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCAGATTAAAAATAAACGCCGTATCTCACCtt < 1:2764428/68‑1 (MQ=255) caTGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCAGATTAAAAATAAACGCCGTATCTCACCtt < 1:1676902/68‑1 (MQ=255) aTGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCAGATTAAAAATAAACGCCGTATCTCACCtt < 1:3376103/67‑1 (MQ=255) gAGTCAGGAAATGCCCGCTTCGACGACTGAAGCGCAGATTaaaa < 1:2570841/44‑1 (MQ=255) gTCAGCAAACGCCCGCTTCGACGACTGAAGCGCAGATTAAAAATAAACGCCGTATCTCACCtt < 1:1106761/63‑1 (MQ=255) | ATTTTGACTATTCTTGCTGTGGAATGGCTGGACAGCCGCTTACTTTGGGATGCACATGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCAGATTAAAAATAAACGCCGTATCTCACCTTT > minE/1221371‑1221493 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |