| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,263,009 | A→G | 41.5% | I58I (ATT→ATC) | cutC ← | copper homeostasis protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,263,009 | 0 | A | G | 41.5% | 32.4 / 69.2 | 94 | I58I (ATT→ATC) | cutC | copper homeostasis protein |
| Reads supporting (aligned to +/- strand): ref base A (21/34); new base G (27/12); total (48/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.61e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
Only 100 of 101 total aligned reads displayed.
CCTCAAGAATGGCGGCAAACTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATGCACAGGGATCGTCACCCGCTGGCGCACGGATTTCAGTACACCCAACGACGGCGTTAA > minE/1262951‑1263077 | ccTCAAGAATGGCGGCAAACTCACCGTCGCTGTAGC‑AAAATCACCACCGCGTGGGCGAATTATCGGATg < 1:2358533/69‑1 (MQ=255) cTCAAGAATGGCGGCAAACTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATg < 1:3043824/69‑1 (MQ=255) cTCAAGAATGGCGGCAAACTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATg < 1:2473933/69‑1 (MQ=255) cTCAAGAATGGCGGCAAACTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATg < 1:746065/69‑1 (MQ=255) cTCAAGAATGGCGGCAAACTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATg < 1:1120823/69‑1 (MQ=255) cTCAAGAATGGCGGCAAACTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATg < 1:2528118/69‑1 (MQ=255) cTCAAGAATGGCGGCAAACTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATg < 1:3297298/69‑1 (MQ=255) cTCAAGAATGGCGGCAAACTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATg < 1:2826755/69‑1 (MQ=255) cTCAAGAATGGCGGCAAACTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATg < 1:3107011/69‑1 (MQ=255) cAAGAATGGCGGCAAACTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATg > 1:3196843/1‑67 (MQ=255) cAAGAATGGCGGCAAACTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATg > 1:1661394/1‑67 (MQ=255) aTGGCGGCAAACTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATGcac > 1:2954382/1‑65 (MQ=255) aTGGCGGCAAACTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATGCACAggg > 1:2397994/1‑69 (MQ=255) aTGGCGGCAAACTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATGCACAggg > 1:486537/1‑69 (MQ=255) aTGGCGGCAAACTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATGCACAggg > 1:2130393/1‑69 (MQ=255) aTGGCGGCAAACTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATGCACAggg > 1:264642/1‑69 (MQ=255) aTGGCGGCAAACTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATGCACAggg > 1:3063282/1‑69 (MQ=255) gcggcAAACTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATGCACAGGGATc > 1:146948/1‑69 (MQ=255) gcggcAAACTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATGCACAGGGATc > 1:1884725/1‑69 (MQ=255) ggcAAACTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGGATAATCGGATGCACTGGGATCGt < 1:1428726/69‑1 (MQ=255) ggcAAACTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGGATAATCGGATGCACTGGGATCGt < 1:2473248/69‑1 (MQ=255) ggcAAACTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGGATAATCGGATGCACTGGGATCGt < 1:3270037/69‑1 (MQ=255) ggcAAACTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGGATAATCGGATGCACTGGGATCGt < 1:2388614/69‑1 (MQ=255) ggcAAACTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGGATAATCGGATGCACTGGGATCGt < 1:1675369/69‑1 (MQ=255) ggcAAACTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGGATAATCGGATGCACTGGGATCGt < 1:1195172/69‑1 (MQ=255) ggcAAACTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGGATAATCGGATGCACTGGGATCGt < 1:1565699/69‑1 (MQ=255) ggcAAACTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGGATAATCGGATGCACTGGGATCGt < 1:3120631/69‑1 (MQ=255) ggcAAACTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGGATAATCGGATGCACTGGGATCGt < 1:1555962/69‑1 (MQ=255) ggcAAACTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGGATAATCGGATGCACTGGGATCGt < 1:3348459/69‑1 (MQ=255) ggcAAACTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGGATAATCGGATGCACTGGGATCGt < 1:3206347/69‑1 (MQ=255) acAAACTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGGATAATCGGATGCACTGGGATCGt < 1:3266171/67‑1 (MQ=255) cAAACTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATg < 1:3269009/55‑1 (MQ=255) cTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGa > 1:769692/1‑40 (MQ=255) cTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGa > 1:640303/1‑40 (MQ=255) cTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGa > 1:1657404/1‑40 (MQ=255) cTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGa > 1:3387292/1‑40 (MQ=255) cTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATGCACAGGGATCGTCACCCg > 1:316262/1‑69 (MQ=255) cTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATGCACAGGGATCGTCACCCg > 1:201631/1‑69 (MQ=255) cTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATGCACAGGGATCGTCACCCg > 1:2659133/1‑69 (MQ=255) cTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATGCACAGGGATCGTCACCCg > 1:3261893/1‑69 (MQ=255) cTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATGCACAGGGATCGTCACCCg > 1:428486/1‑69 (MQ=255) cACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATGCACAGGGATCGt > 1:857997/1‑61 (MQ=255) cACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATGCACAGGGATCGTCACCCGc > 1:3093077/1‑68 (MQ=255) cACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATGCACAGGGATCGTCACCCGCt < 1:2468957/69‑1 (MQ=255) cACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATGCACAGGGATCGTCACCCGCt > 1:1231177/1‑69 (MQ=255) cACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATGCACAGGGATCGTCACCCGCt > 1:1715144/1‑69 (MQ=255) cACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATGCACAGGGATCGTCACCCGCt > 1:2616729/1‑69 (MQ=255) cACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATGCACAGGGATCGTCACCCGCt > 1:3428501/1‑69 (MQ=255) tGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATGCACAGGGATCGTCACCCGCTGGCGCACg < 1:334498/68‑1 (MQ=255) tGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATGCACAGGGATCGTCACCCGCTGGCGCACg < 1:2767168/68‑1 (MQ=255) tGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATGCACAGGGATCGTCACCCGCTGGCGCACg < 1:2684187/68‑1 (MQ=255) tGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATGCACAGGGATCGTCACCCGCTGGCGCACg < 1:1316378/68‑1 (MQ=255) tGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATGCACAGGGATCGTCACCCGCTGGCGCACg < 1:2632542/68‑1 (MQ=255) tGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATGCACAGGGATCGTCACCCGCTGGCGCACg < 1:3079299/68‑1 (MQ=255) tGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATGCACAGGGATCGTCACCCGCTGGCGCACg < 1:3287914/68‑1 (MQ=255) tGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATGCACAGGGATCGTCACCCGCTGGCGCACg < 1:1924900/68‑1 (MQ=255) gTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATGCACAGGGATCGTCACCCGCTGGCGCACg < 1:1985351/67‑1 (MQ=255) gTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATGCACAGGGATCGTCACCCGCTGGCGCACg < 1:2318884/67‑1 (MQ=255) aaaaTCACCACCGCGTGGGCGAATTATCGGATGCACAGGGATCGTCACCCGCTGGCGCACGTATTTCAg < 1:617351/69‑1 (MQ=255) aaaaTCACCACCGCGTGGGCGAATTATCGGATGCACAGGGATCGTCACCCGCTGGCGCACGGATTTCAg < 1:2045688/69‑1 (MQ=255) aaaaTCACCACCGCGTGGGCGAATTATCGGATGCACAGGGATCGTCACCCGCTGGCGCACGGATTTCAg < 1:609495/69‑1 (MQ=255) aaaaTCACCACCGCGTGGGCGAATTATCGGATGCACAGGGATCGTCACCCGCTGGCGCACGGATTTCAg < 1:1742397/69‑1 (MQ=255) aaaaTCACCACCGCGTGGGCGAATTATCGGATGCACAGGGATCGTCACCCGCTGGCGCACGGATTTCAg < 1:1918423/69‑1 (MQ=255) aaaaTCACCACCGCCTGGGCGAATTATCGGATGCACAGGGATCGTCACCCGCTGGCGCACGGATTTCAg < 1:1098094/69‑1 (MQ=255) caccGCGTGGGCGAATTATCGGATGCACAGGGATCGTCACCCGCTGGCGCACGGATTTCAGTACAccc < 1:950027/68‑1 (MQ=255) caccGCGTGGGCGAATTATCGGATGCACAGGGATCGTCACCCGCTGGCGCACGGATTTCAGTACAccc < 1:1705933/68‑1 (MQ=255) caccGCGTGGGCGAATTATCGGATGCACAGGGATCGTCACCCGCTGGCGCACGGATTTCAGTACAccc < 1:1099401/68‑1 (MQ=255) caccGCGTGGGCGAATTATCGGATGCACAGGGATCGTCACCCGCTGGCGCACGGATTTCAGTACAccc < 1:2549490/68‑1 (MQ=255) caccGCGTGGGCGAATTATCGGATGCACAGGGATCGACACCCGCTGGCGCACGGATTTCAGTACAccc < 1:1135016/68‑1 (MQ=255) gcgTGGGCGGATAATCGGATGCACTGGGATCGTCACCCGCTGGCGCACGGATTTCAGTACCCCCAacga > 1:300793/1‑69 (MQ=255) gcgTGGGCGGATAATCGGATGCACTGGGATCGTCACCCGCTGGCGCACGGATTTCAGTACCCCCAacga > 1:3377573/1‑69 (MQ=255) gcgTGGGCGGATAATCGGATGCACTGGGATCGTCACCCGCTGGCGCACGGATTTCAGTACCCCCAacga > 1:1557285/1‑69 (MQ=255) gcgTGGGCGGATAATCGGATGCACTGGGATCGTCACCCGCTGGCGCACGGATTTCAGTACCCCCAacga > 1:182610/1‑69 (MQ=255) gcgTGGGCGGATAATCGGATGCACTGGGATCGTCACCCGCTGGCGCACGGATTTCAGTACCCCCAacga > 1:2299263/1‑69 (MQ=255) gcgTGGGCGGATAATCGGATGCACTGGGATCGTCACCCGCTGGCGCACGGATTTCAGTACCCCCAacga > 1:700336/1‑69 (MQ=255) gcgTGGGCGGATAATCGGATGCACTGGGATCGTCACCCGCTGGCGCACGGATTTCAGTACCCCCAacga > 1:2535260/1‑69 (MQ=255) gcgTGGGCGGATAATCGGATGCACTGGGATCGTCACCCGCTGGCGCACGGATTTCAGTACCCCCAacga > 1:71049/1‑69 (MQ=255) gcgTGGGCGGATAATCGGATGCACTGGGATCGTCACCCGCTGGCGCACGGATTTCAGTACCCCCAacga > 1:2827386/1‑69 (MQ=255) cgTGGGCGGATAATCGGATGCACTGGGATCGTCACCCGCTGGCGCACGGATTTCCGTac > 1:2123630/1‑59 (MQ=255) cgTGGGCGGATAATCGGATGCACTGGGATCGTCACCCGCTGGCGCACGGATTTCAGTac > 1:1131182/1‑59 (MQ=255) cgTGGGCGGATAATCGGATGCACTGGGATCGTCACCCGCTGGCGCACGGATTTCAGTac > 1:1447723/1‑59 (MQ=255) cgTGGGCGGATAATCGGATGCACTGGGATCGTCACCCGCTGGCGCACGGATTTCAGTac > 1:359758/1‑59 (MQ=255) cgTGGGCGGATAATCGGATGCACTGGGATCGTCACCCGCTGGCGCACGGATTTCAGTac > 1:1523692/1‑59 (MQ=255) cgTGGGCGGATAATCGGATGCACTGGGATCGTCACCCGCTGGCGCACGGATTTCAGTac > 1:1024513/1‑59 (MQ=255) cgTGGGCGGATAATCGGATGCACTGGGATCGTCACCCGCTGGCGCACGGATTTCAGTac > 1:153691/1‑59 (MQ=255) cgTGGGCGGATAATCGGATGCACTGGGATCGTCACCCGCTGGCGCACGGATTTCAGTac > 1:101692/1‑59 (MQ=255) cgTGGGCGGATAATCGGATGCACTGGGATCGTCACCCGCTGGCGCACGGATTTCAGTac > 1:1538839/1‑59 (MQ=255) cgTGGGCGGATAATCGGATGCACTGGGATCGTCACCCGCTGGCGCACGGATTTCAGTac > 1:189637/1‑59 (MQ=255) cgTGGGCGGATAATCGGATGCACTGGGATCGTCACCCGCTGGCGCACGGATTTCAGTac > 1:2138905/1‑59 (MQ=255) cgTGGGCGGATAATCGGATGCACTGGGATCGTCACCCGCTGGCGCACGGATTTCAGTac > 1:3256305/1‑59 (MQ=255) cgTGGGCGGATAATCGGATGCACTGGGATCGTCACCCGCTGGCGCACGGATTTCAGTac > 1:2517021/1‑59 (MQ=255) cgTGGGCGGATAATCGGATGCACTGGGATCGTCACCCGCTGGCGCACGGATTTCAGTac > 1:3107792/1‑59 (MQ=255) cgTGGGCGGATAATCGGATGCACTGGGATCGTCACCCGCTGGCGCACGGATTTCAGTac > 1:2740363/1‑59 (MQ=255) cgTGGGCGGATAATCGGATGCACTGGGATCGTCACCCGCTGGCGCACGGATTTCAGTac > 1:2931817/1‑59 (MQ=255) cgTGGGCGGATAATCGGATGCACTGGGATCGTCACCCGCTGGCGCACGGATTTCAGTac > 1:2966206/1‑59 (MQ=255) cgTGGGCGGATAATCGGATGCACTGGGATCGTCACCCGCTGGCGCACGGATTTCAGTa > 1:1092417/1‑58 (MQ=255) ggCGAATTATCGGATGCACAGGGATCGTCACCCGCt < 1:1901463/36‑1 (MQ=255) aaTTATCGGATGCACAGGGATCGTCACCCGCTGGCGCACGGATTTCAGTACACCCAACGACGGCGTTaa < 1:1497717/69‑1 (MQ=255) aaTTATCGGATGCACAGGGATCGTCACCCGCTGGCGCACGGATTTCAGTACACCCAACGACGGCGTTaa < 1:1155394/69‑1 (MQ=255) aaTTATCGGATGCACAGGGATCGTCACCCGCTGGCGCACGGATTTCAGTACACCCAACGACGGCGTTaa < 1:2277666/69‑1 (MQ=255) | CCTCAAGAATGGCGGCAAACTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATGCACAGGGATCGTCACCCGCTGGCGCACGGATTTCAGTACACCCAACGACGGCGTTAA > minE/1262951‑1263077 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |