| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,238,257 | T→C | 28.5% | P88P (CCA→CCG) | edd ← | 6‑phosphogluconate dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,238,257 | 0 | T | C | 28.5% | 121.5 / 52.8 | 119 | P88P (CCA→CCG) | edd | 6‑phosphogluconate dehydratase |
| Reads supporting (aligned to +/- strand): ref base T (20/65); new base C (6/28); total (26/93) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01 | |||||||||||
Only 100 of 119 total aligned reads displayed.
GAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGTGAT > minE/1238196‑1238310 | gAACACCGCCCGCATCCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAgt < 1:1270605/69‑1 (MQ=255)gAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAgt < 1:1962003/69‑1 (MQ=255)gAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAgt < 1:2802441/69‑1 (MQ=255)gAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAgt < 1:2080477/69‑1 (MQ=255)gAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAgt < 1:41937/69‑1 (MQ=255)gAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAgt < 1:505743/69‑1 (MQ=255)gAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAgt < 1:2007189/69‑1 (MQ=255)gAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAgt < 1:1982349/69‑1 (MQ=255)gAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAgt < 1:633320/69‑1 (MQ=255)gAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAgt < 1:1732077/69‑1 (MQ=255)gAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAgt < 1:109890/69‑1 (MQ=255)gAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAgt < 1:1005366/69‑1 (MQ=255)gAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAgt < 1:1304922/69‑1 (MQ=255) aaCACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCATGGCTTTACGAATGATTTCTGGATAgt < 1:1870584/68‑1 (MQ=255) cacCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAGTGtt < 1:1186601/69‑1 (MQ=255) cacCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAGTGtt < 1:886811/69‑1 (MQ=255) cacCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAGTGtt < 1:2103653/69‑1 (MQ=255) cacCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAGTGtt < 1:1940343/69‑1 (MQ=255) cacCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAGTGtt < 1:1847129/69‑1 (MQ=255) cacCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAGTGtt < 1:2307459/69‑1 (MQ=255) cacCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAGTGtt < 1:2495887/69‑1 (MQ=255) cacCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAGTGtt < 1:1066527/69‑1 (MQ=255) acCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAGTGtt < 1:1613387/68‑1 (MQ=255) ccgcccgcAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAGTGtt < 1:1493239/67‑1 (MQ=255) cAACCGTATTCGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGcgcg < 1:576685/68‑1 (MQ=255) cAACCGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGcgcg < 1:1749767/68‑1 (MQ=255) cAACCGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGcgcg < 1:573811/68‑1 (MQ=255) cAACCGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGcgcg < 1:2537151/68‑1 (MQ=255) ccGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgag > 1:2017993/1‑68 (MQ=255) ccGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgag > 1:2116271/1‑68 (MQ=255) ccGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgag > 1:1972625/1‑68 (MQ=255) ccGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgag > 1:757714/1‑68 (MQ=255) ccGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgag > 1:198522/1‑68 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGagag < 1:13539/69‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGagag < 1:1738456/69‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGagag < 1:2495447/69‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:3141759/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:2850736/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:2852712/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:2907323/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:2911618/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:821472/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:2960656/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:780225/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:2992245/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:3339844/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:3373976/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:3422119/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:919740/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:878674/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:918180/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:865120/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:270508/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:1484821/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:2221869/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:144446/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:2324371/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:2344713/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:1136545/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:1332148/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATACTGTTCATAAGGCTGGTGAGCGgaga < 1:3276460/68‑1 (MQ=255) gCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:905957/67‑1 (MQ=255) cGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTc > 1:3142839/1‑69 (MQ=255) ttCATGCAGGGCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGt < 1:846842/68‑1 (MQ=255) ttCATGCAGGGCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGt < 1:387885/68‑1 (MQ=255) ttCATGCAGGGCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGt < 1:3022383/68‑1 (MQ=255) ttCATGCAGGGCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGt < 1:1837220/68‑1 (MQ=255) ttCATGCAGGGCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGt < 1:2908377/68‑1 (MQ=255) tCATGCAGGGCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGTta < 1:194145/69‑1 (MQ=255) gCAGGGCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATagg < 1:481628/69‑1 (MQ=255) gggCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATaggag < 1:670588/68‑1 (MQ=255) ggCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGt < 1:3196150/69‑1 (MQ=255) ggCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGt < 1:202350/69‑1 (MQ=255) ggCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGt < 1:3235105/69‑1 (MQ=255) ggCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGt < 1:2545786/69‑1 (MQ=255) ggCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGt < 1:2629456/69‑1 (MQ=255) ggCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGt < 1:2673192/69‑1 (MQ=255) ggCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGt < 1:2768684/69‑1 (MQ=255) tttACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGtgat < 1:3232347/69‑1 (MQ=255) tttACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGtgat < 1:1944139/69‑1 (MQ=255) tttACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGtgat < 1:437516/69‑1 (MQ=255) tttACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGtgat < 1:321781/69‑1 (MQ=255) tttACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGtgat < 1:1105420/69‑1 (MQ=255) ttACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGtgat < 1:1581279/68‑1 (MQ=255) tGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGc > 1:2077037/1‑41 (MQ=255) tGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGt > 1:1086388/1‑45 (MQ=255) tGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGt > 1:860953/1‑45 (MQ=255) tGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGt > 1:1111754/1‑45 (MQ=255) tGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGt > 1:76839/1‑45 (MQ=255) tGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGt > 1:638834/1‑45 (MQ=255) tGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGt > 1:487158/1‑45 (MQ=255) tGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGt > 1:339279/1‑45 (MQ=255) tGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGt > 1:2444151/1‑45 (MQ=255) tGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGt > 1:3030286/1‑45 (MQ=255) tGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGt > 1:2447456/1‑45 (MQ=255) tGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGt > 1:2965127/1‑45 (MQ=255) tGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGt > 1:2496521/1‑45 (MQ=255) tGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGt > 1:2584415/1‑45 (MQ=255) tGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGt > 1:2620720/1‑45 (MQ=255) tGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGt > 1:2766285/1‑45 (MQ=255) | GAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGTGAT > minE/1238196‑1238310 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |