| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 100,925 | C→G | 55.8% | A305A (GCG→GCC) | hofB ← | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 100,925 | 0 | C | G | 55.8% | 11.8 / 55.7 | 52 | A305A (GCG→GCC) | hofB | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| Reads supporting (aligned to +/- strand): ref base C (7/16); new base G (14/15); total (21/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACGTACCAGCGTTTCGCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCAGTTTGCGCCGCTTTAATAGCGATCTCTGCTGTTTCGCCATCGCGGATCTCTCCGATCATGATGACGTCAGGAT > minE/100858‑100989 | aaCGTACCAGCGTTTCGCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCAGTTTGcgccg > 1:3198966/1‑69 (MQ=255)aaCGTACCAGCGTTTCGCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCAGTTTGcgccg > 1:2568892/1‑69 (MQ=255)aaCGTACCAGCGTTTCGCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCAGTTTGcgccg > 1:331217/1‑69 (MQ=255)aaCGTACCAGCGTTTCGCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCAGTTTGcgccg > 1:1433344/1‑69 (MQ=255)aaCGTACCAGCGTTTCGCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCAGTTTGcgccg > 1:345757/1‑69 (MQ=255)aaCGTACCAGCGTTTCGCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCAGTTTGcgccg > 1:506944/1‑69 (MQ=255)aaCGTACCAGCGTTTCGCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCAGTTTGcgcc > 1:35778/1‑68 (MQ=255) tACCAGCGTTTCGCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCAGTTTGCGCCGCttt < 1:3397730/69‑1 (MQ=255) tACCAGCGTTTCGCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCAGTTTGCGCCGCttt < 1:2454764/69‑1 (MQ=255) tACCAGCGTTTCGCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCAGTTTGCGCCGCttt < 1:517216/69‑1 (MQ=255) tACCAGCGTTTCGCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCAGTTTGCGCCGCttt < 1:2282538/69‑1 (MQ=255) tACCAGCGTTTCGCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCAGTTTGCGCCGCttt < 1:2767818/69‑1 (MQ=255) tACCAGCGTTTCGCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCAGTTTGCGCCGCttt < 1:3050163/69‑1 (MQ=255) tACCAGCGTTTCGCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCAGTTTGCGCCGCttt < 1:2926524/69‑1 (MQ=255) cGTTTCGCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCGGTTTGCGCGGCttt > 1:1710850/1‑63 (MQ=255) cGTTTCGCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCGGTTTGCGCGGCttt > 1:963472/1‑63 (MQ=255) cGTTTCGCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCGGTTTGCGCGGCttt > 1:2249621/1‑63 (MQ=255) cGTTTCGCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCGGTTTGCGCGGCttt > 1:2160890/1‑63 (MQ=255) cGTTTCGCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCGGTTTGCGCGGCttt > 1:286603/1‑63 (MQ=255) cGTTTCGCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCGGTTTGCGCGGCttt > 1:2943886/1‑63 (MQ=255) cGTTTCGCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCGGTTTGCGCGGCttt > 1:3032615/1‑63 (MQ=255) cGTTTCGCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCGGTTTGCGCGGCttt > 1:1188463/1‑63 (MQ=255) cGTTTCGCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCGGTTTGCGCGGCttt > 1:1165873/1‑63 (MQ=255) cGTTTCGCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCGGTTTGCGCGGCttt > 1:3363881/1‑63 (MQ=255) cGTTTCGCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCGGTTTGCGCGGCttt > 1:14690/1‑63 (MQ=255) cGTTTCGCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCGGTTTGCGCGGCttt > 1:3372810/1‑63 (MQ=255) cGTTTCGCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCGGTTTGCGCGGCttt > 1:486503/1‑63 (MQ=255) cGTTTCGCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCGGTTTGCGCGGCtt > 1:2429996/1‑62 (MQ=255) tttCGCAGGTGGAATTAGTGTGTAGGGTAGACAACGCCAGGTGACCAGTTTGCGCCGCTTTAATAGCGa > 1:2747287/1‑69 (MQ=255) tttCGCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACTAGTTTGCGCCGCTTTAATAGCGa > 1:2560765/1‑69 (MQ=255) tttCGCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCAGTTTGCGCCGCTTTAATAGCGa > 1:1487417/1‑69 (MQ=255) tttCGCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCAGTTTGCGCCGCTTTAATAGCGa > 1:1317629/1‑69 (MQ=255) tttCGCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCAGTTTGCGCCGCTTTAATAGCGa > 1:2724292/1‑69 (MQ=255) tttCGCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCAGTTTGCGCCGCTTTAATAGCGa > 1:1177518/1‑69 (MQ=255) tttCGCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCAGTTTGCGCCGCTTTAATAGCGa > 1:1179684/1‑69 (MQ=255) ttCGCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCAGTTTGCGCCGCTTTAATAGCGAt < 1:2943869/69‑1 (MQ=255) ttCGCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCAGTTTGCGCCGCTTTAATAGCGAt < 1:1744569/69‑1 (MQ=255) ttCGCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCAGTTTGCGCCGCTTTAATAGCGAt < 1:301933/69‑1 (MQ=255) ttCGCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCAGTTTGCGCCGCTTTAATAGCGAt < 1:397864/69‑1 (MQ=255) ttCGCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCAGTTTGCGCCGCTTTAATAGCGAt < 1:1878140/69‑1 (MQ=255) ttCGCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCAGTTTGCGCCGCTTTAATAGCGAt < 1:261515/69‑1 (MQ=255) gCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCGGTTTGCGCGGCTTTAATGGCAAttt < 1:1641945/68‑3 (MQ=255) gCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCGGTTTGCGCGGCTTTAATGGCAAttt < 1:1194674/68‑3 (MQ=255) gCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCGGTTTGCGCGGCTTTAATGGCAAttt < 1:599672/68‑3 (MQ=255) gCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCGGTTTGCGCGGCTTTAATGGCAAttt < 1:1262631/68‑3 (MQ=255) gCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCGGTTTGCGCGGCTTTAATGGCAAttt < 1:1306135/68‑3 (MQ=255) gCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCGGTTTGCGCGGCTTTAATGGCAAttt < 1:883034/68‑3 (MQ=255) gCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCGGTTTGCGCGGCTTTAATGGCAAttt < 1:1158697/68‑3 (MQ=255) gCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCGGTTTGCGCGGCTTTAATGGCAAttt < 1:1334957/68‑3 (MQ=255) gCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCGGTTTGCGCGGCTTTAATGGCAAttt < 1:15095/68‑3 (MQ=255) gCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCGGTTTGCGCGGCTTTAATGGCAAttt < 1:164918/68‑3 (MQ=255) gCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCGGTTTGCGCGGCTTTAATGGCAAttt < 1:1805812/68‑3 (MQ=255) gCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCGGTTTGCGCGGCTTTAATGGCAAttt < 1:2190730/68‑3 (MQ=255) cAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCGGTTTGCTCGGCTTTAATGGCAAttt < 1:626443/67‑3 (MQ=255) cAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCGGTTTGCGCGGCTTTAATGGCAAttt < 1:2987855/67‑3 (MQ=255) aGGGTAGACAACACCAGGTGACCGGTTTGCGCGTCTTTAATGGCAAttt < 1:75487/49‑3 (MQ=21) acacCAGGTGACCAGTTTGCGCCGCTTTAATAGCGATCTCTGCTGTTTCGCCATCGCGGATCTCTCCg < 1:2491074/68‑1 (MQ=255) cAGGTGACCAGTTTGCGCCGCTTTAATAGCGATCTCTGCTGTTTCGCCATCGCGGATCTCTCCGATCat < 1:2011048/69‑1 (MQ=255) cAGGTGACCAGTTTGCGCCGCTTTAATAGCGATCTCTGCTGTTTCGCCATCGCGGATCTCTCCGATCat < 1:2240691/69‑1 (MQ=255) cgccgcTTTAATAGCGATCTCTGCTGTTTCGCCATCGCGGATCTCTCCGATCATGATGACGTCAGGAt < 1:1123555/68‑1 (MQ=255) cgccgcTTTAATAGCGATCTCTGCTGTTTCGCCATCGCGGATCTCTCCGATCATGATGACGTCAGGAt < 1:2575510/68‑1 (MQ=255) | AACGTACCAGCGTTTCGCAGGTGGAATTAGTGTGTAGGGTAGACAACACCAGGTGACCAGTTTGCGCCGCTTTAATAGCGATCTCTGCTGTTTCGCCATCGCGGATCTCTCCGATCATGATGACGTCAGGAT > minE/100858‑100989 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |