| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,271,105 | A→G | 28.6% | V384V (GTT→GTC) | yedQ ← | predicted diguanylate cyclase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,271,105 | 0 | A | G | 28.6% | 115.3 / 51.8 | 115 | V384V (GTT→GTC) | yedQ | predicted diguanylate cyclase |
| Reads supporting (aligned to +/- strand): ref base A (63/19); new base G (10/23); total (73/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.76e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01 | |||||||||||
Only 100 of 115 total aligned reads displayed.
TAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGCTAACCATCCGGCGAATCACATACCAGGAGATGAGTAACATGGTGGTAAAGAGCGCCCAC > minE/1271039‑1271162 | taAACGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGCTAAc > 1:944004/1‑69 (MQ=255)taAACGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGCTAAc > 1:1889794/1‑69 (MQ=255)taAACGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGCTAAc > 1:1372282/1‑69 (MQ=255)taAACGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGCTAAc > 1:771654/1‑69 (MQ=255)taAACGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGCTAAc > 1:854334/1‑69 (MQ=255) aaCGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGCTGACCa > 1:1104200/1‑69 (MQ=255) aaCGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGCTGACCa > 1:653030/1‑69 (MQ=255) aaCGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGCTGACCa > 1:1305773/1‑69 (MQ=255) aaCGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGCTGACCa > 1:2402432/1‑69 (MQ=255) aaCGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGCTGACCa > 1:408403/1‑69 (MQ=255) aaCGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGCTGACCa > 1:2423509/1‑69 (MQ=255) aaCGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGCTGACCa > 1:1722906/1‑69 (MQ=255) aaCGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGCTGACCa > 1:1547033/1‑69 (MQ=255) aaCGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGCTGACCa > 1:1632488/1‑69 (MQ=255) aaCGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGCTGACCa > 1:2735333/1‑69 (MQ=255) aaCGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGCTAACCa < 1:2883224/69‑1 (MQ=255) cgcgTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGCTAACCATc > 1:3184318/1‑69 (MQ=255) cgcgTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGCTAACCATc > 1:3126148/1‑69 (MQ=255) cgcgTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGCTAACCATc > 1:2735730/1‑69 (MQ=255) ttAAGGTGTCGTGCCACGCCTGTCACTGCAACGAGCTTTGCAGAACATACATGTTGCTAACCATCCgg > 1:1267838/1‑68 (MQ=255) tAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGCTAACCATCCGGCg < 1:2413024/69‑1 (MQ=255) aaGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGCTAACCATCCGGcta > 1:3144339/1‑67 (MQ=255) aaGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGCTAACCATCCGGCGa > 1:2699370/1‑69 (MQ=255) aaGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGCTAACCATCCGGCGa > 1:2817086/1‑69 (MQ=255) aaGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGCTAACCATCCGGCGa > 1:2642817/1‑69 (MQ=255) aaGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGCTAACCATCCGGCGa > 1:537440/1‑69 (MQ=255) aaGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGCTAACCATCCGGCGa > 1:1970629/1‑69 (MQ=255) aaGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGCTAACCATCCGGCGa > 1:107007/1‑69 (MQ=255) aaGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGCTAACCATCCGGCGa > 1:1342935/1‑69 (MQ=255) aaGGTGTCGTGCCACGACTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGCTAACCATCCGGCGa > 1:2592559/1‑69 (MQ=255) tgtCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGCTAACCATCCGGCGAATca < 1:1387870/69‑1 (MQ=255) tCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGCTAACCATCCGGCGAATcaca < 1:1854971/69‑1 (MQ=255) tCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGCTAACCATCCGGCGAATcaca < 1:156099/69‑1 (MQ=255) tCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGCTAACCATCCGGCGAATcaca < 1:1823407/69‑1 (MQ=255) gTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGCTAACCATCCGGCGAATcaca > 1:2285915/1‑67 (MQ=255) gTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGCTAACCATCCGGCGAATCACAt > 1:2759285/1‑68 (MQ=255) aCTGGAACGAGCTTTGCAGAACATACATGTTGCTGACCATCCGGCGAATCACATACCaggagaggag < 1:255303/67‑1 (MQ=255) aCTGCAACGAGCTTTGCAGAACATACATGTTGCTGACCATCCGGCGAATCACATACCaggagaggag < 1:425659/67‑1 (MQ=255) aCTGCAACGAGCTTTGCAGAACATACATGTTGCTGACCATCCGGCGAATCACATACCaggagaggag < 1:3171514/67‑1 (MQ=255) aCTGCAACGAGCTTTGCAGAACATACATGTTGCTGACCATCCGGCGAATCACATACCaggagaggag < 1:142782/67‑1 (MQ=255) aCTGCAACGAGCTTTGCAGAACATACATGTTGCTGACCATCCGGCGAATCACATACCaggagaggag < 1:1867210/67‑1 (MQ=255) aCTGCAACGAGCTTTGCAGAACATACATGTTGCTGACCATCCGGCGAATCACATACCaggagaggag < 1:582026/67‑1 (MQ=255) aCTGCAACGAGCTTTGCAGAACATACATGTTGCTGACCATCCGGCGAATCACATACCaggagaggag < 1:3401830/67‑1 (MQ=255) aCTGCAACGAGCTTTGCAGAACATACATGTTGCTGACCATCCGGCGAATCACATACCaggagaggag < 1:1526332/67‑1 (MQ=255) aCTGCAACGAGCTTTGCAGAACATACATGTTGCTGACCATCCGGCGAATCACATACCaggagaggag < 1:3435775/67‑1 (MQ=255) aCTGCAACGAGCTTTGCAGAACATACATGTTGCTGACCATCCGGCGAATCACATACCaggagaggag < 1:1681465/67‑1 (MQ=255) aCTGCAACGAGCTTTGCAGAACATACATGTTGCTGACCATCCGGCGAATCACATACCaggagaggag < 1:661271/67‑1 (MQ=255) aCTGCAACGAGCTTTGCAGAACATACATGTTGCTGACCATCCGGCGAATCACATACCaggagaggag < 1:739736/67‑1 (MQ=255) aCTGCAACGAGCTTTGCAGAACATACATGTTGCTGACCATCCGGCGAATCACATACCaggagaggag < 1:404006/67‑1 (MQ=255) aCTGCAACGAGCTTTGCAGAACATACATGTTGCTGACCATCCGGCGAATCACATACCaggagaggag < 1:2596701/67‑1 (MQ=255) aCTGCAACGAGCTTTGCAGAACATACATGTTGCTGACCATCCGGCGAATCACATACCaggagaggag < 1:2404894/67‑1 (MQ=255) aCTGCAACGAGCTTTGCAGAACATACATGTTGCTGACCATCCGGCGAATCACATACCaggagaggag < 1:1820502/67‑1 (MQ=255) aCTGCAACGAGCTTTGCAGAACATACATGTTGCTGACCATCCGGCGAATCACATACCaggagaggag < 1:2424454/67‑1 (MQ=255) aCTGCAACGAGCTTTGCAGAACATACATGTTGCTGACCATCCGGCGAATCACATACCaggagaggag < 1:1079283/67‑1 (MQ=255) aCTGCAACGAGCTTTGCAGAACATACATGTTGCTGACCATCCGGCGAATCACATACCaggagaggag < 1:2478865/67‑1 (MQ=255) aCTGCAACGAGCTTTGCAGAACATACATGTTGCTGACCATCCGGCGAATCACATACCaggagaggag < 1:2508756/67‑1 (MQ=255) aCTGCAACGAGCTTTGCAGAACATACATGTTGCTGACCATCCGGCGAATCACATACCaggagaggag < 1:1824080/67‑1 (MQ=255) gCAACGAGCTTTGCAGAACATACATGTTGCTAATCATCCGGCGAATCACATACCAGGAGATGAGTAACa > 1:3427024/1‑69 (MQ=255) gCAACGAGCTTTGCAGAACATACATGTTGCTAACCATCCGGCGAATCACATACCAGga < 1:1507802/58‑1 (MQ=255) gCAACGAGCTTTGCAGAACATACATGTTGCTAACCATCCGGCGAATCACATACCAGga < 1:849515/58‑1 (MQ=255) gCAACGAGCTTTGCAGAACATACATGTTGCTAACCATCCGGCGAATCACATACCAGGAGATGAGTAACa > 1:319243/1‑69 (MQ=255) gCAACGAGCTTTGCAGAACATACATGTTGCTAACCATCCGGCGAATCACATACCAGGAGATGAGTAACa > 1:1404849/1‑69 (MQ=255) gCAACGAGCTTTGCAGAACATACATGTTGCTAACCATCCGGCGAATCACATACCAGGAGATGAGTAACa > 1:350364/1‑69 (MQ=255) gCAACGAGCTTTGCAGAACATACATGTTGCTAACCATCCGGCGAATCACATACCAGGAGATGAGTAACa > 1:2216208/1‑69 (MQ=255) gCAACGAGCTTTGCAGAACATACATGTTGCTAACCATCCGGCGAATCACATACCAGGAGATGAGTAACa > 1:2259258/1‑69 (MQ=255) gCAACGAGCTTTGCAGAACATACATGTTGCTAACCATCCGGCGAATCACATACCAGGAGATGAGTAACa > 1:745354/1‑69 (MQ=255) gCAACGAGCTTTGCAGAACATACATGTTGCTAACCATCCGGCGAATCACATACCAGGAGATGAGTAACa > 1:3096920/1‑69 (MQ=255) gCAACGAGCTTTGCAGAACATACATGTTGCTAACCATCCGGCGAATCACATACCAGGAGATGAGTAACa > 1:3010803/1‑69 (MQ=255) gCAACGAGCTTTGCAGAACATACATGTTGCTAACCATCCGGCGAATCACATACCAGGAGATGAGTAACa > 1:2768340/1‑69 (MQ=255) gCAACGAGCTTTGCAGAACATACATGTTGCTAACCATCCGGCGAATCACATACCAGGAGATGAGTAACa > 1:2764492/1‑69 (MQ=255) gCAACGAGCTTTGCAGAACATACATGTTGCTAACCATCCGGCGAATCACATACCAGGAGATGAGTAACa > 1:1146338/1‑69 (MQ=255) gCAACGAGCTTTGCAGAACATACATGTTGCTAACCATCCGGCGAATCACATACCAGGAGATGAGTAACa > 1:2723162/1‑69 (MQ=255) gCAACGAGCTTTGCAGAACATACATGTTGCTAACCATCCGGCGAATCACATACCAGGAGATGAGTAACa > 1:627892/1‑69 (MQ=255) gCAACGAGCTTTGCAGAACATACATGTTGCTAACCATCCGGCGAATCACATACCAGGAGATGAGTAACa > 1:687808/1‑69 (MQ=255) gCAACGAGCTTTGCAGAACATACATGTTGCTAACCATCCGGCGAATCACATACCAGGAGATGAGTAACa > 1:2603883/1‑69 (MQ=255) gCAACGAGCTTTGCAGAACATACATGTTGCTAACCATCCGG‑GAATCACATACCAGGAGATGAGTAACa > 1:2633746/1‑68 (MQ=255) gCAACGAGCTTTGCAGAACATACATGTTGCTAACCATCCGG‑GAATCACATACCAGGAGATGAGTAACAt > 1:2207659/1‑69 (MQ=255) ttGCAGAACATACATGTTGCTAACCATCCGGCGAATCACATACCAGGAGATGAGTAACATGGTGGTa < 1:897840/67‑1 (MQ=255) cAGAACATACATGTTGCTAACCATCCGGCGAATCACATACCAGGAGATGAGTaa > 1:567267/1‑54 (MQ=255) cAGAACATACATGTTGCTAACCATCCGGCGAATCACATACCAGGAGATGAGTaa > 1:881010/1‑54 (MQ=255) cAGAACATACATGTTGCTAACCATCCGGCGAATCACATACCAGGAGATGAGTAACAAGGTGGTAAag < 1:1012131/67‑1 (MQ=255) cAGAACATACATGTTGCTAACCATCCGGCGAATCACATACCAGGACATGAGTaa > 1:2715607/1‑54 (MQ=255) acatacatGTTGCTAACCATCCGGCGAATCACATACCAGga < 1:1885339/41‑1 (MQ=255) acatacatGTTGCTAACCATCCGGCGAATCACATACCAGga < 1:318065/41‑1 (MQ=255) acatacatGTTGCTAACCATCCGGCGAATCACATACCAGga < 1:2997979/41‑1 (MQ=255) acatacatGTTGCTAACCATCCGGCGAATCACATACCAGga < 1:367869/41‑1 (MQ=255) acatacatGTTGCTAACCATCCGGCGAATCACATACCAGga < 1:1748335/41‑1 (MQ=255) acatacatGTTGCTAACCATCCGGCGAATCACATACCAGGAGATGAGTAACATGGTGGTAAAGAGCGcc < 1:1270566/69‑1 (MQ=255) acatacatGTTGCTAACCATCCGGCGAATCACATACCAGGAGATGAGTAACATGGTGGTAAAGAGCGcc < 1:2308697/69‑1 (MQ=255) acatacatGTTGCTAACCATCCGGCGAATCACATACCAGGAGATGAGTAACATGGTGGTAAAGAGCGcc < 1:778421/69‑1 (MQ=255) acatacatGTTGCTAACCATCCGGCGAATCACATACCAGGAGATGAGTAACATGGTGGTAAAGAGCGcc < 1:1757603/69‑1 (MQ=255) catacatGTTGCTAACCATCCGGCGAATCACATACCAGGAGATGAGTAACATGGTGGTAAAGAgcgc > 1:1981596/1‑67 (MQ=255) catacatGTTGCTAACCATCCGGCGAATCACATACCAGGAGATGAGTAACATGGTGGTAAAGAGCGcc > 1:711453/1‑68 (MQ=255) catacatGTTGCTAACCATCCGGCGAATCACATACCAGGAGATGAGTAACATGGTGGTAAAGAGCGcc > 1:193791/1‑68 (MQ=255) catacatGTTGCTAACCATCCGGCGAATCACATACCAGGAGATGAGTAACATGGTGGTAAAGAGCGcc > 1:2598609/1‑68 (MQ=255) catacatGTTGCTAACCATCCGGCGAATCACATACCAGGAGATGAGTAACATGGTGGTAAAGAGCGcc > 1:2688143/1‑68 (MQ=255) catacatGTTGCTAACCATCCGGCGAATCACATACCAGGAGATGAGTAACATGGTGGTAAAGAGCGcc > 1:3400854/1‑68 (MQ=255) catacatGTTGCTAACCATCCGGCGAATCACATACCAGGAGATGAGTAACATGATGGTAAAGAGCGcc > 1:2056348/1‑68 (MQ=255) catGTTGCTAACCATCCGGCGAATCACATACCAGGAGATGAGTAACATGGTGGTAAAGAGCGCCcac > 1:1409144/1‑67 (MQ=255) catGTTGCTAACCATCCGGCGAATCACATACCAGGAGATGAGTAACATGGTGGTAAAGAGCGCCca > 1:169667/1‑66 (MQ=255) | TAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGCTAACCATCCGGCGAATCACATACCAGGAGATGAGTAACATGGTGGTAAAGAGCGCCCAC > minE/1271039‑1271162 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |