Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,247,830 A→C 22.3% R167R (CGA→CGC)  znuB → zinc transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,247,8300AC22.3% 157.8 / 46.7 103R167R (CGA→CGC) znuBzinc transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (36/44);  new base C (7/16);  total (43/60)
Fisher's exact test for biased strand distribution p-value = 2.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

GGCGCAATTTGCTGTCAATGACGATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGATTGGTGTAGCGATGAAATTTGTCGGTGCG  >  minE/1247763‑1247896
                                                                   |                                                                  
ggCGCAATTTGCTGTCAATGACGATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGa                                                                    >  1:1829143/1‑68 (MQ=255)
ggCGCAATTTGCTGTCAATGACGATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGa                                                                    >  1:952470/1‑68 (MQ=255)
ggCGCAATTTGCTGTCAATGACGATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGa                                                                    >  1:1151133/1‑68 (MQ=255)
ggCGCAATTTGCTGTCAATGACGATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGa                                                                    >  1:3283/1‑68 (MQ=255)
ggCGCAATTTGCTGTCAATGACGATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGa                                                                    >  1:933661/1‑68 (MQ=255)
ggCGCAATTTGCTGTCAATGACGATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGa                                                                    >  1:1525902/1‑68 (MQ=255)
ggCGCAATTTGCTGTCAATGACGATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGa                                                                    >  1:325236/1‑68 (MQ=255)
ggCGCAATTTGCTGTCAATGACGATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGa                                                                    >  1:2307440/1‑68 (MQ=255)
 gcgcAATTTGCTGTCAATGACGATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAg                                                                   >  1:424681/1‑68 (MQ=255)
 gcgcAATTTGCTGTCAATGACGATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAg                                                                   >  1:225498/1‑68 (MQ=255)
 gcgcAATTTGCTGTCAATGACGATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAg                                                                   >  1:2891350/1‑68 (MQ=255)
 gcgcAATTTGCTGTCAATGACGATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAg                                                                   >  1:3074747/1‑68 (MQ=255)
 gcgcAATTTGCTGTCAATGACGATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAg                                                                   >  1:1425311/1‑68 (MQ=255)
             gTCAATGACGATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAGTGAAAttgttgtt                                                      <  1:2594175/69‑1 (MQ=255)
             gTCAATGACGATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAGTGAAAttgttgtt                                                      <  1:799647/69‑1 (MQ=255)
             gTCAATGACGATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAGTGAAAttgttgtt                                                      <  1:2590211/69‑1 (MQ=255)
              tCAATGACGATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAGTGAAAttgttgttg                                                     >  1:3114942/1‑69 (MQ=255)
              tCAATGACGATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAGTGAAAttgttgttg                                                     >  1:937982/1‑69 (MQ=255)
              tCAATGACGATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAGTGAAAttgttgttg                                                     >  1:700110/1‑69 (MQ=255)
              tCAATGACGATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAGTGAAAttgttgttg                                                     >  1:395355/1‑69 (MQ=255)
                aaTGATGATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAGTGAAATTGTTGTTGAt                                                   >  1:2439266/1‑69 (MQ=255)
                aaTGACGATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAGTg                                                                 >  1:102112/1‑55 (MQ=255)
                aaTGACGATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAGTGAAATTGTTGTTGAt                                                   >  1:3258416/1‑69 (MQ=255)
                aaTGACGATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAGTGAAATTGTTGTTGAt                                                   >  1:2196107/1‑69 (MQ=255)
                aaTGACGATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAGTGAAATTGTTGTTGAt                                                   <  1:3393992/69‑1 (MQ=255)
                aaTGACGATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAGTGAAATTGTTGTTGAt                                                   >  1:2826730/1‑69 (MQ=255)
                aaTGACGATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAGTGAAATTGTTGTTGAt                                                   <  1:407818/69‑1 (MQ=255)
                aaTGACGATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAGTGAAATTGTTGTTGAt                                                   >  1:478728/1‑69 (MQ=255)
                aaTGACGATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAGTGAAATTGTTGTTGAt                                                   >  1:488899/1‑69 (MQ=255)
                aaTGACGATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAGTGAAATTGTTGTTGAt                                                   >  1:75060/1‑69 (MQ=255)
                aaTGACGATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAGTGAAATTGTTGTTGAt                                                   <  1:1550776/69‑1 (MQ=255)
                aaTGACGATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAGTGAAATTGTTGTTGAt                                                   >  1:782025/1‑69 (MQ=255)
                aaTGACGATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAGTGAAATTGTTGTTGAt                                                   >  1:1174850/1‑69 (MQ=255)
                aaTGACGATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAGTGAAATTGTTGTTGAt                                                   >  1:1203913/1‑69 (MQ=255)
                      gATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAGTGAAATTGTTGTTGATGCTGGt                                             <  1:2316977/69‑1 (MQ=255)
                      gATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAGTGAAATTGTTGTTGATGCTGGt                                             <  1:1028900/69‑1 (MQ=255)
                      gATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAGTGAAATTGTTGTTGATGCTGGt                                             <  1:935532/69‑1 (MQ=255)
                      gATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAGTGAAATTGTTGTTGATGCTGGt                                             <  1:908325/69‑1 (MQ=255)
                      gATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAGTGAAATTGTTGTTGATGCTGGt                                             <  1:878194/69‑1 (MQ=255)
                      gATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAGTGAAATTGTTGTTGATGCTGGt                                             <  1:3254287/69‑1 (MQ=255)
                      gATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAGTGAAATTGTTGTTGATGCTGGt                                             <  1:2265989/69‑1 (MQ=255)
                      gATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAGTGAAATTGTTGTTGATGCTGGt                                             <  1:1596212/69‑1 (MQ=255)
                          aGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAGTGAAATTGTTGTTGATGCTGGTGAc                                          >  1:859343/1‑68 (MQ=255)
                          aGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAGTGAAATTGTTGTTGATGCTGGTGAc                                          >  1:2863322/1‑68 (MQ=255)
                            cccGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAGTGAAATTGTTGTTGATGCTGGTGACgg                                        <  1:419854/68‑1 (MQ=255)
                            cccGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAGTGAAATTGTTGTTGATGCTGGTGACgg                                        <  1:909688/68‑1 (MQ=255)
                            cccGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAGTGAAATTGTTGTTGATGCTGGTGACgg                                        <  1:964644/68‑1 (MQ=255)
                            cccGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAGTGAAATTGTTGTTGATGCTGGTGACgg                                        <  1:2123268/68‑1 (MQ=255)
                            cccGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAGTGAAATTGTTGTTGATGCTGGTGACgg                                        <  1:2653911/68‑1 (MQ=255)
                                      gCGTTTGTTGATGGTGTGAAATTACAGCGCGTGAAATTGTTGTTGAt                                                   >  1:1598963/1‑47 (MQ=255)
                                      gCGTTTGTTGATGGTGTGAAATTACAGCGCGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGAtt                             >  1:1247197/1‑69 (MQ=255)
                                      gCGTTTGTTGATGGTGTGAAATTACAGCGCGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGAtt                             >  1:1264717/1‑69 (MQ=255)
                                      gCGTTTGTTGATGGTGTGAAATTACAGCGCGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGAtt                             >  1:3142686/1‑69 (MQ=255)
                                      gCGTTTGTTGATGGTGTGAAATTACAGCGCGTGAAATTGTTGTTGATGCTGGTGACGCCATTGACGAtt                             >  1:3211638/1‑69 (MQ=255)
                                      gCGTTTGTTGATGGTGTCAAATTACAGCGCGTCAAATTGTTGTTGATGc                                                 >  1:3247038/1‑49 (MQ=38)
                                           tgttgATGGTGTGAAATTACAGCGAGTGAAATTGTTGTTGATGCt                                                >  1:3078677/1‑45 (MQ=255)
                                           tgttgATGGTGTGAAATTACAGCGAGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGATTGgtgt                        >  1:1265010/1‑69 (MQ=255)
                                                   gtgtGAAATTACAGCGAGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGATTGGTGTAGCGATGa                <  1:683783/69‑1 (MQ=255)
                                                   gtgtGAAATTACAGCGAGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGATTGGTGTAGCGATGa                <  1:2044368/69‑1 (MQ=255)
                                                   gtgtGAAATTACAGCGAGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGATTGGTGTAGCGATGa                <  1:592627/69‑1 (MQ=255)
                                                   gtgtGAAATTACAGCGAGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGATTGGTGTAGCGATGa                <  1:516052/69‑1 (MQ=255)
                                                   gtgtGAAATTACAGCGAGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGATTGGTGTAGCGATGa                <  1:1879076/69‑1 (MQ=255)
                                                   gtgtGAAATTACAGCGAGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGATTGGTGTAGCGATGa                <  1:1997492/69‑1 (MQ=255)
                                                   gtgtGAAATTACAGCGAGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGATTGGTGTAGCGATGa                <  1:72312/69‑1 (MQ=255)
                                                   gtgtGAAATTACAGCGAGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGATTGGTGTAGCGATGa                <  1:1559168/69‑1 (MQ=255)
                                                   gtgtGAAATTACAGCGAGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGATTGGTGTAGCGATGa                <  1:1304463/69‑1 (MQ=255)
                                                   gtgtGAAATTACAGCGAGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGATTGGTGTAGCGATGa                <  1:1072709/69‑1 (MQ=255)
                                                   gtgtGAAATTACAGCGAGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGATTGGTGTAGCGATGa                <  1:2730179/69‑1 (MQ=255)
                                                   gtgtGAAATTACAGCGAGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGATTGGTGTAGCGATGa                <  1:2322738/69‑1 (MQ=255)
                                                   gtgtGAAATTACAGCGAGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGATTGGTGTAGCGATGa                <  1:276954/69‑1 (MQ=255)
                                                   gtgtGAAATTACAGCGAGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGATTGGTGTAGCGATGa                <  1:2627905/69‑1 (MQ=255)
                                                   gtgtGAAATTACAGCGAGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGATTGGTGTAGCGATGa                <  1:2614262/69‑1 (MQ=255)
                                                   gtgtGAAATTACAGCGAGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGATTGGTGTAGCGATGa                <  1:2871947/69‑1 (MQ=255)
                                                   gtgtGAAATTACAGCGAGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGATTGGTGTAGCGATGa                <  1:311817/69‑1 (MQ=255)
                                                   gtgtGAAATTACAGCGAGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGATTGGTGTAGCGATGa                <  1:2444506/69‑1 (MQ=255)
                                                   gtgtGAAATTACAGCGAGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGATTGGTGTAGCGATGa                <  1:2293383/69‑1 (MQ=255)
                                                   gtgtGAAATTACAGCGAGTGAAATTGTTCTTGATGCTGGTGACGGCATTGACGATTGGTGTAGCGATGa                <  1:1879189/69‑1 (MQ=255)
                                                     gtgAAATTTCAGCGAGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGATTGGTGTAGCGATGaaa              >  1:604858/1‑69 (MQ=255)
                                                     gtgAAATTACAGCGAGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGATTGGTGTAGCGATGaaa              >  1:1875708/1‑69 (MQ=255)
                                                      tgAAATTACAGCGCGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGATTGgtg                         <  1:483328/57‑1 (MQ=255)
                                                      tgAAATTACAGCGCGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGATTGgtg                         <  1:1770484/57‑1 (MQ=255)
                                                      tgAAATTACAGCGCGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGATTGgtg                         <  1:3083802/57‑1 (MQ=255)
                                                      tgAAATTACAGCGCGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGATTGgtg                         <  1:1172328/57‑1 (MQ=255)
                                                      tgAAATTACAGCGCGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGATTGgtg                         <  1:2344504/57‑1 (MQ=255)
                                                      tgAAATTACAGCGCGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGATTGgtg                         <  1:2984471/57‑1 (MQ=255)
                                                      tgAAATTACAGCGCGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGATTGgtg                         <  1:702455/57‑1 (MQ=255)
                                                      tgAAATTACAGCGCGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGATTGgtg                         <  1:2473653/57‑1 (MQ=255)
                                                      tgAAATTACAGCGCGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGATTGgtg                         <  1:3139304/57‑1 (MQ=255)
                                                      tgAAATTACAGCGCGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGATTGgtg                         <  1:3293368/57‑1 (MQ=255)
                                                      tgAAATTACAGCGCGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGATTGgtg                         <  1:1254617/57‑1 (MQ=255)
                                                      tgAAATTACAGCGCGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGATTGgtg                         <  1:2814022/57‑1 (MQ=255)
                                                      tgAAATTACAGCGCGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGATTGgtg                         <  1:3358382/57‑1 (MQ=255)
                                                      tgAAATTACAGCGAGTGAAATTGTTGTTGATGCTGGt                                             <  1:2265854/37‑1 (MQ=255)
                                                       gAAATTACAGCGCGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGATTGgtg                         <  1:187866/56‑1 (MQ=255)
                                                        aaaTTACAGCGAGTGAAATTGTTGTTGATGCTGGTGACGGCAtt                                    >  1:3321253/1‑44 (MQ=255)
                                                        aaaTTACAGCGAGTGAAATTGTTGTTGATGCTGGTGACGGCAtt                                    >  1:1364166/1‑44 (MQ=255)
                                                        aaaTTACAGCGAGTGAAATTGTTGTTGATGCTGGTGACGGCAt                                     >  1:1140039/1‑43 (MQ=255)
                                                            tACAGCGCGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGAttgttg                         <  1:3109229/51‑1 (MQ=255)
                                                             aCAGCGAGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGATTGGTGTAGCGATGAAATTTGTCg       >  1:1400238/1‑68 (MQ=255)
                                                                 cGAGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGATTGGTGTAGCGATGAAATTTGTCGGTGCg  <  1:1364788/69‑1 (MQ=255)
                                                                   |                                                                  
GGCGCAATTTGCTGTCAATGACGATAAGCCCGGATCTGGCGTTTGTTGATGGTGTGAAATTACAGCGAGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGATTGGTGTAGCGATGAAATTTGTCGGTGCG  >  minE/1247763‑1247896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑