Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 103,122 T→A 43.2% I88I (ATA→ATT nadC ← quinolinate phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE103,1220TA43.2% 32.1 / 100.9 95I88I (ATA→ATTnadCquinolinate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (25/29);  new base A (22/19);  total (47/48)
Fisher's exact test for biased strand distribution p-value = 5.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01

Only 100 of 101 total aligned reads displayed.

CCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGCGTTTGCCGCAAAAGACGCC  >  minE/103060‑103190
                                                              |                                                                    
ccTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGt                                                                 <  1:356879/68‑1 (MQ=255)
ccTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGt                                                                 <  1:122072/68‑1 (MQ=255)
    cAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGt                                                                 <  1:2842231/64‑1 (MQ=255)
    cAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGt                                                                 <  1:2096737/64‑1 (MQ=255)
    cAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGt                                                                 <  1:3293792/64‑1 (MQ=255)
    cAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGt                                                                 <  1:140393/64‑1 (MQ=255)
    cAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGt                                                                 <  1:2435450/64‑1 (MQ=255)
    cAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGt                                                                 <  1:2409515/64‑1 (MQ=255)
    cAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGt                                                                 <  1:2801182/64‑1 (MQ=255)
    cAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCACATTATGGt                                                                 <  1:1249711/64‑1 (MQ=255)
       gTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACAtcgt                                                         <  1:1158975/69‑1 (MQ=255)
       gTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACAtcgt                                                         <  1:763343/69‑1 (MQ=255)
       gTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACAtcgt                                                         <  1:2467184/69‑1 (MQ=255)
       gTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACAtcgt                                                         <  1:1980224/69‑1 (MQ=255)
        ttCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACAtcgtc                                                        <  1:2086383/69‑1 (MQ=255)
         tCGAACATGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACAtcgtcg                                                       <  1:2514498/69‑1 (MQ=255)
         tCGAACATGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACAtcgtcg                                                       <  1:1546108/69‑1 (MQ=255)
         tCGAACATGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACAtcgtcg                                                       <  1:2982906/69‑1 (MQ=255)
         tCGAACATGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACAtcgtcg                                                       <  1:2282298/69‑1 (MQ=255)
         tCGAACATGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACAtcgtcg                                                       <  1:1290243/69‑1 (MQ=255)
         tCGAACATGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACAtcgtcg                                                       <  1:959637/69‑1 (MQ=255)
         tCGAACAAGGGTTGATTGGCGTTGATGACATCGCCGTCATCCACATGCCAGATAATGGTGACAtcgtcg                                                       >  1:1001271/1‑69 (MQ=255)
         tCGAACAAGGGTTGATTGGCGTTGATGACATCGCCGTCATCCACATGCCAGATAATGGTGACAtcgtcg                                                       >  1:245137/1‑69 (MQ=255)
         tCGAACAAGGGTTGATTGGCGTTGATGACATCGCCGTCATCCACATGCCAGATAATGGTGACAtcgtcg                                                       >  1:2469605/1‑69 (MQ=255)
         tCGAACAAGGGTTGATTGGCGTTGATGACATCGCCGTCATCCACATGCCAGATAATGGTGACAtcgtcg                                                       >  1:2264244/1‑69 (MQ=255)
         tCGAACAAGGGTTGATTGGCGTTGATGACATCGCCGTCATCCACATGCCAGATAATGGTGACAtcgtcg                                                       >  1:2500175/1‑69 (MQ=255)
         tCGAACAAGGGTTGATTGGCGTTGATGACATCGCCGTCATCCACATGCCAGATAATGGTGACAtcgtcg                                                       >  1:2570629/1‑69 (MQ=255)
         tCGAACAAGGGTTGATTGGCGTTGATGACATCGCCGTCATCCACATGCCAGATAATGGTGACAtcgtcg                                                       >  1:2755999/1‑69 (MQ=255)
         tCGAACAAGGGTTGATTGGCGTTGATGACATCGCCGTCATCCACATGCCAGATAATGGTGACAtcgtcg                                                       >  1:2771463/1‑69 (MQ=255)
         tCGAACAAGGGTTGATTGGCGTTGATGACATCGCCGTCATCCACATGCCAGATAATGGTGACAtcgtcg                                                       >  1:2914980/1‑69 (MQ=255)
         tCGAACAAGGGTTGATTGGCGTTGATGACATCGCCGTCATCCACATGCCAGATAATGGTGACAtcgtcg                                                       >  1:2976551/1‑69 (MQ=255)
         tCGAACAAGGGTTGATTGGCGTTGATGACATCGCCGTCATCCACATGCCAGATAATGGTGACAtcgtcg                                                       >  1:3007361/1‑69 (MQ=255)
         tCGAACAAGGGTTGATTGGCGTTGATGACATCGCCGTCATCCACATGCCAGATAATGGTGACAtcgtcg                                                       >  1:379246/1‑69 (MQ=255)
         tCGAACAAGGGTTGATTGGCGTTGATGACATCGCCGTCATCCACATGCCAGATAATGGTGACAtcgtcg                                                       >  1:462045/1‑69 (MQ=255)
         tCGAACAAGGGTTGATTGGCGTTGATGACATCGCCGTCATCCACATGCCAGATAATGGTGACAtcgtcg                                                       >  1:634326/1‑69 (MQ=255)
         tCGAACAAGGGTTGATTGGCGTTGATGACATCGCCGTCATCCACATGCCAGATAATGGTGACAtcgtcg                                                       >  1:922088/1‑69 (MQ=255)
         tCGAACAAGGGTTGATTGGCGTTGATGACATCGCCGTCATCCACATGCCAGATAATGGTGACAtcgtcg                                                       >  1:102118/1‑69 (MQ=255)
         tCGAACAAGGGTTGATTGGCGTTGATGACATCGCCGTCATCCACATGCCAGATAATGGTGACAtcgtcg                                                       >  1:1451302/1‑69 (MQ=255)
         tCGAACAAGGGTTGATTGGCGTTGATGACATCGCCGTCATCCACATGCCAGATAATGGTGACAtcgtcg                                                       >  1:2208753/1‑69 (MQ=255)
         tCGAACAAGGGTTGATTGGCGTTGATGACATCGCCGTCATCCACATGCCAGATAATGGTGACAtcgtcg                                                       >  1:1780937/1‑69 (MQ=255)
         tCGAACAAGGGTTGATTGGCGTTGATGACATCGCCGTCATCCACATGCCAGATAATGGTGACAtcgtcg                                                       >  1:1124264/1‑69 (MQ=255)
         tCGAACAAGGGTTGATTGGCGTTGATGACATCGCCGTCATCCACATGCCAGATAATGGTGACAtcgtc                                                        >  1:2448606/1‑68 (MQ=255)
         tCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACAtcgtcg                                                       <  1:805932/69‑1 (MQ=255)
               aaGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGt                                                                 <  1:1953641/53‑1 (MQ=255)
                      gattgGCATTGATGACATCGCCGTCTTCCACATGCCAGATTATGGTGACAtcgt                                                         <  1:2195872/54‑1 (MQ=255)
                         tgGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATa                                        <  1:3414921/68‑1 (MQ=255)
                                         gCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCa                       >  1:600197/1‑69 (MQ=255)
                                         gCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCa                       >  1:898159/1‑69 (MQ=255)
                                            gTCATCCACATGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGCg                    <  1:2291988/69‑1 (MQ=255)
                                              cATCCACATGCCAGATAATGGTGACATCGTCGCCTGCCAGTTGAATAAACCCCTCTTCAACCCAGCGtt                  <  1:431838/69‑1 (MQ=255)
                                              cATCCACATGCCAGATAATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGCGtt                  <  1:1521359/69‑1 (MQ=255)
                                              cATCCACATGCCAGATAATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGCGtt                  <  1:3030136/69‑1 (MQ=255)
                                              cATCCACATGCCAGATAATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGCGtt                  <  1:3102428/69‑1 (MQ=255)
                                              cATCCACATGCCAGATAATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGCGtt                  <  1:3211607/69‑1 (MQ=255)
                                              cATCCACATGCCAGATAATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGCGtt                  <  1:179484/69‑1 (MQ=255)
                                              cATCCACATGCCAGATAATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGCGtt                  <  1:3282705/69‑1 (MQ=255)
                                              cATCCACATGCCAGATAATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGCGtt                  <  1:3332127/69‑1 (MQ=255)
                                              cATCCACATGCCAGATAATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGCGtt                  <  1:3390796/69‑1 (MQ=255)
                                              cATCCACATGCCAGATAATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGCGtt                  <  1:1425426/69‑1 (MQ=255)
                                              cATCCACATGCCAGATAATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGCGtt                  <  1:401642/69‑1 (MQ=255)
                                              cATCCACATGCCAGATAATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGCGtt                  <  1:934272/69‑1 (MQ=255)
                                              cATCCACATGCCAGATAATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGCGtt                  <  1:2228151/69‑1 (MQ=255)
                                              cATCCACATGCCAGATAATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGCGtt                  <  1:1960767/69‑1 (MQ=255)
                                              cATCCACATGCCAGATAATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGCGtt                  <  1:2069940/69‑1 (MQ=255)
                                              cATCCACATGCCAGATAATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGCGtt                  <  1:2602790/69‑1 (MQ=255)
                                              cATCCACATGCCAGATAATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGCGtt                  <  1:2150886/69‑1 (MQ=255)
                                              cATCCACATGCCAGATAATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGCGtt                  <  1:2411314/69‑1 (MQ=255)
                                                  cacaTGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATa                                        >  1:2026850/1‑43 (MQ=255)
                                                  cacaTGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATa                                        >  1:2263902/1‑43 (MQ=255)
                                                  cacaTGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATa                                        >  1:2124640/1‑43 (MQ=255)
                                                  cacaTGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATa                                        >  1:2445734/1‑43 (MQ=255)
                                                  cacaTGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATa                                        >  1:582638/1‑43 (MQ=255)
                                                  cacaTGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATa                                        >  1:1328186/1‑43 (MQ=255)
                                                  cacaTGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATa                                        >  1:240386/1‑43 (MQ=255)
                                                  cacaTGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATa                                        >  1:766189/1‑43 (MQ=255)
                                                  cacaTGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATa                                        >  1:824856/1‑43 (MQ=255)
                                                  cacaTGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATa                                        >  1:227197/1‑43 (MQ=255)
                                                  cacaTGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATa                                        >  1:1123433/1‑43 (MQ=255)
                                                  cacaTGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATa                                        >  1:1810387/1‑43 (MQ=255)
                                                  cacaTGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATa                                        <  1:2697410/43‑1 (MQ=255)
                                                  cacaTGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATa                                        <  1:3180528/43‑1 (MQ=255)
                                                  cacaTGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATa                                        >  1:3141448/1‑43 (MQ=255)
                                                  cacaTGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATa                                        >  1:1727668/1‑43 (MQ=255)
                                                  cacaTGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATa                                        >  1:1599933/1‑43 (MQ=255)
                                                  cacaTGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATa                                        >  1:2922288/1‑43 (MQ=255)
                                                  cacaTGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATa                                        <  1:22288/43‑1 (MQ=255)
                                                  cacaTGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATa                                        >  1:1432281/1‑43 (MQ=255)
                                                  cacaTGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGCGTTTGc               >  1:1456877/1‑68 (MQ=255)
                                                  cacaTGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGCGTTTGc               >  1:2805421/1‑68 (MQ=255)
                                                  cacaTGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGCGTTTGc               >  1:804258/1‑68 (MQ=255)
                                                  cacaTGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGCGTTTGc               >  1:669029/1‑68 (MQ=255)
                                                  cacaTGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGCGTTTGc               >  1:2442671/1‑68 (MQ=255)
                                                  cacaTGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGCGTTTGc               >  1:1379705/1‑68 (MQ=255)
                                                   acaTGCCAGATAATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGCGtt                  <  1:3091508/64‑1 (MQ=255)
                                                             ttATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGCGTTTGCCGCAAAAGACGc   <  1:2622312/69‑1 (MQ=255)
                                                             ttATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGCGTTTGCCGCAAAAGACGc   <  1:2618127/69‑1 (MQ=255)
                                                             ttATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGCGTTTGCCGCAAAAGACGc   <  1:1332460/69‑1 (MQ=255)
                                                             ttATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGCGTTTGCCGCAAAAGACGc   <  1:2307206/69‑1 (MQ=255)
                                                             ttATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGCGTTTGCCGCAAAAGACGc   <  1:1900399/69‑1 (MQ=255)
                                                              tATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGCGTTTGCCGCAAAAGACGcc  >  1:2845611/1‑69 (MQ=255)
                                                              |                                                                    
CCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGCGTTTGCCGCAAAAGACGCC  >  minE/103060‑103190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: