| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,222,865 | G→A | 42.0% | G480G (GGG→GGA) | yebT → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,222,865 | 0 | G | A | 42.0% | 24.5 / 48.8 | 69 | G480G (GGG→GGA) | yebT | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (13/27); new base A (14/15); total (27/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.59e-01 | |||||||||||
GTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTAT > minE/1222800‑1222919 | gtgAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATa > 1:3053920/1‑69 (MQ=255)gtgAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATa > 1:127789/1‑69 (MQ=255)gtgAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATa > 1:2898727/1‑69 (MQ=255) aaGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACg < 1:2310408/68‑1 (MQ=255) gTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTAtt > 1:1623469/1‑69 (MQ=255) gTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATaaa < 1:3374479/69‑1 (MQ=255) gTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATaaa < 1:2264613/69‑1 (MQ=255) gTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATaaa < 1:625599/69‑1 (MQ=255) gTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATaaa < 1:704830/69‑1 (MQ=255) gTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATaaa < 1:813976/69‑1 (MQ=255) tttCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTgag > 1:2124943/1‑69 (MQ=255) tttCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTgag > 1:1692588/1‑69 (MQ=255) tttCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTga > 1:2008975/1‑68 (MQ=255) gTGCCAGCGCCTCAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGaaa > 1:1441117/1‑68 (MQ=255) gTGCCAGCGCCTCAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGaaa > 1:1075892/1‑68 (MQ=255) gTGCCAGCGCCTCAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGaaa > 1:1170015/1‑68 (MQ=255) gTGCCAGCGCCTCAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGaaa > 1:1905203/1‑68 (MQ=255) gTGCCAGCGCCTCAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGaaa > 1:2393636/1‑68 (MQ=255) gTGCCAGCGCCTCAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGaaa > 1:2988393/1‑68 (MQ=255) gTGCCAGCGCCTCAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGaaa > 1:2619194/1‑68 (MQ=255) gTGCCAGCGCCTCAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGaaa > 1:732136/1‑68 (MQ=255) gTGCCAGCGCCTCAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGaaa > 1:2927065/1‑68 (MQ=255) gTGCCAGCGCCTCAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGaaa > 1:804513/1‑68 (MQ=255) gTGCCAGCGCCTCAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGaaa > 1:157121/1‑68 (MQ=255) gTGCCAGCGCCTCAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGaaa > 1:3327919/1‑68 (MQ=255) gTGCCAGCGCCTCAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGaaa > 1:2912283/1‑68 (MQ=255) gTGCCAGCGCCTCAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGaaa > 1:144889/1‑68 (MQ=255) aGCGCCTCAGAATGGATCAATGGCGGGATACGTAt > 1:1938027/1‑35 (MQ=255) aGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGc > 1:1289915/1‑69 (MQ=255) aGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGc > 1:388387/1‑69 (MQ=255) aGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGc > 1:2502480/1‑69 (MQ=255) cataaTGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGAAAGCCa < 1:1060533/57‑1 (MQ=255) cAGAATGGATCAATGGCGGAATACGTATTCTGCCGGTCGATAAAGGCGAGATGAAAGCCa < 1:1990151/60‑1 (MQ=255) cAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGAAAGCCa < 1:2014779/60‑1 (MQ=255) cAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGAAAGCCa < 1:2992223/60‑1 (MQ=255) cAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGAAAGCCa < 1:1553681/60‑1 (MQ=255) cAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGAAAGCCa < 1:2418095/60‑1 (MQ=255) cAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGAAAGCCa < 1:1442937/60‑1 (MQ=255) cAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGAAAGCCa < 1:3319001/60‑1 (MQ=255) cAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGAAAGCCa < 1:3182979/60‑1 (MQ=255) cAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGAAAGCCa < 1:2971013/60‑1 (MQ=255) cAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGAAAGCCa < 1:1340909/60‑1 (MQ=255) cAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGAAAGCCa < 1:1307341/60‑1 (MQ=255) cAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAACGCGAGATGAAAGCCa < 1:122040/60‑1 (MQ=255) cAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCCATAAAGGCGAGATGAAAGCCa < 1:2620653/60‑1 (MQ=255) aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAGGCCAGCTATCCACTg < 1:1398691/68‑1 (MQ=255) aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:992538/68‑1 (MQ=255) aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:3390389/68‑1 (MQ=255) aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:308074/68‑1 (MQ=255) aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:844736/68‑1 (MQ=255) aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:587139/68‑1 (MQ=255) aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:2257479/68‑1 (MQ=255) aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:556369/68‑1 (MQ=255) aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:2954673/68‑1 (MQ=255) aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:2821099/68‑1 (MQ=255) aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:2621188/68‑1 (MQ=255) aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:2129709/68‑1 (MQ=255) aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:2100379/68‑1 (MQ=255) aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:2053302/68‑1 (MQ=255) aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:1897147/68‑1 (MQ=255) aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:1685291/68‑1 (MQ=255) aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:1574888/68‑1 (MQ=255) aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:1547393/68‑1 (MQ=255) aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:1396870/68‑1 (MQ=255) aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:1075718/68‑1 (MQ=255) tGGATCAATGGCGGGATACGTATTCTGCCGGGCGATaaa < 1:2400822/39‑1 (MQ=255) ggATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTAt > 1:1759572/1‑68 (MQ=255) cAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGAAAGCCa < 1:2158091/50‑1 (MQ=39) tGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCAAGCTATCCACTGTAt > 1:1865580/1‑61 (MQ=255) | GTGAAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTAT > minE/1222800‑1222919 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |