Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,246,967	A→G	100%	L130L (CTA→CTG)	znuC →	zinc transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,246,967	0	A	G	96.7%	61.5 / ‑5.1	28	L130L (CTA→CTG)	znuC	zinc transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (16/11);  total (16/12)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGT  >  minE/1246913‑1247005                                                       |                                       tcTGATTAACGCACCGATGCAAAAGCTCTCGGGTGGCGAAACGCAGCGTGTACTGTTAGCGCGAGCAtt                          <  1:647382/68‑1 (MQ=255) tcTGATTAACGCACCGATGCAAAAGCTCTCGGGTGGCGAAACGCAGCGTGTACTGTTAGCGCGAGCAtt                          <  1:1684465/68‑1 (MQ=255) tcTGATTAACGCACCGATGCAAAAGCTCTCGGGTGGCGAAACGCAGCGTGTACTGTTAGCGCGAGCAtt                          <  1:1946544/68‑1 (MQ=255) tcTGATTAACGCACCGATGCAAAAGCTCTCGGGTGGCGAAACGCAGCGTGTACTGTTAGCGCGAGCAtt                          <  1:199684/68‑1 (MQ=255) tcTGATTAACGCACCGATGCAAAAGCTCTCGGGTGGCGAAACGCAGCGTGTACTGTTAGCGCGAGCAtt                          <  1:400016/68‑1 (MQ=255) tcTGATTAACGCACCGATGCAAAAGCTCTCGGGTGGCGAAACGCAGCGTGTACTGTTAGCGCGAGCAtt                          <  1:2062162/68‑1 (MQ=255) tcTGATTAACGCACCGATGCAAAAGCTCTCGGGTGGCGAAACGCAGCGTGTACTGTTAGCGCGAGCAtt                          <  1:2066186/68‑1 (MQ=255) tcTGATTAACGCACCGATGCAAAAGCTCTCGGGTGGCGAAACGCAGCGTGTACTGTTAGCGCGAGCAtt                          <  1:738896/68‑1 (MQ=255) tcTGATTAACGCACCGATGCAAAAGCTCTCGGGTGGCGAAACGCAGCGTGTACTGTTAGCGCGAGCAtt                          <  1:3049573/68‑1 (MQ=255) tcTGATTAACGCACCGATGCAAAAGCTCTCGGGTGGCGAAACGCAGCGTGTACTGTTAGCGCGAGCAtt                          <  1:2940353/68‑1 (MQ=255)  cTGATTAACGCACCGATGCAAAAGCTCTCGGGTGGCGAAACGCAGCGTGTACTGTTAGCGCGAGCAtt                          <  1:676046/68‑1 (MQ=255)             aCCGATGCAAAAGCTCTCGGGTGGCGAAACGCAGCGTGTACTGTTAGCGCGAGCATTGTTAAATCgac               >  1:1277028/1‑66 (MQ=255)             aCCGATGCAAAAGCTCTCGGGTGGCGAAACGCAGCGTGTACTGTTAGCGCGAGCATTGTTAAATCgac               >  1:3243932/1‑66 (MQ=255)             aCCGATGCAAAAGCTCTCGGGTGGCGAAACGCAGCGTGTACTGTTAGCGCGAGCATTGTTAAATCgac               >  1:3055903/1‑66 (MQ=255)             aCCGATGCAAAAGCTCTCGGGTGGCGAAACGCAGCGTGTACTGTTAGCGCGAGCATTGTTAAATCgac               >  1:2502318/1‑66 (MQ=255)             aCCGATGCAAAAGCTCTCGGGTGGCGAAACGCAGCGTGTACTGTTAGCGCGAGCATTGTTAAATCgac               >  1:894/1‑66 (MQ=255)             aCCGATGCAAAAGCTCTCGGGTGGCGAAACGCAGCGTGTACTGTTAGCGCGAGCATTGTTAAATCgac               >  1:2300454/1‑66 (MQ=255)             aCCGATGCAAAAGCTCTCGGGTGGCGAAACGCAGCGTGTACTGTTAGCGCGAGCATTGTTAAATCgac               >  1:2181258/1‑66 (MQ=255)             aCCGATGCAAAAGCTCTCGGGTGGCGAAACGCAGCGTGTACTGTTAGCGCGAGCATTGTTAAATCgac               >  1:1571005/1‑66 (MQ=255)                   gCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCa           <  1:2303009/66‑1 (MQ=255)                         gCTCTCGGGTGGCGAAACGCAGCGTGTACTGTTAgcg                                  >  1:2008672/1‑37 (MQ=37)                         gCTCTCGGGTGGCGAAACGCAGCGTGTACTGTTAGCGCGAGCATTGTTAAATCGACCGCAATTATTAGt  >  1:676705/1‑69 (MQ=255)                         gCTCTCGGGTGGCGAAACGCAGCGTGTACTGTTAGCGCGAGCATTGTTAAATCGACCGCAATTATTAGt  >  1:995268/1‑69 (MQ=255)                         gCTCTCGGGTGGCGAAACGCAGCGTGTACTGTTAGCGCGAGCATTGTTAAATCGACCGCAATTATTAGt  >  1:316605/1‑69 (MQ=255)                         gCTCTCGGGTGGCGAAACGCAGCGTGTACTGTTAGCGCGAGCATTGTTAAATCGACCGCAATTATTAGt  >  1:3075790/1‑69 (MQ=255)                         gCTCTCGGGTGGCGAAACGCAGCGTGTACTGTTAGCGCGAGCATTGTTAAATCGACCGCAATTATTAGt  >  1:2439770/1‑69 (MQ=255)                         gCTCTCGGGTGGCGAAACGCAGCGTGTACTGTTAGCGCGAGCATTGTTAAATCGACCGCAATTATTAGt  >  1:1428572/1‑69 (MQ=255)                         gCTCTCGGGAGGCGAAACGCAGCGTGTACTGTTAGCGCGAGCATTGTTAAATCGACCGCAATTATTAGt  >  1:700543/1‑69 (MQ=255)                                                       |                                       CCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGT  >  minE/1246913‑1247005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑