| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,258,176 | G→A | 65.5% | A273A (GCG→GCA) | yecP → | predicted methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,258,176 | 0 | G | A | 65.5% | 54.4 / 45.5 | 81 | A273A (GCG→GCA) | yecP | predicted methyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (15/13); new base A (28/25); total (43/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 104 total aligned reads displayed.
TCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAA > minE/1258109‑1258233 | tCATCCCTTCCGCGCTGTCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCg > 1:1144114/1‑68 (MQ=255)tCATCCCTTCCGCGCTGTCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCg > 1:1148431/1‑68 (MQ=255)tCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCgg > 1:841307/1‑69 (MQ=255)tCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCg > 1:2211769/1‑68 (MQ=255)tCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCg < 1:578358/68‑1 (MQ=255)tCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCg > 1:3267042/1‑68 (MQ=255)tCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCg < 1:1663097/68‑1 (MQ=255)tCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCg > 1:1749325/1‑68 (MQ=255)tCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCg < 1:1751468/68‑1 (MQ=255)tCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCg < 1:2186316/68‑1 (MQ=255)tCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCg > 1:2208951/1‑68 (MQ=255)tCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCg < 1:954042/68‑1 (MQ=255)tCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCg > 1:2872170/1‑68 (MQ=255)tCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCg < 1:2332625/68‑1 (MQ=255)tCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCg < 1:246976/68‑1 (MQ=255)tCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCg > 1:2731581/1‑68 (MQ=255)tCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCg < 1:2622416/68‑1 (MQ=255)tCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCg > 1:916352/1‑68 (MQ=255)tCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCg > 1:1189946/1‑68 (MQ=255)tCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCg < 1:1254615/68‑1 (MQ=255)tCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCg > 1:1355772/1‑68 (MQ=255)tCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCg < 1:141680/68‑1 (MQ=255) cccTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGAt > 1:2701633/1‑67 (MQ=255) cccTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGAt > 1:2447113/1‑67 (MQ=255) cccTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGAt > 1:2186673/1‑67 (MQ=255) cccTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGAt > 1:291224/1‑67 (MQ=255) cccTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGAt > 1:647966/1‑67 (MQ=255) cccTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGAt > 1:1574141/1‑67 (MQ=255) cccTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGAt > 1:1574441/1‑67 (MQ=255) ccTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTT‑TAGATATCCGCATTGCGGATGTGa > 1:1231278/1‑69 (MQ=255) ggcgctgAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTaccac < 1:3043737/69‑1 (MQ=255) ggcgctgAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTaccac < 1:1616151/69‑1 (MQ=255) tgAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTTCCACCACag < 1:1164376/69‑1 (MQ=255) tgAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACag < 1:594581/69‑1 (MQ=255) tgAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACag < 1:2344461/69‑1 (MQ=255) tgAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACag < 1:45969/69‑1 (MQ=255) tgAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACag < 1:1001838/69‑1 (MQ=255) tgAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACag < 1:2599921/69‑1 (MQ=255) tgAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACag < 1:2708783/69‑1 (MQ=255) aaaCTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACagaag < 1:280878/68‑1 (MQ=255) aaaCTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACagaag < 1:2615551/68‑1 (MQ=255) gaagTGTGGTTTTGTTGATATTCGCATTGCAGAtgt > 1:2535987/1‑36 (MQ=25) gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGa > 1:913293/1‑38 (MQ=25) gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg > 1:508194/1‑39 (MQ=25) gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg > 1:2856284/1‑39 (MQ=25) gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg > 1:3368350/1‑39 (MQ=25) gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg > 1:916260/1‑39 (MQ=25) gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg > 1:2855889/1‑39 (MQ=25) gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg > 1:2953776/1‑39 (MQ=25) gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg > 1:301624/1‑39 (MQ=25) gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg > 1:3034774/1‑39 (MQ=25) gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg > 1:3067803/1‑39 (MQ=25) gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg > 1:3071854/1‑39 (MQ=25) gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg > 1:867771/1‑39 (MQ=25) gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg > 1:2560424/1‑39 (MQ=25) gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg > 1:3309967/1‑39 (MQ=25) gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg > 1:2702561/1‑39 (MQ=25) gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg > 1:3158133/1‑39 (MQ=25) gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg > 1:1034629/1‑39 (MQ=25) gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg > 1:1622627/1‑39 (MQ=25) gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg > 1:1766674/1‑39 (MQ=25) gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg > 1:1794134/1‑39 (MQ=25) gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg > 1:1566073/1‑39 (MQ=25) gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg > 1:1993413/1‑39 (MQ=25) gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg > 1:1137297/1‑39 (MQ=25) gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg > 1:2327393/1‑39 (MQ=25) gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg > 1:2356394/1‑39 (MQ=25) gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg > 1:253146/1‑39 (MQ=25) gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg > 1:2605234/1‑39 (MQ=25) gtgGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGagc > 1:1817635/1‑54 (MQ=255) gtgGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGaa > 1:1943760/1‑68 (MQ=255) tgGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAatg < 1:549819/69‑1 (MQ=255) tatCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCg < 1:3255424/68‑1 (MQ=255) atCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGaa > 1:126385/1‑69 (MQ=255) atCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGaa > 1:932661/1‑69 (MQ=255) atCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGaa > 1:3376728/1‑69 (MQ=255) atCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGaa > 1:1738407/1‑69 (MQ=255) tGCAGATGTGAGCGTTACCACGACAGAAGAGCAGCGACGCACCGAATGGATGGt < 1:2695153/54‑1 (MQ=255) tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCTAATGGATGGt < 1:483190/54‑1 (MQ=255) tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt < 1:1502546/54‑1 (MQ=255) tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt < 1:2680982/54‑1 (MQ=255) tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt < 1:2534152/54‑1 (MQ=255) tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt < 1:1049225/54‑1 (MQ=255) tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt < 1:1088630/54‑1 (MQ=255) tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt < 1:2883288/54‑1 (MQ=255) tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt < 1:895612/54‑1 (MQ=255) tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt < 1:2051549/54‑1 (MQ=255) tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt < 1:1375008/54‑1 (MQ=255) tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt < 1:759638/54‑1 (MQ=255) tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt < 1:686964/54‑1 (MQ=255) tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt < 1:1425301/54‑1 (MQ=255) tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt < 1:1643787/54‑1 (MQ=255) tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt < 1:1856911/54‑1 (MQ=255) tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt < 1:3134164/54‑1 (MQ=255) tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt < 1:1579243/54‑1 (MQ=255) tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt < 1:475263/54‑1 (MQ=255) tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt < 1:409286/54‑1 (MQ=255) tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt < 1:3383082/54‑1 (MQ=255) tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt < 1:1633192/54‑1 (MQ=255) tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt < 1:1638725/54‑1 (MQ=255) | TCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAA > minE/1258109‑1258233 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |