| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,238,425 | A→G | 43.5% | T32T (ACT→ACC) | edd ← | 6‑phosphogluconate dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,238,425 | 0 | A | G | 43.5% | 13.8 / 53.3 | 69 | T32T (ACT→ACC) | edd | 6‑phosphogluconate dehydratase |
| Reads supporting (aligned to +/- strand): ref base A (25/14); new base G (19/11); total (44/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCAGCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCG > minE/1238369‑1238474 | gcagCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGt < 1:1395415/69‑1 (MQ=255) gtgCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCgg > 1:1498241/1‑65 (MQ=255) gtgCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCgg > 1:3365327/1‑65 (MQ=255) aGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGGGAGATa < 1:3459/68‑1 (MQ=255) aGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATa < 1:871073/68‑1 (MQ=255) aGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATa < 1:2877330/68‑1 (MQ=255) tACCGGATGCCAACTGAGAACGATGAACGGTCGAGGTTTTCGCTTGTTCTATc > 1:2760698/1‑53 (MQ=255) tACCGCATGCCAACTGCGAACGATGAACGGTCGAGGTTTTCGCTTGTTCTATc > 1:1060189/1‑53 (MQ=255) tACCGCATGCCAACTGCGAACGATGAACGGTCGAGGTTTTCGCTTGTTCTATc > 1:2195764/1‑53 (MQ=255) tACCGCATGCCAACTGCGAACGATGAACGGTCGAGGTTTTCGCTTGTTCTATc > 1:1837184/1‑53 (MQ=255) tACCGCATGCCAACTGCGAACGATGAACGGTCGAGGTTTTCGCTTGTTCTATc > 1:2262676/1‑53 (MQ=255) tACCGCATGCCAACTGCGAACGATGAACGGTCGAGGTTTTCGCTTGTTCTATc > 1:1748492/1‑53 (MQ=255) tACCGCATGCCAACTGCGAACGATGAACGGTCGAGGTTTTCGCTTGTTCTATc > 1:1665673/1‑53 (MQ=255) tACCGCATGCCAACTGCGAACGATGAACGGTCGAGGTTTTCGCTTGTTCTATc > 1:3041987/1‑53 (MQ=255) tACCGCATGCCAACTGCGAACGATGAACGGTCGAGGTTTTCGCTTGTTCTATc > 1:3156561/1‑53 (MQ=255) tACCGCATGCCAACTGCGAACGATGAACGGTCGAGGTTTTCGCTTGTTCTATc > 1:3374211/1‑53 (MQ=255) tACCGCATGCCAACTGCGAACGATGAACGGTCGAGGTTTTCGCTTGTTCTATc > 1:1629716/1‑53 (MQ=255) tACCGCATGCCAACTGCGAACGATGAACGGTCGAGGTTTTCGCTTGTTCTATc > 1:3417863/1‑53 (MQ=255) tACCGCATGCCAACTGCGAACGATGAACGGTCGAGGTTTTCGCTTGTTCTATc > 1:342942/1‑53 (MQ=255) tACCGCATGCCAACTGCGAACGATGAACGGTCGAGGTTTTCGCTTGTTCTATc > 1:368389/1‑53 (MQ=255) tACCGCATGCCAACTGCGAACGATGAACGGTCGAGGTTTTCGCTTGTTCTATc > 1:1284040/1‑53 (MQ=255) tACCGCATGCCAACTGCGAACGATGAACGGTCGAGGTTTTCGCTTGTTCTATc > 1:1151847/1‑53 (MQ=255) tACCGCATGCCAACTGCGAACGATGAACGGTCGAGGTTTTCGCTTGTTCTATc > 1:701109/1‑53 (MQ=255) tACCGCATGCCAACTGCGAACGATGAACGGTCGAGGTTTTCGCTTGTTCTATc > 1:1131239/1‑53 (MQ=255) tACCGCATGCCAACTGCGAACGATGAACGGTCGAGGTTTTCGCTTGTTCTATc > 1:1008576/1‑53 (MQ=255) tACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCag > 1:373941/1‑69 (MQ=255) tACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCag > 1:956171/1‑69 (MQ=255) tACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCag > 1:2159755/1‑69 (MQ=255) tACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCag > 1:2064023/1‑69 (MQ=255) tACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCag > 1:1632467/1‑69 (MQ=255) aTGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAg < 1:812179/69‑1 (MQ=255) aTGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAg < 1:2213628/69‑1 (MQ=255) aTGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAg < 1:1818946/69‑1 (MQ=255) aTGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAg < 1:2382978/69‑1 (MQ=255) aTGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAg < 1:3341196/69‑1 (MQ=255) aTGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAg < 1:1661228/69‑1 (MQ=255) tGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAg < 1:3051970/68‑1 (MQ=255) ccAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGtct > 1:3257711/1‑69 (MQ=255) ccAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGtct > 1:1065135/1‑69 (MQ=255) ccAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGtct > 1:1145512/1‑69 (MQ=255) ccAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGtct > 1:48280/1‑69 (MQ=255) ccAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGtct > 1:467466/1‑69 (MQ=255) ccAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGtct > 1:409852/1‑69 (MQ=255) ccAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGtct > 1:3227927/1‑69 (MQ=255) ccAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGtct > 1:2977130/1‑69 (MQ=255) ccAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGtct > 1:1988991/1‑69 (MQ=255) ccAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGtct > 1:1873665/1‑69 (MQ=255) ccAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGtct > 1:1849443/1‑69 (MQ=255) ccAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGtct > 1:2810939/1‑69 (MQ=255) ccAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGtct > 1:1661476/1‑69 (MQ=255) ccAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGtct > 1:301222/1‑69 (MQ=255) ccAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGtc > 1:813580/1‑68 (MQ=255) tGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTc < 1:1574055/35‑1 (MQ=255) tGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTc < 1:1336601/35‑1 (MQ=255) tGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTc < 1:1654862/35‑1 (MQ=255) gCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTcgcgcg > 1:2055635/1‑69 (MQ=255) gCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTcgcgcg > 1:880344/1‑69 (MQ=255) gCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTcgcgcg > 1:3094379/1‑69 (MQ=255) cGGTCGAGGTTTTCGCTTGTTCTATCCGGGCGAGGTAAGCaga < 1:2548825/43‑1 (MQ=255) cGGTCGAGGTTTTCGCTTGTTCTATCCGGGCGAGGTAAGCaga < 1:1114819/43‑1 (MQ=255) cGGTCGAGGTTTTCGCTTGTTCTATCCGGGCGAGGTAAGCaga < 1:2209792/43‑1 (MQ=255) cGGTCGAGGTTTTCGCTTGTTCTATCCGGGCGAGGTAAGCaga < 1:2213931/43‑1 (MQ=255) cGGTCGAGGTTTTCGCTTGTTCTATCCGGGCGAGGTAAGCaga < 1:1653116/43‑1 (MQ=255) cGGTCGAGGTTTTCGCTTGTTCTATCCGGGCGAGGTAAGCaga < 1:1171209/43‑1 (MQ=255) cGGTCGAGGTTTTCGCTTGTTCTATCCGGGCGAGGTAAGCaga < 1:371877/43‑1 (MQ=255) cGGTCGAGGTTTTCGCTTGTTCTATCCGGGCGAGGTAAGCaga < 1:348606/43‑1 (MQ=255) cGGTCGAGGTTTTCGCTTGTTCTATCCGGGCGAGGTAAGCaga < 1:1425416/43‑1 (MQ=255) cGGTCGAGGTTTTCGCTTGTTCTATCCGGGCGAGGTAAGCaga < 1:1515228/43‑1 (MQ=255) cGGTCGAGGTTTTCGCTTGTTCTATCCGGGCGAGGTAAGCaga < 1:1747400/43‑1 (MQ=255) | GCAGCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCG > minE/1238369‑1238474 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |