| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,272,913 | C→T | 55.9% | A231A (GCG→GCA) | yedI ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,272,913 | 0 | C | T | 55.9% | 9.8 / 45.2 | 59 | A231A (GCG→GCA) | yedI | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (22/4); new base T (16/17); total (38/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.85e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
CCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCC > minE/1272854‑1272961 | ccATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTtcatc > 1:95731/1‑69 (MQ=255)ccATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTtcatc > 1:614345/1‑69 (MQ=255)ccATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTtcatc > 1:1202411/1‑69 (MQ=255)ccATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTtcatc > 1:192564/1‑69 (MQ=255)ccATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTtcatc > 1:3219503/1‑69 (MQ=255)ccATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTtcatc > 1:1623245/1‑69 (MQ=255)ccATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTtcatc > 1:1371583/1‑69 (MQ=255) tACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGc > 1:2074438/1‑67 (MQ=255) tACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCa > 1:1692622/1‑69 (MQ=255) tACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCa > 1:2810769/1‑69 (MQ=255) tACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCa > 1:1725226/1‑69 (MQ=255) tACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCa > 1:3218974/1‑69 (MQ=255) ccACAATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAgg > 1:1266129/1‑69 (MQ=255) ccgccgccgACGAGGAACATCGCCAGCGTGCCGACAATCGATAATGCTTTCATCAGCCAGGGCGCGATa < 1:2780936/66‑1 (MQ=255) ccgccgccgACGAGGAACATCGCCAGCGTGCCGACAATCGATAATGCTTTCATCAGCCAGGGCGCGATa < 1:228999/66‑1 (MQ=255) ccgccgccgACGAGGAACATCGCCAGCGTGCCGACAATCGATAATGCTTTCATCAGCCAGGGCGCGATa < 1:2135441/66‑1 (MQ=255) ccgccgccgACGAGGAACATCGCCAGCGTGCCGACAATCGATAATGCTTTCATCAGCCAGGGCGCGATa < 1:237691/66‑1 (MQ=255) ccgccgccgACGAGGAACATCGCCAGCGTGCCGACAATCGATAATGCTTTCATCAGCCAGGGCGCGATa < 1:532120/66‑1 (MQ=255) ccgccgccgACGAGGAACATCGCCAGCGTGCCGACAATCGATAATGCTTTCATCAGCCAGGGCGCGATa < 1:2463364/66‑1 (MQ=255) ccgccgccgACGAGGAACATCGCCAGCGTGCCGACAATCGATAATGCTTTCATCAGCCAGGGCGCGATa < 1:3265166/66‑1 (MQ=255) ccgccgccgACGAGGAACATCGCCAGCGTGCCGACAATCGATAATGCTTTCATCAGCCAGGGCGCGATa < 1:265508/66‑1 (MQ=255) ccgccgccgACGAGGAACATCGCCAGCGTGCCGACAATCGATAATGCTTTCATCAGCCAGGGCGCGATa < 1:1763100/66‑1 (MQ=255) ccgccgccgACGAGGAACATCGCCAGCGTGCCGACAATCGATAATGCTTTCATCAGCCAGGGCGCGATa < 1:3060486/66‑1 (MQ=255) ccgccgccgACGAGGAACATCGCCAGCGTGCCGACAATCGATAATGCTTTCATCAGCCAGGGCGCGATa < 1:2841113/66‑1 (MQ=255) gccgccGAGGAACATCGCCAGCGTGCCGACAATCGATAATGCTTTCATCAGCCAGGGCGCGATAATCa > 1:2296519/1‑68 (MQ=255) gccgACGAGGAACATCGCCAGCGTGCCGACAATCGATAATGCTTtcatc > 1:2292638/1‑49 (MQ=255) gccgACGAGGAACATCGCCAGCGTGCCGACAATCGATAATGCTTTCATCAGCCAGGGCGCGATAATc > 1:2999945/1‑67 (MQ=255) gccgACGAGGAACATCGCCAGCGTGCCGACAATCGATAATGCTTTCATCAGCCAGGGCGCGATAATCa > 1:2814599/1‑68 (MQ=255) gccgACGAGGAACATCGCCAGCGTGCCGACAATCGATAATGCTTTCATCAGCCAGGGCGCGATAATCa > 1:1025355/1‑68 (MQ=255) gccgACGAGGAACATCGCCAGCGTGCCGACAATCGATAATGCTTTCATCAGCCAGGGCGCGATAATCa > 1:2911899/1‑68 (MQ=255) gccgACGAGGAACATCGCCAGCGTGCCGACAATCGATAATGCTTTCATCAGCCAGGGCGCGATAATCa > 1:2148182/1‑68 (MQ=255) gccgACGAGGAACATCGCCAGCGTGCCGACAATCGATAATGCTTTCATCAGCCAGGGCGCGATAATCa > 1:1244268/1‑68 (MQ=255) gccgACGAGGAACATCGCCAGCGTGCCGACAATCGATAATGCTTTCATCAGCCAGGGCGCGATAATCa > 1:1425847/1‑68 (MQ=255) gccgACGAGGAACATCGCCAGCGTGCCGACAATCGATAATGCTTTCATCAGCCAGGGCGCGATAATCa > 1:1120757/1‑68 (MQ=255) gccgACGAGGAACATCGCCAGCGTGCCGACAATCGATAATGCTTTCATCAGCCAGGGCGCGATAATCa > 1:1769374/1‑68 (MQ=255) gccgACGAGGAACATCGCCAGCGTGCCGACAATCGATAATGCTTTCATCAGCCAGGGCGCGATAATCa > 1:1798676/1‑68 (MQ=255) gccgACGAGGAACATCGCCAGCGTGCCGACAATCGATAATGCTTTCATCAGCCAGGGCGCGATAATCa > 1:1834991/1‑68 (MQ=255) gccgACGAGGAACATCGCCAGCGTGCCGACAATCGATAATGCTTTCATCAGCCAGGGCGCGATAATCa > 1:2165706/1‑68 (MQ=255) gccgACGAGGAACATCGCCAGCGTGCCGACAATCGATAATGCTTTCATCAGCCAGGGCGCGATAATCa > 1:2078602/1‑68 (MQ=255) gccgACGAGGAACATCGCCAGCGTGCCGACAATCGATAATGCTTTCATCAGCCAGGGCGCGATAATCa > 1:2110692/1‑68 (MQ=255) cgaGGAACATCGCCAGCGTGCCGACAATCGATAATGCTTTCATCAGCCAGGGCGCGAtt < 1:2510461/59‑2 (MQ=255) ggAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCtt < 1:547639/69‑1 (MQ=255) ggAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCtt < 1:1931361/69‑1 (MQ=255) ggAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCtt < 1:1349146/69‑1 (MQ=255) ggAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCtt < 1:1310326/69‑1 (MQ=255) aTCGCCAGCGTGCCGACAATCGATAATGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCt < 1:1000321/69‑1 (MQ=255) aTCGCCAGCGTGCCGACAATCGATAATGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCt < 1:1205291/69‑1 (MQ=255) aTCGCCAGCGTGCCGACAATCGATAATGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCt < 1:997493/69‑1 (MQ=255) aTCGCCAGCGTGCCGACAATCGATAATGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCt < 1:1322725/69‑1 (MQ=255) aTCGCCAGCGTGCCGACAATCGATAATGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCt < 1:250159/69‑1 (MQ=255) cAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGc > 1:3097249/1‑69 (MQ=255) cAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGc > 1:453591/1‑69 (MQ=255) cAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGc > 1:1454053/1‑69 (MQ=255) cAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGc > 1:2680443/1‑69 (MQ=255) cAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGc > 1:593242/1‑69 (MQ=255) cAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGc > 1:1049927/1‑69 (MQ=255) cAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGc > 1:1862627/1‑69 (MQ=255) aGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGcc > 1:2967056/1‑69 (MQ=255) aGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGcc > 1:3274635/1‑69 (MQ=255) | CCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCC > minE/1272854‑1272961 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |