| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 102,711 | G→A | 65.0% | A225A (GCC→GCT) | nadC ← | quinolinate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 102,711 | 0 | G | A | 65.0% | 34.5 / 31.3 | 57 | A225A (GCC→GCT) | nadC | quinolinate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (9/11); new base A (20/17); total (29/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.79e-01 | |||||||||||
TGACGGCTTCGCGCATCTGTTCTGTTTCGAAGTTATCCAGCATGATGATATCGGCTCCTGCTTTCAGGGCTTCATCAAGTTCTTCCAGATTCTCTACTTCGACTTCTACTGGCGCATCCGGGTGCA > minE/102644‑102769 | tGACGGCTTCGCGCATCTGTTCTGTTTCGAAGTTATCCAGCATGATGATATCGGCTCCTGCTTTCAggg > 1:1092775/1‑69 (MQ=255)tGACGGCTTCGCGCATCTGTTCTGTTTCGAAGTTATCCAGCATGATGATATCGGCTCCTGCTTTCAggg > 1:1407089/1‑69 (MQ=255)tGACGGCTTCGCGCATCTGTTCTGTTTCGAAGTTATCCAGCATGATGATATCGGCTCCTGCTTTCAggg > 1:643251/1‑69 (MQ=255)tGACGGCTTCGCGCATCTGTTCTGTTTCGAAGTTATCCAGCATGATGATATCGGCTCCTGCTTTCAggg > 1:2107739/1‑69 (MQ=255) ggCTTCGCGCATCTGTTCTGTTTCGAAGTTATCCAGCATGATGATATCGGCACCTGCTTTCAGAGc > 1:3042588/1‑66 (MQ=255) ggCTTCGCGCATCTGTTCTGTTTCGAAGTTATCCAGCATGATGATATCGGCACCTGCTTTCAGAGc > 1:142527/1‑66 (MQ=255) ggCTTCGCGCATCTGTTCTGTTTCGAAGTTATCCAGCATGATGATATCGGCACCTGCTTTCAGAGc > 1:1794645/1‑66 (MQ=255) ggCTTCGCGCATCTGTTCTGTTTCGAAGTTATCCAGCATGATGATATCGGCACCTGCTTTCAGAGc > 1:264304/1‑66 (MQ=255) ggCTTCGCGCATCTGTTCTGTTTCGAAGTTATCCAGCATGATGATATCGGCACCTGCTTTCAGAGCt > 1:3311228/1‑67 (MQ=255) ggCTTCGCGCATCTGTTCTGTTTCGAAGTTATCCAGCATGATGATATCGGCACCTGCTTTCAGAGCt > 1:2499158/1‑67 (MQ=255) ggCTTCGCGCATCTGTTCTGTTTCGAAGTTATCCAGCATGATGATATCGGCACCTGCTTTCAGAGCt > 1:2950090/1‑67 (MQ=255) ggCTTCGCGCATCTGTTCTGTTTCGAAGTTATCCAGCATGATGATATCGGCACCTGCTTTCAGAGCt > 1:3033016/1‑67 (MQ=255) ggCTTCGCGCATCTGTTCTGTTTCGAAGTTATCCAGCATGATGATATCGGCACCTGCTTTCAGAGCt > 1:2334136/1‑67 (MQ=255) ggCTTCGCGCATCTGTTCTGTTTCGAAGTTATCCAGCATGATGATATCGGCACCTGCTTTCAGAGCt > 1:2285695/1‑67 (MQ=255) ggCTTCGCGCATCTGTTCTGTTTCGAAGTTATCCAGCATGATGATATCGGCACCTGCTTTCAGAGCt > 1:2631896/1‑67 (MQ=255) ggCTTCGCGCATCTGTTCTGTTTCGAAGTTATCCAGCATGATGATATCGGCACCTGCTTTCAGAGCt > 1:2912752/1‑67 (MQ=255) ggCTTCGCGCATCTGTTCTGTTTCGAAGTTATCCAGCATGATGATATCGGCACCTGCTTTCAGAGCt > 1:3301647/1‑67 (MQ=255) ggCTTCGCGCATCTGTTCTGTTTCGAAGTTATCCAGCATGATGATATCGGCACCTGCTTTCAGAGCt > 1:3330817/1‑67 (MQ=255) ggCTTCGCGCATCTGTTCTGTTTCGAAGTTATCCAGCATGATGATATCGGCACCTGCTTTCAGAGCt > 1:1401816/1‑67 (MQ=255) ggCTTCGCGCATCTGTTCTGTTTCGAAGTTATCCAGCATGATGATATCGGCACCTGCTTTCAGAGCt > 1:1368616/1‑67 (MQ=255) ggCTTCGCGCATCTGTTCTGTTTCGAAGTTATCCAGCATGATGATATCGGCACCTGCTTTCAGAGCt > 1:802112/1‑67 (MQ=255) ggCTTCGCGCATCTGTTCTGTTTCGAAGTTATCCAGCATGATGATATCGGCACCTGCTTTCAGAGCt > 1:1291782/1‑67 (MQ=255) ggCTTCGCGCATCTGTTCTGTTTCGAAGTTATCCAGCATGATGATATCGGCACCTGCTTTCAGAGCt > 1:842958/1‑67 (MQ=255) ggCTTCGCGCATCTGTTCTGTTTCGAAGTTATCCAGCATGATGATATCGGCACCTGCTTTCAGAGCt > 1:2859802/1‑67 (MQ=255) gCTTCGCGCATCTGTTCTGTTTCGAAGTTATCCAGCATGATGATATCGGCTCCTGCTTTCAGGGCTtc > 1:1006572/1‑68 (MQ=255) gCTTCGCGCATCTGTTCTGTTTCGAAGTTATCCAGCATGATGATATCGGCTCCTGCTTTCAGGGCTtc > 1:339175/1‑68 (MQ=255) tCGAAGTTATCCAGCATGATGATATCGGCTCCTGCTTTCAGGGCTTCATCAAGTTCTTCCAGATTctct > 1:1036351/1‑69 (MQ=255) tCGAAGTTATCCAGCATGATGATATCGGCTCCTGCTTTCAGGGCTTCATCAAGTTCTTCCAGATTctct > 1:3260822/1‑69 (MQ=255) atgatATCGGCTCCTGCTTTCAGGGCTTCATCAAGttcttc > 1:2073767/1‑41 (MQ=255) atgatATCGGCTCCTGCTTTCAGGGCTTCATCAAGTTCTTCCAGATTCTCTACTTCGACTTCTACTgg > 1:3010607/1‑68 (MQ=255) atgatATCGGCTCCTGCTTTCAGGGCTTCATCAAGTTCTTCCAGATTCTCTACTTCGACTTCTACTgg > 1:207933/1‑68 (MQ=255) tgatATCGGCTCCTGCTTTCAGGGCTTCATCAAGTTCTTCCAGATtctc < 1:3182138/49‑1 (MQ=255) tgatATCGGCTCCTGCTTTCAGGGCTTCATCAAGTTCTTCCAGATtctc < 1:2880085/49‑1 (MQ=255) tgatATCGGCTCCTGCTTTCAGGGCTTCATCAAGTTCTTCCAGATtctc < 1:2633350/49‑1 (MQ=255) tgatATCGGCTCCTGCTTTCAGGGCTTCATCAAGTTCTTCCAGATtctc < 1:2677466/49‑1 (MQ=255) tgatATCGGCTCCTGCTTTCAGGGCTTCATCAAGTTCTTCCAGATtctc < 1:22194/49‑1 (MQ=255) tgatATCGGCTCCTGCTTTCAGGGCTTCATCAAGTTCTTACAGATtctc < 1:1045994/49‑1 (MQ=255) tgatATCGGCACCTGCTTTCAGAGCTTCATCAAGTTCTTCCAGATTCTCTACTTCGACTTCt < 1:3105546/62‑1 (MQ=255) tgatATCGGCACCTGCTTTCAGAGCTTCATCAAGTTCTTCCAGATTCTCTACTTCGACTTCt < 1:351694/62‑1 (MQ=255) tgatATCGGCACCTGCTTTCAGAGCTTCATCAAGTTCTTCCAGATTCTCTACTTCGACTTCt < 1:3422210/62‑1 (MQ=255) tgatATCGGCACCTGCTTTCAGAGCTTCATCAAGTTCTTCCAGATTCTCTACTTCGACTTCt < 1:164415/62‑1 (MQ=255) tgatATCGGCACCTGCTTTCAGAGCTTCATCAAGTTCTTCCAGATTCTCTACTTCGACTTCt < 1:1728830/62‑1 (MQ=255) tgatATCGGCACCTGCTTTCAGAGCTTCATCAAGTTCTTCCAGATTCTCTACTTCGACTTCt < 1:174601/62‑1 (MQ=255) tgatATCGGCACCTGCTTTCAGAGCTTCATCAAGTTCTTCCAGATTCTCTACTTCGACTTCt < 1:1634697/62‑1 (MQ=255) tgatATCGGCACCTGCTTTCAGAGCTTCATCAAGTTCTTCCAGATTCTCTACTTCGACTTCt < 1:2003641/62‑1 (MQ=255) tgatATCGGCACCTGCTTTCAGAGCTTCATCAAGTTCTTCCAGATTCTCTACTTCGACTTCt < 1:3194332/62‑1 (MQ=255) tgatATCGGCACCTGCTTTCAGAGCTTCATCAAGTTCTTCCAGATTCTCTACTTCGACTTCt < 1:3127646/62‑1 (MQ=255) tgatATCGGCACCTGCTTTCAGAGCTTCATCAAGTTCTTCCAGATTCTCTACTTCGACTTCt < 1:2417955/62‑1 (MQ=255) tgatATCGGCACCTGCTTTCAGAGCTTCATCAAGTTCTTCCAGATTCTCTACTTCGACTTCt < 1:2500065/62‑1 (MQ=255) tgatATCGGCACCTGCTTTCAGAGCTTCATCAAGTTCTTCCAGATTCTCTACTTCGACTTCt < 1:2632374/62‑1 (MQ=255) tgatATCGGCACCTGCTTTCAGAGCTTCATCAAGTTCTTCCAGATTCTCTACTTCGACTTCt < 1:689815/62‑1 (MQ=255) tgatATCGGCACCTGCTTTCAGAGCTTCATCAAGTTCTTCCAGATTCTCTACTTCGACTTCt < 1:2835951/62‑1 (MQ=255) tgatATAGGCACCTGCTTTCAGAGCTTCATCAAGTTCTTCCAGATTCTCTACTTCGACTTCt < 1:2302890/62‑1 (MQ=255) gatATCGGCTCCTGCTTTCAGGGCTTCATCAAGTTCTTCCAGATTCTCTACTTCGACTTCt > 1:2482899/1‑61 (MQ=255) atatCGGCTCCTGCTTTCAGGGCTTCATCAAGTTCTTCCAGATTCTCTACTTCGACTTCTACTGGCGCa < 1:897189/69‑1 (MQ=255) atatCGGCACCTGCTTTCAGAGCTTCATCAAGTTCTTCCAGATTCTCTACTTCGACTTCt < 1:1872950/60‑1 (MQ=255) tatCGGCTCCTGCTTTCAGGGCTTCATCAAGTTCTTCCAGATTCTCTACTTCGACTTCTACTGGCGCa < 1:861357/68‑1 (MQ=255) cGGCTCCTGCTTTCAGGGCTTCATCAAGTTCTTCCAGATTCTCTACTTCg < 1:2844792/50‑1 (MQ=255) ggCTCCTGCTTTCAGGGCTTCATCAAGTTCTTCCAGATTCTCTa < 1:129749/44‑1 (MQ=255) ggCTCCTGCTTTCAGGGCTTCATCAAGTTCTTCCAGATTCTCTa < 1:2870147/44‑1 (MQ=255) gCTTTCAGGGCTTCATCAAGTTCTTCCAGATTCTCTACTTCGACTTCTACTGGCGCATCCGGGTGCa > 1:265574/1‑67 (MQ=255) | TGACGGCTTCGCGCATCTGTTCTGTTTCGAAGTTATCCAGCATGATGATATCGGCTCCTGCTTTCAGGGCTTCATCAAGTTCTTCCAGATTCTCTACTTCGACTTCTACTGGCGCATCCGGGTGCA > minE/102644‑102769 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |