| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,221,569 | C→T | 44.1% | T48T (ACC→ACT) | yebT → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,221,569 | 0 | C | T | 44.1% | 16.7 / 51.2 | 68 | T48T (ACC→ACT) | yebT | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (13/25); new base T (14/16); total (27/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.11e-01 | |||||||||||
GCTAATGATTGCCAGTTGGCTGATTTGGGACAGTTATCAGGACCGGGGTAATACCGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCAGGCCGTACGCCTGTTCGTTATCAGGGCG > minE/1221515‑1221636 | gcTAATGATTGCCAGTTGGCTGATTTGGGACAGTTATCAGGACCGGGGTAATACCGTCACCATCGACtt < 1:2145361/69‑1 (MQ=255)gcTAATGATTGCCAGTTGGCTGATTTGGGACAGTTATCAGGACCGGGGTAATACCGTCACCATCGACtt < 1:11343/69‑1 (MQ=255)gcTAATGATTGCCAGTTGGCTGATTTGGGACAGTTATCAGGACCGGGGTAATACCGTCACCATCGACtt < 1:686042/69‑1 (MQ=255)gcTAATGATTGCCAGTTGGCTGATTTGGGACAGTTATCAGGACCGGGGTAATACCGTCACCATCGACtt < 1:232208/69‑1 (MQ=255)gcTAATGATTGCCAGTTGGCTGATTTGGGACAGTTATCAGGACCGGGGTAATACCGTCACCATCGACtt < 1:1785961/69‑1 (MQ=255)gcTAATGATTGCCAGTTGGCTGATTTGGGACAGTTATCAGGACCGGGGTAATACCGTCACCATCGACtt < 1:2749440/69‑1 (MQ=255)gcTAATGATTGCCAGTTGGCTGATTTGGGACAGTTATCAGGACCGGGGTAATACCGTCACCATCGACtt < 1:1541644/69‑1 (MQ=255)gcTAATGATTGCCAGTTGGCTGATTTGGGACAGTTATCAGGACCGGGGTAATACCGTCACCATCGACtt < 1:343060/69‑1 (MQ=255)gcTAATGATTGCCAGTTGGCTGATTTGGGACAGTTATCAGGACCGGGGTAATACCGTCACCATCGACtt < 1:2888328/69‑1 (MQ=255)gcTAATGATTGCCAGTTGCCTGATTTGGGACAGTTATCAGGACCGGGGTAATACCGTCACCATCGACtt < 1:655058/69‑1 (MQ=255) aaTGATTGCCAGTTGGCTGATTTGGGACAGTTATCAGGACCGGGGTAATACCGTCACCATCGACTTTAt < 1:1635145/69‑1 (MQ=255) aaTGATTGCCAGTTGGCTGATTTGGGACAGTTATCAGGACCGGGGTAATACCGTCACCATCGACTTTAt < 1:536672/69‑1 (MQ=255) tGCCAGTTGGCTGATTTGGGACAGTTATCAGGACCTGGGTAATACCGTCACCATCGACTTTATGTcgg < 1:906733/68‑1 (MQ=255) tGCCAGTTGGCTGATTTGGGACAGTTATCAGGACCGGGGTAATACCGTCACCATCGACTTTATGTcgg < 1:1484253/68‑1 (MQ=255) tGCCAGTTGGCTGATTTGGGACAGTTATCAGGACCGGGGTAATACCGTCACCATCGACTTTATGTcgg < 1:1381476/68‑1 (MQ=255) tGCCAGTTGGCTGATTTGGGACAGTTATCAGGACCGGGGTAATACCGTCACCATCGACTTTATGTcgg < 1:2621985/68‑1 (MQ=255) tGCCAGTTGGCTGATTTGGGACAGTTATCAGGACCGGGGTAATACCGTCACCATCGACTTTATGTcgg < 1:556834/68‑1 (MQ=255) tGCCAGTTGGCTGATTTGGGACAGTTATCAGGACCGGGGTAATACCGTCACCATCGACTTTATGTcgg < 1:1184318/68‑1 (MQ=255) tggTTGGCTGATTTGGGACAGTTATCAGGACCGGGGCAATACTGt > 1:562028/3‑45 (MQ=255) tggTTGGCTGATTTGGGACAGTTATCAGGACCGGGGCAATACTGTCACCATCGACTTTATGTCGGCGGa > 1:363911/3‑69 (MQ=255) tggTTGGCTGATTTGGGACAGTTATCAGGACCGGGGCAATACTGTCACCATCGACTTTATGTCGGCGGa > 1:106520/3‑69 (MQ=255) tggTTGGCTGATTTGGGACAGTTATCAGGACCGGGGCAATACTGTCACCATCGACTTTATGTCGGCGGa > 1:2543859/3‑69 (MQ=255) tggTTGGCTGATTTGGGACAGTTATCAGGACCGGGGCAATACTGTCACCATCGACTTTATGTCGGCGGa > 1:2500254/3‑69 (MQ=255) tggTTGGCTGATTTGGGACAGTTATCAGGACCGGGGCAATACTGTCACCATCGACTTTATGTCGGCGGa > 1:2462945/3‑69 (MQ=255) tggTTGGCTGATTTGGGACAGTTATCAGGACCGGGGCAATACTGTCACCATCGACTTTATGTCGGCGGa > 1:2615558/3‑69 (MQ=255) tggTTGGCTGATTTGGGACAGTTATCAGGACCGGGGCAATACTGTCACCATCGACTTTATGTCGGCGGa > 1:2078928/3‑69 (MQ=255) tggTTGGCTGATTTGGGACAGTTATCAGGACCGGGGCAATACTGTCACCATCGACTTTATGTCGGCGGa > 1:1167107/3‑69 (MQ=255) tggTTGGCTGATTTGGGACAGTTATCAGGACCGGGGCAATACTGTCACCATCGACTTTATGTCGGCGGa > 1:1462656/3‑69 (MQ=255) tggTTGGCTGATTTGGGACAGTTATCAGGACCGGGGCAATACTGTCACCATCGACTTTATGTCGGCGGa > 1:1517151/3‑69 (MQ=255) tggTTGGCTGATTTGGGACAGTTATCAGGACCGGGGCAATACTGTCACCATCGACTTTATGTCGGCGGa > 1:1617556/3‑69 (MQ=255) tggTTGGCTGATTTGGGACAGTTATCAGGACCGGGGCAATACTGTCACCATCGACTTTATGTCGGCGGa > 1:1201516/3‑69 (MQ=255) tggTTGGCTGATTTGGGACAGTTATCAGGACCGGGGCAATACTGTCACCATCGACTTTATGTCGGCGGa > 1:1647306/3‑69 (MQ=255) tGGGACAGTTATCAGGACCGGGGCAATACTGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCa < 1:2822596/69‑1 (MQ=255) tGGGACAGTTATCAGGACCGGGGCAATACTGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCa < 1:189609/69‑1 (MQ=255) tGGGACAGTTATCAGGACCGGGGCAATACTGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCa < 1:3387558/69‑1 (MQ=255) tGGGACAGTTATCAGGACCGGGGCAATACTGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCa < 1:1446983/69‑1 (MQ=255) tGGGACAGTTATCAGGACCGGGGCAATACTGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCa < 1:2835460/69‑1 (MQ=255) tGGGACAGTTATCAGGACCGGGGCAATACTGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCa < 1:122710/69‑1 (MQ=255) tGGGACAGTTATCAGGACCGGGGCAATACTGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCa < 1:2682592/69‑1 (MQ=255) tGGGACAGTTATCAGGACCGGGGCAATACTGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCa < 1:620326/69‑1 (MQ=255) tGGGACAGTTATCAGGACCGGGGCAATACTGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCa < 1:572178/69‑1 (MQ=255) tGGGACAGTTATCAGGACCGGGGCAATACTGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCa < 1:2194391/69‑1 (MQ=255) tGGGACAGTTATCAGGACCGGGGCAATACTGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCa < 1:1689325/69‑1 (MQ=255) tGGGACAGTTATCAGGACCGGGGCAATACTGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCa < 1:1747270/69‑1 (MQ=255) tGGGACAGTTATCAGGACCGGGGCAATACTGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCa < 1:242004/69‑1 (MQ=255) gggACTGTTATCAGGACCGGGGCAATACTGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCa < 1:411168/68‑1 (MQ=255) gggACAGTTATCAGGACCGGGGCAATACTGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCa < 1:1887445/68‑1 (MQ=255) gggACAGTTATCAGGACCGGGGCAATACTGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCa < 1:1919746/68‑1 (MQ=255) aaTACCGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCAGGCCGTACGCCTGTTCGTTATCAg < 1:479463/69‑1 (MQ=255) aaTACCGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCAGGCCGTACGCCTGTTCGTTATCAg < 1:3112778/69‑1 (MQ=255) aaTACCGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCAGGCCGTACGCCTGTTCGTTATCAg < 1:283803/69‑1 (MQ=255) aaTACCGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCAGGCCGTACGCCTGTTCGTTATCAg < 1:1075113/69‑1 (MQ=255) aaTACCGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCAGGCCGTACGCCTGTTCGTTATCAg < 1:1891539/69‑1 (MQ=255) aaTACCGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCAGGCCGTACGCCTGTTCGTTATCAg < 1:1563724/69‑1 (MQ=255) aaTACCGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCAGGCCGTACGCCTGTTCGTTATCAg < 1:3080317/69‑1 (MQ=255) aTACCGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCAGGCCGTACGCCTGTTCGTTATCAgg > 1:778263/1‑69 (MQ=255) aTACCGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCAGGCCGTACGCCTGTTCGTTATCAgg > 1:3304233/1‑69 (MQ=255) aCCGTCACCTTCGACTTTATGTCGGCGGATGGTATTGTTCCAGGCCGTACGCCTGTTCGTTATCAGGGc > 1:72790/1‑69 (MQ=255) aCCGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCAGGCCGTACGCCTGTTCGTTa > 1:136821/1‑62 (MQ=255) aCCGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCAGGCCGTACGCCTGTTCGTTATCAgggg > 1:1896011/1‑68 (MQ=255) aCCGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCAGGCCGTACGCCTGTTCGTTATCAGGGc > 1:36640/1‑69 (MQ=255) aCCGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCAGGCCGTACGCCTGTTCGTTATCAGGGc > 1:799184/1‑69 (MQ=255) aCCGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCAGGCCGTACGCCTGTTCGTTATCAGGGc > 1:3202548/1‑69 (MQ=255) aCCGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCAGGCCGTACGCCTGTTCGTTATCAGGGc > 1:516737/1‑69 (MQ=255) aCCGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCAGGCCGTACGCCTGTTCGTTATCAGGGc > 1:1619657/1‑69 (MQ=255) aCCGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCAGGCCGTACGCCTGTTCGTTATCAGGGc > 1:564146/1‑69 (MQ=255) aCCGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCAGGCCGTACGCCTGTTCGTTATCAGGGc > 1:314249/1‑69 (MQ=255) aCCGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCAGGCCGTACGCCTGTTCGTTATCAGGGc > 1:726661/1‑69 (MQ=255) ccGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCAGGCCGTACGCCTGTTCGTTATCAGGGc > 1:3271301/1‑68 (MQ=255) ccGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCAGGCCGTACGCCTGTTCGTTATCAGGGc > 1:396618/1‑68 (MQ=255) ccGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCAGGCCGTACGCCTGTTCGTTATCAGGGCg > 1:116913/1‑69 (MQ=255) ccGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCAGGCCGTACGCCTGTTCGTTATCAGGGCg > 1:3400993/1‑69 (MQ=255) ccGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCAGGCCGTACGCCTGTTCGTTATCAGGGCg > 1:2889666/1‑69 (MQ=255) ccGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCAGGCCGTACGCCTGTTCGTTATCAGGGCg > 1:1903233/1‑69 (MQ=255) | GCTAATGATTGCCAGTTGGCTGATTTGGGACAGTTATCAGGACCGGGGTAATACCGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCAGGCCGTACGCCTGTTCGTTATCAGGGCG > minE/1221515‑1221636 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |