| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,239,208 | C→T | 40.6% | L341L (CTG→CTA) | zwf ← | glucose‑6‑phosphate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,239,208 | 0 | C | T | 40.6% | 26.1 / 42.8 | 64 | L341L (CTG→CTA) | zwf | glucose‑6‑phosphate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (4/34); new base T (12/14); total (16/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.52e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
TTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCG > minE/1239145‑1239268 | ttCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACg < 1:1725034/69‑1 (MQ=255) tCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGt < 1:1168361/69‑1 (MQ=255) tCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGt < 1:2788838/69‑1 (MQ=255) tCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGt < 1:3407270/69‑1 (MQ=255) tCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGt < 1:2096884/69‑1 (MQ=255) cTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGt < 1:2426714/68‑1 (MQ=255) cTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGt < 1:201772/68‑1 (MQ=255) cAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGt < 1:876510/69‑1 (MQ=255) cAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGt < 1:2424741/69‑1 (MQ=255) cAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGt < 1:2330842/69‑1 (MQ=255) cAGATTCAGTTCAGGTGGTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGt < 1:2265074/69‑1 (MQ=255) tcagGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAg < 1:1027921/69‑1 (MQ=255) tcagGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAg < 1:2663200/69‑1 (MQ=255) tcagGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAg < 1:2667282/69‑1 (MQ=255) tcagGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAg < 1:2196972/69‑1 (MQ=255) tcagGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAg > 1:2196092/1‑69 (MQ=255) tcagGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAg < 1:851632/69‑1 (MQ=255) tcagGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAg < 1:1324071/69‑1 (MQ=255) tcagGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAg < 1:1716368/69‑1 (MQ=255) tcagGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAg < 1:1588770/69‑1 (MQ=255) cagGTGTTTTGAAATATACCACGACTTCAGAACATTTGGTCGGTAGACGTTTACCAg < 1:3213126/57‑1 (MQ=255) cagGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGTAGACGTTTACCAg < 1:1923860/57‑1 (MQ=255) cagGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGTAGACGTTTACCAg < 1:2501644/57‑1 (MQ=255) cagGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGTAGACGTTTACCAg < 1:121240/57‑1 (MQ=255) cagGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGTAGACGTTTACCAg < 1:3424868/57‑1 (MQ=255) cagGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGTAGACGTTTACCAg < 1:2208782/57‑1 (MQ=255) cagGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGTAGACGTTTACCAg < 1:1597026/57‑1 (MQ=255) cagGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGTAGACGTTTACCAg < 1:3074962/57‑1 (MQ=255) cagGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGTAGACGTTTACCAg < 1:314079/57‑1 (MQ=255) cagGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGTAGACGTTTACCAg < 1:2061033/57‑1 (MQ=255) cagGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGTAGACGTTTACCAg < 1:3161002/57‑1 (MQ=255) cagGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGTAGACGTTTACCAg < 1:2032274/57‑1 (MQ=255) agGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCAGTAGACGTTTACCAg < 1:3271782/56‑1 (MQ=255) ttGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCATGTAGAATgg < 1:2364134/65‑1 (MQ=255) ttGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATgg < 1:3403947/65‑1 (MQ=255) ttGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATgg < 1:291193/65‑1 (MQ=255) ttGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATgg < 1:2903351/65‑1 (MQ=255) ttGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATgg < 1:922956/65‑1 (MQ=255) ttGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATgg < 1:1115582/65‑1 (MQ=255) ttGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATgg < 1:2446488/65‑1 (MQ=255) ttGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATgg < 1:2320257/65‑1 (MQ=255) ttGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATgg < 1:2049145/65‑1 (MQ=255) ttGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGcaca > 1:943904/1‑69 (MQ=255) tGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATgg < 1:2613479/64‑1 (MQ=255) aaTAGACCACGACTTCAGAACATTTGGTCGGTAGACGTTTACCAg < 1:4197/45‑1 (MQ=255) tAGACCACGACTTCAGAACATTTGGTCGGCAGACGttt < 1:52686/38‑1 (MQ=255) tAGACCACGACTTCAGAACATTTGGTCGGCAGACGttt < 1:2156823/38‑1 (MQ=255) tAGACCACGACTTCAGAACATTTGGTCGGCAGACGttt < 1:508232/38‑1 (MQ=255) tAGACCACGACTTCAGAACATTTGGTAGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCgg > 1:1276224/1‑67 (MQ=255) tAGACCACGACTTCAGAACATTAGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCgg > 1:1616264/1‑67 (MQ=255) ttCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGtt < 1:955326/69‑1 (MQ=255) ttCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGtt < 1:212855/69‑1 (MQ=255) ttGGTCGGTAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGGGCCAGTTATCAATGTCg > 1:1921209/1‑69 (MQ=255) ttGGTCGGTAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTc > 1:365602/1‑68 (MQ=255) ttGGTCGGTAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCg > 1:1239862/1‑69 (MQ=255) ttGGTCGGTAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCg > 1:1151115/1‑69 (MQ=255) ttGGTCGGTAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCg > 1:1457844/1‑69 (MQ=255) ttGGTCGGTAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCg > 1:583292/1‑69 (MQ=255) ttGGTCGGTAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCg > 1:1455803/1‑69 (MQ=255) ttGGTCGGTAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCg > 1:1856511/1‑69 (MQ=255) ttGGTCGGTAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCg > 1:2941740/1‑69 (MQ=255) ttGGTCGGTAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCg > 1:2569572/1‑69 (MQ=255) ttGGTCGGTAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAAAGTc > 1:839689/1‑68 (MQ=255) ttGGTCGGTAGACGTTTACCAGTACGCAGGTAGAATGGCACACAGGCCCAGCGCCAGTTATCAATGTc > 1:21141/1‑68 (MQ=255) | TTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCG > minE/1239145‑1239268 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |