| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,251,528 | A→G | 35.9% | G180G (GGT→GGC) | yebC ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,251,528 | 0 | A | G | 35.9% | 37.9 / 37.1 | 64 | G180G (GGT→GGC) | yebC | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (16/25); new base G (8/15); total (24/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
GACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCAC > minE/1251460‑1251595 | gACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTa < 1:338537/69‑1 (MQ=255)gACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTa < 1:3063806/69‑1 (MQ=255) ggATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCa < 1:879880/69‑1 (MQ=255) ggATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCa < 1:3383772/69‑1 (MQ=255) cgcTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAgtg < 1:2534670/68‑1 (MQ=255) cgcTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAgtg < 1:2865285/68‑1 (MQ=255) cgcTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAgtg < 1:3111216/68‑1 (MQ=255) gcTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTa < 1:2298594/69‑1 (MQ=255) gcTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTa < 1:2081944/69‑1 (MQ=255) gcTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTa < 1:87254/69‑1 (MQ=255) gcTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTa < 1:2736017/69‑1 (MQ=255) gcTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTa < 1:583437/69‑1 (MQ=255) tGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAg > 1:2674528/1‑59 (MQ=255) tGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATc > 1:807979/1‑69 (MQ=255) gCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTa < 1:2345162/39‑1 (MQ=255) tttCAGACCTGCCGCTTCCAGAGCGTCGCGTACTTTGccc > 1:1092780/1‑40 (MQ=38) tttCAGACCTGCCGCTTCCAGAGCGTCGCGTACTTTGCCCATttctt > 1:1408420/1‑47 (MQ=255) tttCAGACCTGCCGCTTCCAGAGCGTCGCGTACTTTGCCCATTTCTTCCCATGCAGTGTAGAcct > 1:1408833/1‑63 (MQ=255) tttCAGACCTGCCGCTTCCAGAGCGTCGCGTACTTTGCCCATTTCTTCCCATGCAGTGTAGACATCaa > 1:2880214/1‑68 (MQ=255) tttCAGACCTGCCGCTTCCAGAGCGTCGCGTACTTTGCCCATTTCTTCCCATGCAGTGTAGACATCaa > 1:1052656/1‑68 (MQ=255) tttCAGACCTGCCGCTTCCAGAGCGTCGCGTACTTTGCCCATTTCTTCCCATGCAGTGTAGACATCAAt > 1:26185/1‑69 (MQ=255) tttCAGACCTGCCGCTTCCAGAGCGTCGCGTACTTTGCCCATTTCTTCCCATGCAGGGTAGAc > 1:2921879/1‑63 (MQ=255) tttCAGACCTGCCGCTTCCAGAGCGTCGCGTACGTTGCCCATTTCTTCCCATGCAGTGTAGACATCaa > 1:1163359/1‑68 (MQ=255) cTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATg > 1:2718526/1‑45 (MQ=255) cTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGt > 1:501927/1‑69 (MQ=255) cTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGt > 1:60638/1‑69 (MQ=255) cTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGt > 1:2610219/1‑69 (MQ=255) cTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGt > 1:241210/1‑69 (MQ=255) cTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGt > 1:956321/1‑69 (MQ=255) cTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGt > 1:2524176/1‑69 (MQ=255) cTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGt > 1:2250937/1‑69 (MQ=255) cTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGt > 1:2132232/1‑69 (MQ=255) cTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGt > 1:2110294/1‑69 (MQ=255) cTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGt > 1:2087811/1‑69 (MQ=255) cTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGt > 1:2081628/1‑69 (MQ=255) cTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCAGt > 1:1248512/1‑69 (MQ=255) gCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGtcat < 1:2295911/67‑1 (MQ=255) gCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGtcat < 1:1443770/67‑1 (MQ=255) gCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGtcat < 1:1030361/67‑1 (MQ=255) gCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGtcat < 1:42656/67‑1 (MQ=255) gcgtACTTTGCCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTc < 1:1240391/69‑1 (MQ=255) gcgtACTTTGCCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTc < 1:3269824/69‑1 (MQ=255) gcgtACTTTGCCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTc < 1:3381606/69‑1 (MQ=255) gcgtACTTTGCCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTc < 1:1395195/69‑1 (MQ=255) gcgtACTTTGCCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTc < 1:1372615/69‑1 (MQ=255) gcgtACTTTGCCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTc < 1:3092190/69‑1 (MQ=255) gcgtACTTTGCCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTc < 1:1231909/69‑1 (MQ=255) gcgtACTTTGCCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTc < 1:10712/69‑1 (MQ=255) gcgtACTTTGCCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTc < 1:1038149/69‑1 (MQ=255) gcgtACTTTGCCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTc < 1:2929102/69‑1 (MQ=255) gcgtACTTTGCCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTc < 1:19721/69‑1 (MQ=255) gcgtACTTTGCCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTc < 1:2002638/69‑1 (MQ=255) gcgtACTTTGCCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTc < 1:2005791/69‑1 (MQ=255) gcgtACTTTGCCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTc < 1:1569999/69‑1 (MQ=255) gcgtACTTTGCCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTc < 1:2661246/69‑1 (MQ=255) cTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAg < 1:619686/69‑1 (MQ=255) tttACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGc < 1:2445113/69‑1 (MQ=255) tttACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGc < 1:2501895/69‑1 (MQ=255) tttACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGc < 1:1226468/69‑1 (MQ=255) tttACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGc < 1:633933/69‑1 (MQ=255) tttACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGc < 1:2686211/69‑1 (MQ=255) tttACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGc < 1:1307350/69‑1 (MQ=255) tttACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGc < 1:2743985/69‑1 (MQ=255) tttACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGc < 1:355078/69‑1 (MQ=255) tttACCCATTTCTTCCCATGCAGTGTAGACATCAATCGAGCCGTCATCATAGGTCACAACGTCTTCAGc < 1:533275/69‑1 (MQ=255) ttACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGCCTTCAGc < 1:934182/68‑1 (MQ=255) tACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCAc > 1:2048217/1‑69 (MQ=255) | GACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCAC > minE/1251460‑1251595 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |