| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,266,786 | G→A | 45.6% | C207C (TGC→TGT) | yecA ← | conserved metal‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,266,786 | 0 | G | A | 45.6% | 6.1 / 34.4 | 46 | C207C (TGC→TGT) | yecA | conserved metal‑binding protein |
| Reads supporting (aligned to +/- strand): ref base G (19/6); new base A (13/8); total (32/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.49e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
GACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCC > minE/1266723‑1266846 | gACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAgg < 1:2827329/69‑1 (MQ=255) cTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGtt > 1:1043721/1‑53 (MQ=255) cTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGAt > 1:85748/1‑69 (MQ=255) tAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGt < 1:373346/68‑1 (MQ=255) tAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGt < 1:3052364/68‑1 (MQ=255) tAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGt < 1:1477379/68‑1 (MQ=255) tAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGt < 1:217249/68‑1 (MQ=255) tAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGt < 1:213190/68‑1 (MQ=255) ttCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTg > 1:2622521/1‑68 (MQ=255) ttCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTg > 1:812292/1‑68 (MQ=255) ttCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTg > 1:60339/1‑68 (MQ=255) ttCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTg > 1:3342923/1‑68 (MQ=255) ttCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTg > 1:2430014/1‑68 (MQ=255) ttCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTg > 1:1428097/1‑68 (MQ=255) tCTTACCACTACCGCACGGACAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGaa < 1:2078400/69‑1 (MQ=255) tCTTACCACTACCGCACGGACAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGaa < 1:1950647/69‑1 (MQ=255) tCTTACCACTACCGCACGGACAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGaa < 1:3381995/69‑1 (MQ=255) tCTTACCACTACCGCACGGACAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGaa < 1:146434/69‑1 (MQ=255) tCTTACCACTACCGCACGGACAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGaa < 1:2657612/69‑1 (MQ=255) tCTTACCACTACCGCACGGACAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGaa < 1:2659363/69‑1 (MQ=255) tCTTACCACTACCGCACGGACAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGaa < 1:2932500/69‑1 (MQ=255) tCTTACCACTACCGCACGGACAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGaa < 1:1435000/69‑1 (MQ=255) tACCACTACCGCACGGACAAGGATCGTTACGCCCCg > 1:585807/1‑36 (MQ=255) tACCACTACCGCACGGACAAGGATCGTTACGCCCCGGTTTctct > 1:2857294/1‑44 (MQ=255) tACCACTACCGCACGGACAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAg > 1:1084652/1‑69 (MQ=255) tACCACTACCGCACGGACAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAg > 1:788656/1‑69 (MQ=255) tACCACTACCGCACGGACAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAg > 1:120530/1‑69 (MQ=255) tACCACTACCGCACGGACAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAg > 1:1418349/1‑69 (MQ=255) tACCACTACCGCACGGACAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAg > 1:305623/1‑69 (MQ=255) tACCACTACCGCACGGACAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAg > 1:2712925/1‑69 (MQ=255) tACCACTACCGCACGGACAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAg > 1:2735903/1‑69 (MQ=255) tACCACTACCGCACGGACAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAg > 1:2590865/1‑69 (MQ=255) tACCACTACCGCACGGACAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAg > 1:1687286/1‑69 (MQ=255) tACCACTACCGCACGGACAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAg > 1:240443/1‑69 (MQ=255) tACCACTACCGCACGGACAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAg > 1:2317415/1‑69 (MQ=255) ccGCACGGGCAAGGATCGTTACGCCCCTGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTcc > 1:1433906/1‑69 (MQ=255) ccGCACGGGCAAGGATCGTTACGCCCCGTTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTcc > 1:1093105/1‑69 (MQ=255) ccGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTcc > 1:1989521/1‑69 (MQ=255) ccGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTcc > 1:746254/1‑69 (MQ=255) ccGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTcc > 1:726693/1‑69 (MQ=255) ccGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTcc > 1:2317736/1‑69 (MQ=255) ccGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTcc > 1:2385853/1‑69 (MQ=255) ccGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTcc > 1:572641/1‑69 (MQ=255) ccGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTcc > 1:434311/1‑69 (MQ=255) ccGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTcc > 1:296886/1‑69 (MQ=255) ccGCACGGGCAAGGATCGTTACGCCACGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTcc > 1:2614480/1‑69 (MQ=255) | GACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCC > minE/1266723‑1266846 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |