| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,251,089 | T→C | 24.4% | Q68Q (CAA→CAG) | ruvC ← | component of RuvABC resolvasome, endonuclease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,251,089 | 0 | T | C | 24.4% | 129.5 / 37.9 | 106 | Q68Q (CAA→CAG) | ruvC | component of RuvABC resolvasome, endonuclease |
| Reads supporting (aligned to +/- strand): ref base T (50/30); new base C (11/15); total (61/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.09e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 108 total aligned reads displayed.
ACAATCGCCACGCCGCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCACGCCCGCATAGATG > minE/1251024‑1251152 | acaATCGCCACGCCGCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACtt > 1:2145472/1‑67 (MQ=255)acaATCGCCACGCCGCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACtt > 1:784654/1‑67 (MQ=255)acaATCGCCACGCCGCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTg > 1:2972533/1‑68 (MQ=255)acaATCGCCACGCCGCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTg > 1:3053494/1‑68 (MQ=255)acaATCGCCACGCCGCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTg > 1:373592/1‑68 (MQ=255)acaATCGCCACGCCGCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTg > 1:1974571/1‑68 (MQ=255)acaATCGCCACGCCGCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTg > 1:808838/1‑68 (MQ=255)acaATCGCCACGCCGCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTg > 1:1953025/1‑68 (MQ=255)acaATAGCCACGCCGCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTg > 1:3377251/1‑68 (MQ=255) aaTCGCCACGCCGCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTc < 1:2499261/69‑1 (MQ=255) aaTCGCCACGCCGCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTc < 1:331657/69‑1 (MQ=255) aaTCGCCACGCCGCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTc < 1:1883307/69‑1 (MQ=255) aTCGCCACGCCGCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTc > 1:1827556/1‑68 (MQ=255) aTCGCCACGCCGCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCa > 1:1082699/1‑69 (MQ=255) aTCGCCACGCCGCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCa > 1:798615/1‑69 (MQ=255) aTCGCCACGCCGCGCGCCTGCCCCAGTTTCAGGGCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTc > 1:386459/1‑68 (MQ=255) aTCGCCACGCCGCGCGCCTGCCCCAGTTTCAGGGCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTc > 1:177884/1‑68 (MQ=255) aTCGCCACGCCGCGCGCCTGCCCCAGTTTCAGGGCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTCa > 1:81966/1‑69 (MQ=255) aTCGCCACGCCGCGCGCCTGCCCCAGTTTCAGGGCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTCa > 1:3260444/1‑69 (MQ=255) aTCGCCACGCCGCGCGCCTGCCCCAGTTTCAGGGCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTCa > 1:1239713/1‑69 (MQ=255) aTCGCCACGCCGCGCGCCTGCCCCAGTTTCAGGGCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTCa > 1:2098883/1‑69 (MQ=255) aTCGCCACGCCGCGCGCCTGCCCCAGTTTCAGGGCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTCa > 1:2086793/1‑69 (MQ=255) aTCGCCACGCCGCGCGCCTGCCCCAGTTTCAGGGCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTCa > 1:145862/1‑69 (MQ=255) aTCGCCACGCCGCGCGCCTGCCCCAGTTTCAGGGCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTCa > 1:2949666/1‑69 (MQ=255) aTCGCCACGCCGCGCGCCTGCCCCAGTTTCAGGGCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTCa > 1:658588/1‑69 (MQ=255) cACGCCGCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATgg > 1:470549/1‑68 (MQ=255) cACGCCGCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATgg > 1:2667372/1‑68 (MQ=255) cACGCCGCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATgg > 1:2977027/1‑68 (MQ=255) cACGCCGCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATg > 1:474589/1‑67 (MQ=255) ccgcGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGAGTTGTTCAATGGTGaa > 1:1296165/1‑68 (MQ=255) ccgcGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGaa > 1:2191210/1‑68 (MQ=255) ccgcGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGaa > 1:887554/1‑68 (MQ=255) ccgcGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGaa > 1:885634/1‑68 (MQ=255) ccgcGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGaa > 1:1201137/1‑68 (MQ=255) gcgcCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAaata > 1:2168732/1‑67 (MQ=255) gcgcCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAaata > 1:817470/1‑67 (MQ=255) gcgcCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAaata > 1:1784721/1‑67 (MQ=255) gcgcCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAaata > 1:2346462/1‑67 (MQ=255) gcgcCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAaata > 1:2537255/1‑67 (MQ=255) gccccAGTTTCAGGGCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTCaa < 1:497222/49‑1 (MQ=255) cccAGTTTCAGGGCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTCaa < 1:16888/49‑1 (MQ=255) ccAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTgg < 1:410884/68‑1 (MQ=255) ccAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTgg < 1:3270871/68‑1 (MQ=255) ccAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTgg < 1:1801997/68‑1 (MQ=255) ttCAGGGCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTCaa < 1:220693/43‑1 (MQ=38) ttCAGGGCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTCAATg < 1:3253014/45‑1 (MQ=255) tCAGGGCTGAGTCAGCGTTCTTCGCCCTAAAGACCTGTTCAATg < 1:3072755/44‑1 (MQ=38) tCAGGGCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTCAATg < 1:2211311/44‑1 (MQ=38) tCAGGGCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTCAATg < 1:2962993/44‑1 (MQ=38) tCAGGGCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTCAATg < 1:3005390/44‑1 (MQ=38) tCAGGGCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTCAATg < 1:2358009/44‑1 (MQ=38) tCAGGGCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTCAATg < 1:2333987/44‑1 (MQ=38) tCAGGGCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTCAATg < 1:2240489/44‑1 (MQ=38) tCAGGGCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTCAATg < 1:1500478/44‑1 (MQ=38) ggCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTCAATg < 1:2677905/40‑1 (MQ=38) gCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTCaa < 1:2789511/37‑1 (MQ=38) gAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGAtt < 1:2426511/69‑1 (MQ=255) gAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGAtt < 1:1681497/69‑1 (MQ=255) gAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGAtt < 1:758639/69‑1 (MQ=255) aGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat < 1:3271125/60‑1 (MQ=255) aGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat < 1:3149686/60‑1 (MQ=255) aGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat < 1:778089/60‑1 (MQ=255) aGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat < 1:611052/60‑1 (MQ=255) aGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat < 1:1190157/60‑1 (MQ=255) aGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat < 1:2918797/60‑1 (MQ=255) gTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCAc < 1:1371446/69‑1 (MQ=255) gTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCAc < 1:1374081/69‑1 (MQ=255) gTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCAc < 1:1734260/69‑1 (MQ=255) gTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCAc < 1:1518814/69‑1 (MQ=255) gTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCAc < 1:2211864/69‑1 (MQ=255) gTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCAc < 1:2071545/69‑1 (MQ=255) aaGACTTGTTCAATGGCTAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCACGCCCGCATAGATg > 1:1809359/1‑69 (MQ=255) aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat < 1:507235/44‑1 (MQ=255) aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat > 1:3011819/1‑44 (MQ=255) aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat > 1:523404/1‑44 (MQ=255) aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat > 1:569366/1‑44 (MQ=255) aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat < 1:1585738/44‑1 (MQ=255) aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat < 1:1556332/44‑1 (MQ=255) aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat > 1:1510743/1‑44 (MQ=255) aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat < 1:1334456/44‑1 (MQ=255) aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat < 1:1290739/44‑1 (MQ=255) aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat > 1:1218396/1‑44 (MQ=255) aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat > 1:912222/1‑44 (MQ=255) aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat < 1:1855416/44‑1 (MQ=255) aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat > 1:503457/1‑44 (MQ=255) aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat > 1:3010831/1‑44 (MQ=255) aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat < 1:1924926/44‑1 (MQ=255) aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat > 1:1985489/1‑44 (MQ=255) aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat > 1:2243773/1‑44 (MQ=255) aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat > 1:2267672/1‑44 (MQ=255) aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat > 1:3013022/1‑44 (MQ=255) aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat > 1:2429069/1‑44 (MQ=255) aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat < 1:2814541/44‑1 (MQ=255) aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat > 1:289695/1‑44 (MQ=255) aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCACGCCCGCATAGATg > 1:941002/1‑69 (MQ=255) aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCACGCCCGCATAGATg > 1:2908817/1‑69 (MQ=255) aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCACGCCCGCATAGATg > 1:1354287/1‑69 (MQ=255) aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCACGCCCGCATAGATg > 1:2393651/1‑69 (MQ=255) aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCACGCCCGCATAGATg > 1:1911502/1‑69 (MQ=255) aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAAATGGGTGATGATTTCCGTCACGCCCGCATAGATg > 1:601604/1‑69 (MQ=255) | ACAATCGCCACGCCGCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCACGCCCGCATAGATG > minE/1251024‑1251152 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |