| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,238,281 | C→A | 31.9% | A80A (GCG→GCT) | edd ← | 6‑phosphogluconate dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,238,281 | 0 | C | A | 31.9% | 150.3 / 80.4 | 122 | A80A (GCG→GCT) | edd | 6‑phosphogluconate dehydratase |
| Reads supporting (aligned to +/- strand): ref base C (40/43); new base A (5/34); total (45/77) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.21e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
Only 100 of 126 total aligned reads displayed.
CAACCGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGTGATGATGGCGATATTGTTACGCAACATGCTTTTCAAA > minE/1238217‑1238344 | cAACCGTATTCGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGcgcg < 1:576685/68‑1 (MQ=255)cAACCGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGcgcg < 1:573811/68‑1 (MQ=255)cAACCGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGcgcg < 1:2537151/68‑1 (MQ=255) ccGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgag > 1:1972625/1‑68 (MQ=255) ccGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgag > 1:198522/1‑68 (MQ=255) ccGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgag > 1:757714/1‑68 (MQ=255) ccGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgag > 1:2017993/1‑68 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGagag < 1:2495447/69‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGagag < 1:13539/69‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGagag < 1:1738456/69‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGagag < 1:2881044/69‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGagag < 1:1590675/69‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:270508/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:2850736/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:3339844/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:3422119/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:2221869/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:2324371/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:2344713/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:780225/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:2852712/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:2911618/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:1484821/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:144446/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:865120/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:1332148/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:878674/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:1136545/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:918180/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:2960656/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:2992245/68‑1 (MQ=255) cGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATACTGTTCATAAGGCTGGTGAGCGgaga < 1:3276460/68‑1 (MQ=255) gCATTCGCTTCATGCAGGGCTTTACGAATGATTTCCGGATAGTGTTCATAAGGCTGGTGAGCGgaga < 1:905957/67‑1 (MQ=255) cGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTc > 1:3142839/1‑69 (MQ=255) ttCATGCAGGGCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGt < 1:3022383/68‑1 (MQ=255) ttCATGCAGGGCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGt < 1:2908377/68‑1 (MQ=255) ttCATGCAGGGCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGt < 1:3206794/68‑1 (MQ=255) ttCATGCAGGGCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGt < 1:387885/68‑1 (MQ=255) ttCATGCAGGGCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGt < 1:846842/68‑1 (MQ=255) ttCATGCAGGGCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGt < 1:1837220/68‑1 (MQ=255) tCATGCAGGGCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGTta < 1:3014665/69‑1 (MQ=255) tCATGCAGGGCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGTta < 1:777593/69‑1 (MQ=255) gCAGGGCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATagg < 1:481628/69‑1 (MQ=255) gggCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATaggag < 1:670588/68‑1 (MQ=255) ggCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGt < 1:202350/69‑1 (MQ=255) ggCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGt < 1:2673192/69‑1 (MQ=255) ggCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGt < 1:3235105/69‑1 (MQ=255) ggCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGt < 1:2629456/69‑1 (MQ=255) ggCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGt < 1:3196150/69‑1 (MQ=255) gCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGtg > 1:2164237/1‑69 (MQ=255) tttACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGtgat < 1:1944139/69‑1 (MQ=255) tttACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGtgat < 1:437516/69‑1 (MQ=255) tttACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGtgat < 1:965337/69‑1 (MQ=255) tttACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGtgat < 1:1105420/69‑1 (MQ=255) tttACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGtgat < 1:2556546/69‑1 (MQ=255) tttACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGtgat < 1:321781/69‑1 (MQ=255) ttACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGtgat < 1:1581279/68‑1 (MQ=255) tGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGc > 1:2077037/1‑41 (MQ=255) tGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGt > 1:860953/1‑45 (MQ=255) tGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGt > 1:339279/1‑45 (MQ=255) tGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGt > 1:638834/1‑45 (MQ=255) tGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGt > 1:1086388/1‑45 (MQ=255) tGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGt > 1:2766285/1‑45 (MQ=255) tGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGt > 1:3030286/1‑45 (MQ=255) tGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGt > 1:1111754/1‑45 (MQ=255) tGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGt > 1:2444151/1‑45 (MQ=255) tGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGt > 1:2584415/1‑45 (MQ=255) tGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGt > 1:2496521/1‑45 (MQ=255) gtgtTCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGTGATGATGGCGATATTGTTACGCaa < 1:818304/69‑1 (MQ=255) gtTCATAAGGCTTGTGCGCGGAGAGCATGTCGTTATAGGAGGTGATGATGGCGatat < 1:542900/57‑1 (MQ=255) ttCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGTGATGATGGCGatat < 1:812577/56‑1 (MQ=255) ttCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGTGATGATGGCGATATTGTTACGCAACAt > 1:433916/1‑69 (MQ=255) ttCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGTGATGATGGCGATATTGTTACGCAACAt > 1:716532/1‑69 (MQ=255) ttCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGTGATGATGGCGATATTGTTACGCAACAt > 1:2277907/1‑69 (MQ=255) ttCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGTGATGATGGCGATATTGTTACGCAACAt > 1:1604253/1‑69 (MQ=255) ttCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGTGATGATGGCGATATTGTTACGCAACAt > 1:2502241/1‑69 (MQ=255) ttCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGTGATGATGGCGATATTGTTACGCAACAt > 1:888683/1‑69 (MQ=255) ttCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGTGATGATGGCGATATTGTTACGCAACAt > 1:2619761/1‑69 (MQ=255) ttCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGTGATGATGGCGATATTGTTACGCAACAt > 1:1121757/1‑69 (MQ=255) ttCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGTGATGATGGCGATATTGTTACGCAACAt > 1:1026222/1‑69 (MQ=255) ttCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGTGATGATGGCGATATTGTTACGCAACAt > 1:2919136/1‑69 (MQ=255) cATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGTGATGATGGCGATATTGTTACGCAACATga > 1:2252103/1‑68 (MQ=255) cATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGTGATGATGGCGATATTGTTACGCAACATGc > 1:970349/1‑69 (MQ=255) aGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGTgatgat > 1:2683842/1‑42 (MQ=255) aGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGTGATGATGGCGATATTGTTACGCAACATGCttt > 1:2254219/1‑68 (MQ=255) aGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGTGATGATGGCGATATTGTTACGCAACATGCttt > 1:1353802/1‑68 (MQ=255) aGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGTGATGATGGCGATATTGTTACGCAACATGCttt > 1:190477/1‑68 (MQ=255) aGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGTGATGATGGCGATATTGTTACGCAACATGCttt > 1:2082423/1‑68 (MQ=255) aGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGTGATGATGGCGATATTGTTACGCAACATGCttt > 1:2632781/1‑68 (MQ=255) aGGCTGGTGAGCGGAGAGCATGTCGTTATAGGAGGtgatg < 1:846771/40‑1 (MQ=255) aGGCTGGTGAGCGGAGAGCATGTCGTTATAGGAGGtgatg < 1:538256/40‑1 (MQ=255) aGGCTGGTGAGCGGAGAGCATGTCGTTATAGGAGGtgatg < 1:358565/40‑1 (MQ=255) aGGCTGGTGAGCGGAGAGCATGTCGTTATAGGAGGtgatg < 1:3274895/40‑1 (MQ=255) aGGCTGGTGAGCGGAGAGCATGTCGTTATAGGAGGtgatg < 1:2885588/40‑1 (MQ=255) aGGCTGGTGAGCGGAGAGCATGTCGTTATAGGAGGtgatg < 1:97112/40‑1 (MQ=255) tGGTGCGCGGAGAGCATGTCGTTATAGGAGGTGATGATGGCGATATTGTTACGCAACATGCTTTTCaaa < 1:1878965/69‑1 (MQ=255) tGGTGCGCGGAGAGCATGTCGTTATAGGAGGTGATGATGGCGATATTGTTACGCAACATGCTTTTCaaa < 1:1765993/69‑1 (MQ=255) tGGTGCGCGGAGAGCATGTCGTTATAGGAGGTGATGATGGCGATATTGTTACGCAACATGCTTTTCaaa < 1:2586079/69‑1 (MQ=255) tGGTGCGCGGAGAGCATGTCGTTATAGGAGGTGATGATGGCGATATTGTTACGCAACATGCTTTTCaaa < 1:2631154/69‑1 (MQ=255) ggTGCGCGGAGAGCATGTCGTTATAGGAGGTGATGATGGCGATATTGTTACGCAACATGCTTTTCaaa < 1:1262766/68‑1 (MQ=255) | CAACCGCATTCGCTTCATGCAGGGCTTTACGAATGATTTCTGGATAGTGTTCATAAGGCTGGTGCGCGGAGAGCATGTCGTTATAGGAGGTGATGATGGCGATATTGTTACGCAACATGCTTTTCAAA > minE/1238217‑1238344 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |