| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,269,009 | G→A | 100% | R148C (CGT→TGT) | uvrC ← | excinuclease UvrABC, endonuclease subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,269,009 | 0 | G | A | 75.1% | 19.1 / 2.9 | 20 | R148C (CGT→TGT) | uvrC | excinuclease UvrABC, endonuclease subunit |
| Reads supporting (aligned to +/- strand): ref base G (2/3); new base A (0/15); total (2/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.26e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCAACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTT > minE/1268951‑1269067 | cTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCAACGCCAAGTagc < 1:104908/68‑1 (MQ=255) gAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCAACGCCAAGTAGCAGCGGATGAttttt > 1:1854644/1‑69 (MQ=255) gAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCAACGCCAAGTAGCAGCGGATGAttttt > 1:1924473/1‑69 (MQ=255) ccTTTGGCAACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACa < 1:513536/66‑1 (MQ=255) ccTTTGGCAACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACAtt < 1:127129/68‑1 (MQ=255) ccTTTGGCAACACCAAGTAGCAGC‑‑ATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACAtttt < 1:197066/68‑1 (MQ=255) ttGGCAACATCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACAtttt < 1:1509872/67‑1 (MQ=255) ttGGCAACACCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACAtttt < 1:963550/67‑1 (MQ=255) ttGGCAACACCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACAtttt < 1:56829/67‑1 (MQ=255) ttGGCAACACCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACAtttt < 1:3434937/67‑1 (MQ=255) ttGGCAACACCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACAtttt < 1:3115116/67‑1 (MQ=255) ttGGCAACACCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACAtttt < 1:3031371/67‑1 (MQ=255) ttGGCAACACCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACAtttt < 1:3003103/67‑1 (MQ=255) ttGGCAACACCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACAtttt < 1:2465889/67‑1 (MQ=255) ttGGCAACACCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACAtttt < 1:2169559/67‑1 (MQ=255) ttGGCAACACCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACAtttt < 1:1952362/67‑1 (MQ=255) ttGGCAACACCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACAtttt < 1:1774932/67‑1 (MQ=255) ttGGCAACACCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACAtttt < 1:1661033/67‑1 (MQ=255) ttGGCAACACCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACAtttt < 1:1579774/67‑1 (MQ=255) ttGGCAACACCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACAtttt < 1:1059900/67‑1 (MQ=255) | CTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCAACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTT > minE/1268951‑1269067 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |