| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 103,907 | G→A | 50.0% | P145P (CCG→CCA) | ampD → | N‑acetyl‑anhydromuranmyl‑L‑alanine amidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 103,907 | 0 | G | A | 50.0% | ‑2.3 / 72.9 | 80 | P145P (CCG→CCA) | ampD | N‑acetyl‑anhydromuranmyl‑L‑alanine amidase |
| Reads supporting (aligned to +/- strand): ref base G (16/24); new base A (17/23); total (33/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.07e-01 | |||||||||||
CTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGG > minE/103839‑103969 | cTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCg < 1:1073327/69‑1 (MQ=255)cTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCg < 1:3031815/69‑1 (MQ=255) tGCGCAGTATCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTaaaaa < 1:2084648/69‑1 (MQ=255) tGCGCAGTATCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTaaaaa < 1:2047764/69‑1 (MQ=255) cAGTATCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACAt < 1:2703846/68‑1 (MQ=255) cAGTATCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACAt < 1:3302241/68‑1 (MQ=255) cAGTATCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACAt < 1:3306261/68‑1 (MQ=255) cAGTATCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACAt < 1:1843084/68‑1 (MQ=255) cAGTATCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACAt < 1:254978/68‑1 (MQ=255) cAGTATCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACAt < 1:3066118/68‑1 (MQ=255) cAGTATCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACAt < 1:1750643/68‑1 (MQ=255) cAGTATCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACAt < 1:1366320/68‑1 (MQ=255) cAGTATCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACAt < 1:1086269/68‑1 (MQ=255) cAGTATCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACAt < 1:852018/68‑1 (MQ=255) cAGTATCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACAt < 1:799645/68‑1 (MQ=255) aTCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACgg > 1:3025380/1‑69 (MQ=255) tCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTaaa < 1:1502213/58‑1 (MQ=255) tCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTaaa < 1:2691550/58‑1 (MQ=255) tCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTaaa < 1:1841352/58‑1 (MQ=255) tCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTaaa < 1:3333844/58‑1 (MQ=255) tCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACgg > 1:659340/1‑68 (MQ=255) tCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACgg > 1:3051682/1‑68 (MQ=255) tCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACgg > 1:822743/1‑68 (MQ=255) tCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACgg > 1:1667056/1‑68 (MQ=255) tCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACgg > 1:2048434/1‑68 (MQ=255) tCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACgg > 1:2052387/1‑68 (MQ=255) tCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACgg > 1:2068336/1‑68 (MQ=255) cTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATtgt < 1:1566049/69‑1 (MQ=255) cTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATtgt < 1:447192/69‑1 (MQ=255) tGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGa < 1:2026663/69‑1 (MQ=255) gcggcgGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCAt > 1:2227987/1‑63 (MQ=255) gcggcgGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCAt > 1:1067478/1‑63 (MQ=255) tACGCGGGCACTGATTGATTGCTATCCGGATATCGCTaaaaa < 1:3069161/42‑1 (MQ=255) cgGGCACTGATTGATCGCTCTCCAGATATCGCTAATAACATGACGGGCCATTGTGATATTGCGCCGGa < 1:1537309/68‑1 (MQ=255) cgGGCACTGATTGATCGCTATCCAGATATCGCTAATAACATGACGGGCCATTGTGATATTGCGCCGGa < 1:3267144/68‑1 (MQ=255) cgGGCACTGATTGATCGCTATCCAGATATCGCTAATAACATGACGGGCCATTGTGATATTGCGCCGGa < 1:3428824/68‑1 (MQ=255) cgGGCACTGATTGATCGCTATCCAGATATCGCTAATAACATGACGGGCCATTGTGATATTGCGCCGGa < 1:3215060/68‑1 (MQ=255) cgGGCACTGATTGATCGCTATCCAGATATCGCTAATAACATGACGGGCCATTGTGATATTGCGCCGGa < 1:3211105/68‑1 (MQ=255) cgGGCACTGATTGATCGCTATCCAGATATCGCTAATAACATGACGGGCCATTGTGATATTGCGCCGGa < 1:396027/68‑1 (MQ=255) cgGGCACTGATTGATCGCTATCCAGATATCGCTAATAACATGACGGGCCATTGTGATATTGCGCCGGa < 1:412403/68‑1 (MQ=255) cgGGCACTGATTGATCGCTATCCAGATATCGCTAATAACATGACGGGCCATTGTGATATTGCGCCGGa < 1:432831/68‑1 (MQ=255) cgGGCACTGATTGATCGCTATCCAGATATCGCTAATAACATGACGGGCCATTGTGATATTGCGCCGGa < 1:712750/68‑1 (MQ=255) cgGGCACTGATTGATCGCTATCCAGATATCGCTAATAACATGACGGGCCATTGTGATATTGCGCCGGa < 1:2829391/68‑1 (MQ=255) cgGGCACTGATTGATCGCTATCCAGATATCGCTAATAACATGACGGGCCATTGTGATATTGCGCCGGa < 1:931658/68‑1 (MQ=255) cgGGCACTGATTGATCGCTATCCAGATATCGCTAATAACATGACGGGCCATTGTGATATTGCGCCGGa < 1:983483/68‑1 (MQ=255) cgGGCACTGATTGATCGCTATCCAGATATCGCTAATAACATGACGGGCCATTGTGATATTGCGCCGGa < 1:2756030/68‑1 (MQ=255) cgGGCACTGATTGATCGCTATCCAGATATCGCTAATAACATGACGGGCCATTGTGATATTGCGCCGGa < 1:254175/68‑1 (MQ=255) cgGGCACTGATTGATCGCTATCCAGATATCGCTAATAACATGACGGGCCATTGTGATATTGCGCCGGa < 1:1789697/68‑1 (MQ=255) cgGGCACTGATTGATCGCTATCCAGATATCGCTAATAACATGACGGGCCATTGTGATATTGCGCCGGa < 1:1991449/68‑1 (MQ=255) cgGGCACTGATTGATCGCTATCCAGATATCGCTAATAACATGACGGGCCATTGTGATATTGCGCCGGa < 1:207720/68‑1 (MQ=255) cgGGCACTGATTGATCGCTATCCAGATATCGCTAATAACATGACGGGCCATTGTGATATTGCGCCGGa < 1:244315/68‑1 (MQ=255) cgGGCACTGATTGATCGCTATCCAGATATCGCTAATAACATGACGGGCCATTGTGATATTGCGCCGGa < 1:1724932/68‑1 (MQ=255) cgGGCACTGATTGATCGCTATCCAGATATCGCTAATAACATGACGGGCCATTGTGATATTGCGCCGGa < 1:1218172/68‑1 (MQ=255) cgGGCACTGATTGATCGCTATCCAGATATCGCTAATAACATGACGGGCCATTGTGATATTGCGCCGGa < 1:2621155/68‑1 (MQ=255) cgGGCACTGATTGATCGCTATCCAGATATCGCTAATAACATGACGGGCCATTGTGATATTGCGCCGGa < 1:2656179/68‑1 (MQ=255) cgGGCACTGATTGATCGCTATCCAGATATCGCTAATAACATGACGGGCCATTGTGATATTGCGCCGGa < 1:2663030/68‑1 (MQ=255) aCTGATTGATCGCTATCCAGATATCGCTAATAACATGACGGTCCATTGTGATATTgcgc > 1:1163058/1‑59 (MQ=255) aCTGATTGATCGCTATCCAGATATCGCTAATAACATGACGGGCCATTGTGATATTgcgc > 1:1235776/1‑59 (MQ=255) aCTGATTGATCGCTATCCAGATATCGCTAATAACATGACGGGCCATTGTGATATTgcgc > 1:1838878/1‑59 (MQ=255) aCTGATTGATCGCTATCCAGATATCGCTAATAACATGACGGGCCATTGTGATATTgcgc > 1:772481/1‑59 (MQ=255) aCTGATTGATCGCTATCCAGATATCGCTAATAACATGACGGGCCATTGTGATATTgcgc > 1:1120121/1‑59 (MQ=255) aCTGATTGATCGCTATCCAGATATCGCTAATAACATGACGGGCCATTGTGATATTgcgc > 1:424635/1‑59 (MQ=255) aCTGATTGATCGCTATCCAGATATCGCTAATAACATGACGGGCCATTGTGATATTgcgc > 1:938444/1‑59 (MQ=255) aCTGATTGATCGCTATCCAGATATCGCTAATAACATGACGGGCCATTGTGATATTgcgc > 1:1783944/1‑59 (MQ=255) aCTGATTGATCGCTATCCAGATATCGCTAATAACATGACGGGCCATTGTGATATTgcgc > 1:1788154/1‑59 (MQ=255) aCTGATTGATCGCTATCCAGATATCGCTAATAACATGACGGGCCATTGTGATATTgcgc > 1:1024783/1‑59 (MQ=255) aCTGATTGATCGCTATCCAGATATCGCTAATAACATGACGGGCCATTGTGATATTgcgc > 1:3335886/1‑59 (MQ=255) aCTGATTGATCGCTATCCAGATATCGCTAATAACATGACGGGCCATTGTGATATTgcgc > 1:3115524/1‑59 (MQ=255) aCTGATTGATCGCTATCCAGATATCGCTAATAACATGACGGGCCATTGTGATATTgcgc > 1:2550487/1‑59 (MQ=255) aCTGATTGATCGCTATCCAGATATCGCTAATAACATGACGGGCCATTGTGATATTgcgc > 1:3009940/1‑59 (MQ=255) aCTGATTGATCGCTATCCAGATATCGCTAATAACATGACGGGCCATTGTGATATTgcgc > 1:2950320/1‑59 (MQ=255) aCTGATTGATCGCTATCCAGATATCGCTAATAACATGACGGGCCATTGTGATATTgc > 1:2804756/1‑57 (MQ=255) aCTGATTGATCGCTATCCAGATATCACTAATAACATGACGGGCCATTGTGATATTgcgc > 1:3005960/1‑59 (MQ=255) cTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGaaa > 1:309259/1‑69 (MQ=255) tATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCgg < 1:233810/68‑1 (MQ=255) tATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCgg < 1:86038/68‑1 (MQ=255) cGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGa > 1:2748529/1‑46 (MQ=255) cGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCg > 1:1924233/1‑57 (MQ=255) cGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCg > 1:1337200/1‑57 (MQ=255) cGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCg > 1:129690/1‑57 (MQ=255) cGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCg < 1:1282494/57‑1 (MQ=255) cGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCg > 1:2593106/1‑57 (MQ=255) | CTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGG > minE/103839‑103969 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |