| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,225,149 | T→C | 54.6% | A337A (GCT→GCC) | yebU → | predicted methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,225,149 | 0 | T | C | 54.6% | 4.8 / 33.2 | 44 | A337A (GCT→GCC) | yebU | predicted methyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (11/9); new base C (11/13); total (22/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01 | |||||||||||
CCCCAAATACAAAGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGC > minE/1225095‑1225204 | cccaAAATACAAAGTGGGCAATTTCCCGTTTAGCCCGGTTAAAGATCGCGAAGCCGGTCAAATTCGTCa < 1:1433232/69‑1 (MQ=255)cccaAAATACAAAGTGGGCAATTTCCCGTTTAGCCCGGTGAAAGATCGCGAAGCCGGTCAAATTCGTCa < 1:2915736/69‑1 (MQ=255)cccaAAATACAAAGTGGGCAATTTCCCGTTTAGCCCGGTGAAAGATCGCGAAGCCGGTCAAATTCGTCa < 1:2892243/69‑1 (MQ=255)cccaAAATACAAAGTGGGCAATTTCCCGTTTAGCCCGGTGAAAGATCGCGAAGCCGGTCAAATTCGTCa < 1:2877694/69‑1 (MQ=255)cccaAAATACAAAGTGGGCAATTTCCCGTTTAGCCCGGTGAAAGATCGCGAAGCCGGTCAAATTCGTCa < 1:1210863/69‑1 (MQ=255)cccaAAATACAAAGTGGGCAATTTCCCGTTTAGCCCGGTGAAAGATCGCGAAGCCGGTCAAATTCGTCa < 1:1326113/69‑1 (MQ=255)cccaAAATACAAAGTGGGCAATTTCCCGTTTAGCCCGGTGAAAGATCGCGAAGCCGGTCAAATTCGTCa < 1:1405204/69‑1 (MQ=255)cccaAAATACAAAGTGGGCAATTTCCCGTTTAGCCCGGTGAAAGATCGCGAAGCCGGTCAAATTCGTCa < 1:280742/69‑1 (MQ=255)cccaAAATACAAAGTGGGCAATTTCCCGTTTAGCCCGGTGAAAGATCGCGAAGCCGGTCAAATTCGTCa < 1:2718581/69‑1 (MQ=255)cccaAAATACAAAGTGGGCAATTTCCCGTTTAGCCCGGTGAAAGATCGCGAAGCCGGTCAAATTCGTCa < 1:2574778/69‑1 (MQ=255)cccaAAATACAAAGTGGGCAATTTCCCGTTTAGCCCGGTGAAAGATCGCGAAGCCGGTCAAATTCGTCa < 1:367174/69‑1 (MQ=255)cccaAAATACAAAGTGGGCAATTTCCCGTTTAGCCCGGTGAAAGATCGCGAAGCCGGTCAAATTCGTCa < 1:2290201/69‑1 (MQ=255)cccaAAATACAAAGTGGGCAATTTCCCGTTTAGCCCGGTGAAAGATCGCGAAGCCGGTCAAATTCGTCa < 1:374922/69‑1 (MQ=255) aaGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACa < 1:28168/68‑1 (MQ=255) aaGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACa < 1:2521756/68‑1 (MQ=255) gTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGgt < 1:660482/69‑1 (MQ=255) tCGGTAATTTTCCGTTCAGCCCTGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGgtg < 1:1165110/69‑1 (MQ=255) tCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGgtg < 1:1915852/69‑1 (MQ=255) tCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGgtg < 1:2592483/69‑1 (MQ=255) aaTTTTCCGTTCAGCCCGGTGAAAGAACGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTgg > 1:2437888/1‑68 (MQ=255) aaTTTTCCGTTCAGCCCGGTGAAAGAACGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTgg > 1:791233/1‑68 (MQ=255) aaTTTTCCGTTCAGCCCGGTGAAAGAACGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTgg > 1:3209771/1‑68 (MQ=255) aaTTTTCCGTTCAGCCCGGTGAAAGAACGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTgg > 1:1071348/1‑68 (MQ=255) aaTATTCCGTTCAGCCAGGTGAAAGAACGCGAAGCTGGACAAATTCGTCAGGCGGCTAAAGGTGTTgg > 1:1638171/1‑68 (MQ=255) tttcCCGTTTAGCCCGGTGAAAGATCGCGAAGCCGGTCAAATTCGTCAGGCGGCTGCAAGTGTTg > 1:985518/1‑65 (MQ=255) tttcCCGTTTAGCCCGGTGAAAGATCGCGAAGCCGGTCAAATTCGTCAGGCGGCTGCAAGTGTTg > 1:744287/1‑65 (MQ=255) tttcCCGTTTAGCCCGGTGAAAGATCGCGAAGCCGGTCAAATTCGTCAGGCGGCTGCAAGTGTTg > 1:3419487/1‑65 (MQ=255) tttcCCGTTTAGCCCGGTGAAAGATCGCGAAGCCGGTCAAATTCGTCAGGCGGCTGCAAGTGTTg > 1:840729/1‑65 (MQ=255) tttcCCGTTTAGCCCGGTGAAAGATCGCGAAGCCGGTCAAATTCGTCAGGCGGCTGCAAGTGTTg > 1:878697/1‑65 (MQ=255) tttcCCGTTTAGCCCGGTGAAAGATCGCGAAGCCGGTCAAATTCGTCAGGCGGCTGCAAGTGTTg > 1:913334/1‑65 (MQ=255) tttcCCGTTTAGCCCGGTGAAAGATCGCGAAGCCGGTCAAATTCGTCAGGCGGCTGCAAGTGTTg > 1:102977/1‑65 (MQ=255) tttcCCGTTTAGCCCGGTGAAAGATCGCGAAGCCGGTCAAATTCGTCAGGCGGCTGCAAGTGTTg > 1:264233/1‑65 (MQ=255) tttcCCGTTTAGCCCGGTGAAAGATCGCGAAGCCGGTCAAATTCGTCAGGCGGCTGCAAGTGTTg > 1:2546941/1‑65 (MQ=255) tttcCCGTTTAGCCCGGTGAAAGATCGCGAAGCCGGTCAAATTCGTCAGGCGGCTGCAAGTGTTg > 1:2309988/1‑65 (MQ=255) tttcCCGTTTAGCCCGGTGAAAGATCGCGAAGCCGGTCAAATTCGTCAGGCGGCTGCAAGTGTTg > 1:2067294/1‑65 (MQ=255) cGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGaaa < 1:1032347/69‑1 (MQ=255) gAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAAcc > 1:3328435/1‑68 (MQ=255) gAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCt > 1:1650519/1‑69 (MQ=255) gAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCt > 1:970882/1‑69 (MQ=255) gAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTAAAGGTGTTGGCTTAAACTGGGATGAAAACCt > 1:203490/1‑69 (MQ=255) aaaGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAgg > 1:2043127/1‑41 (MQ=255) aaaGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAgg > 1:1700362/1‑41 (MQ=255) aGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTgc < 1:1411220/68‑1 (MQ=255) aGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTgc < 1:1105498/68‑1 (MQ=255) | CCCCAAATACAAAGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGC > minE/1225095‑1225204 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |