| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,222,892 | T→C | 43.5% | G489G (GGT→GGC) | yebT → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,222,892 | 0 | T | C | 43.5% | 15.9 / 66.7 | 85 | G489G (GGT→GGC) | yebT | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (12/36); new base C (14/23); total (26/59) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.40e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.53e-01 | |||||||||||
TTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTT > minE/1222827‑1222952 | tttCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTgag > 1:2124943/1‑69 (MQ=255)tttCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTgag > 1:1692588/1‑69 (MQ=255)tttCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTga > 1:2008975/1‑68 (MQ=255) gTGCCAGCGCCTCAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGaaa > 1:3327919/1‑68 (MQ=255) gTGCCAGCGCCTCAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGaaa > 1:2619194/1‑68 (MQ=255) gTGCCAGCGCCTCAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGaaa > 1:2393636/1‑68 (MQ=255) gTGCCAGCGCCTCAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGaaa > 1:2912283/1‑68 (MQ=255) gTGCCAGCGCCTCAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGaaa > 1:2927065/1‑68 (MQ=255) gTGCCAGCGCCTCAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGaaa > 1:2988393/1‑68 (MQ=255) gTGCCAGCGCCTCAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGaaa > 1:1905203/1‑68 (MQ=255) gTGCCAGCGCCTCAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGaaa > 1:157121/1‑68 (MQ=255) gTGCCAGCGCCTCAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGaaa > 1:144889/1‑68 (MQ=255) gTGCCAGCGCCTCAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGaaa > 1:1441117/1‑68 (MQ=255) gTGCCAGCGCCTCAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGaaa > 1:732136/1‑68 (MQ=255) gTGCCAGCGCCTCAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGaaa > 1:1075892/1‑68 (MQ=255) gTGCCAGCGCCTCAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGaaa > 1:1170015/1‑68 (MQ=255) gTGCCAGCGCCTCAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGaaa > 1:804513/1‑68 (MQ=255) aGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGc > 1:2502480/1‑69 (MQ=255) aGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGc > 1:388387/1‑69 (MQ=255) aGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGc > 1:1289915/1‑69 (MQ=255) cataaTGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGAAAGCCa < 1:1060533/57‑1 (MQ=255) cAGAATGGATCAATGGCGGAATACGTATTCTGCCGGTCGATAAAGGCGAGATGAAAGCCa < 1:1990151/60‑1 (MQ=255) cAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGAAAGCCa < 1:1307341/60‑1 (MQ=255) cAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGAAAGCCa < 1:1442937/60‑1 (MQ=255) cAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGAAAGCCa < 1:2418095/60‑1 (MQ=255) cAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGAAAGCCa < 1:1553681/60‑1 (MQ=255) cAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGAAAGCCa < 1:2971013/60‑1 (MQ=255) cAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGAAAGCCa < 1:2014779/60‑1 (MQ=255) cAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGAAAGCCa < 1:1340909/60‑1 (MQ=255) cAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGAAAGCCa < 1:2992223/60‑1 (MQ=255) cAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGAAAGCCa < 1:3182979/60‑1 (MQ=255) cAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGAAAGCCa < 1:3319001/60‑1 (MQ=255) cAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAACGCGAGATGAAAGCCa < 1:122040/60‑1 (MQ=255) cAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCCATAAAGGCGAGATGAAAGCCa < 1:2620653/60‑1 (MQ=255) aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAGGCCAGCTATCCACTg < 1:1398691/68‑1 (MQ=255) aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:3390389/68‑1 (MQ=255) aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:308074/68‑1 (MQ=255) aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:2954673/68‑1 (MQ=255) aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:844736/68‑1 (MQ=255) aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:992538/68‑1 (MQ=255) aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:2821099/68‑1 (MQ=255) aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:2621188/68‑1 (MQ=255) aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:556369/68‑1 (MQ=255) aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:1075718/68‑1 (MQ=255) aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:587139/68‑1 (MQ=255) aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:1897147/68‑1 (MQ=255) aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:1574888/68‑1 (MQ=255) aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:1685291/68‑1 (MQ=255) aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:1547393/68‑1 (MQ=255) aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:1396870/68‑1 (MQ=255) aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:2053302/68‑1 (MQ=255) aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:2100379/68‑1 (MQ=255) aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:2129709/68‑1 (MQ=255) aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:2257479/68‑1 (MQ=255) ggATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTAt > 1:1759572/1‑68 (MQ=255) cAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGAAAGCCa < 1:2158091/50‑1 (MQ=39) tGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCAAGCTATCCACTGTAt > 1:1865580/1‑61 (MQ=255) tATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAgcgc > 1:1275555/1‑68 (MQ=255) tATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAgcgc > 1:1835017/1‑68 (MQ=255) cTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGgag < 1:3169455/69‑1 (MQ=255) cTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGgag > 1:1341745/1‑69 (MQ=255) cTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGgag > 1:822310/1‑69 (MQ=255) tGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGgag < 1:1154799/68‑1 (MQ=255) gggCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAAc < 1:744312/67‑1 (MQ=255) gggCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAAc < 1:746964/67‑1 (MQ=255) gggCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAAc < 1:997172/67‑1 (MQ=255) gggCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAAc < 1:964728/67‑1 (MQ=255) gggCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAAc < 1:494278/67‑1 (MQ=255) gggCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAAc < 1:461018/67‑1 (MQ=255) gggCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAAc < 1:2693277/67‑1 (MQ=255) gggCGATAAAGGCGAGATGAAAGCCAGCTATCCACTGTATGCCAATCAGGAAAAAGCGCTgg < 1:3414160/62‑1 (MQ=255) gggCGATAAAGGCGAGATGAAAGCCAGCTATCCACTGTATGCCAATCAGGAAAAAGCGCTgg < 1:337841/62‑1 (MQ=255) gggCGATAAAGGCGAGATGAAAGCCAGCTATCCACTGTATGCCAATCAGGAAAAAGCGCTgg < 1:2895222/62‑1 (MQ=255) gggCGATAAAGGCGAGATGAAAGCCAGCTATCCACTGTATGCCAATCAGGAAAAAGCGCTgg < 1:2762083/62‑1 (MQ=255) gggCGATAAAGGCGAGATGAAAGCCAGCTATCCACTGTATGCCAATCAGGAAAAAGCGCTgg < 1:1164580/62‑1 (MQ=255) gggCGATAAAGGCGAGATGAAAGCCAGCTATCCACTGTATGCCAATCAGGAAAAAGCGCTgg < 1:912731/62‑1 (MQ=255) gggCGATAAAGGCGAGATGAAAGCCAGCTATCCACTGTATGCCAATCAGGAAAAAGCGCTgg < 1:2321169/62‑1 (MQ=255) ggCGATATAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGc < 1:3329795/69‑1 (MQ=255) ggCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGc < 1:414700/69‑1 (MQ=255) ggCGATAAAGGCGAGATGAAAGCCAGCTATCCACTGTATGCCAATCAGGAAAAAGCGCTgg < 1:807216/61‑1 (MQ=255) gATAAAGGTGATATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACATCCtt < 1:2311612/69‑1 (MQ=255) gATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCtt < 1:3412889/69‑1 (MQ=255) gATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCtt < 1:1691657/69‑1 (MQ=255) gATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCtt < 1:2238134/69‑1 (MQ=255) gATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCtt < 1:2866755/69‑1 (MQ=255) | TTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTT > minE/1222827‑1222952 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |