| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,257,700 | C→A | 100% | L115M (CTG→ATG) | yecP → | predicted methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,257,700 | 0 | C | A | 81.8% | 33.0 / 3.7 | 22 | L115M (CTG→ATG) | yecP | predicted methyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (1/3); new base A (0/18); total (1/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTAT > minE/1257655‑1257762 | aaCATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAAc > 1:217741/1‑68 (MQ=255) gAATGGCGTTCCGACTGGAAATGGGATCGCGTTATGcccc < 1:296644/40‑1 (MQ=38) gAATGGCGTTCCGACTGGAAATGGGATCGCGTTATGcccc < 1:96582/40‑1 (MQ=38) gAATGGCGTTCCGACTGGAAATGGGATCGCGTTATGcccc < 1:608964/40‑1 (MQ=38) gAATGGCGTTCCGACTGGAAATGGGATCGCGTTATGcccc < 1:506/40‑1 (MQ=38) gAATGGCGTTCCGACTGGAAATGGGATCGCGTTATGcccc < 1:47655/40‑1 (MQ=38) gAATGGCGTTCCGACTGGAAATGGGATCGCGTTATGcccc < 1:469276/40‑1 (MQ=38) gAATGGCGTTCCGACTGGAAATGGGATCGCGTTATGcccc < 1:3394937/40‑1 (MQ=38) gAATGGCGTTCCGACTGGAAATGGGATCGCGTTATGcccc < 1:3005006/40‑1 (MQ=38) gAATGGCGTTCCGACTGGAAATGGGATCGCGTTATGcccc < 1:2833515/40‑1 (MQ=38) gAATGGCGTTCCGACTGGAAATGGGATCGCGTTATGcccc < 1:2691858/40‑1 (MQ=38) gAATGGCGTTCCGACTGGAAATGGGATCGCGTTATGcccc < 1:2646831/40‑1 (MQ=38) gAATGGCGTTCCGACTGGAAATGGGATCGCGTTATGcccc < 1:2526130/40‑1 (MQ=38) gAATGGCGTTCCGACTGGAAATGGGATCGCGTTATGcccc < 1:2426528/40‑1 (MQ=38) gAATGGCGTTCCGACTGGAAATGGGATCGCGTTATGcccc < 1:1796785/40‑1 (MQ=38) gAATGGCGTTCCGACTGGAAATGGGATCGCGTTATGcccc < 1:1752318/40‑1 (MQ=38) gAATGGCGTTCCGACTGGAAATGGGATCGCGTTATGcccc < 1:1713079/40‑1 (MQ=38) gAATGGCGTTCCGACTGGAAATGGGATCGCGTTATGcccc < 1:1482627/40‑1 (MQ=38) aaTGGCGTTCCGACTGGAAATGGGATCGCGTTATGcccc < 1:1148573/39‑1 (MQ=38) tGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTg < 1:494082/69‑1 (MQ=255) cGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTAt < 1:3376310/69‑1 (MQ=255) cGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTAt < 1:494254/69‑1 (MQ=255) | AACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTAT > minE/1257655‑1257762 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |