| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,274,991 | A→G | 31.3% | Q101R (CAA→CGA) | yodA → | conserved metal‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,274,991 | 0 | A | G | 31.3% | 103.1 / 60.0 | 118 | Q101R (CAA→CGA) | yodA | conserved metal‑binding protein |
| Reads supporting (aligned to +/- strand): ref base A (34/47); new base G (19/18); total (53/65) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
Only 100 of 118 total aligned reads displayed.
TGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAA > minE/1274934‑1275055 | tGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCga < 1:828405/69‑1 (MQ=255)tGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCga < 1:3188831/69‑1 (MQ=255)tGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCga < 1:2651616/69‑1 (MQ=255)tGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCga < 1:3216348/69‑1 (MQ=255)tGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCga < 1:255025/69‑1 (MQ=255)tGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCga < 1:1945292/69‑1 (MQ=255)tGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCga < 1:1985674/69‑1 (MQ=255)tGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCga < 1:2487247/69‑1 (MQ=255)tGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCga < 1:1200910/69‑1 (MQ=255)tGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCga < 1:24220/69‑1 (MQ=255)tGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCga < 1:647612/69‑1 (MQ=255)tGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCga < 1:2063462/69‑1 (MQ=255)tGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCga < 1:823132/69‑1 (MQ=255) gATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAgatg < 1:1634266/69‑1 (MQ=255) gATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAgatg < 1:2411671/69‑1 (MQ=255) gATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAgatg < 1:1483772/69‑1 (MQ=255) gATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAgatg < 1:1451698/69‑1 (MQ=255) gATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAgatg < 1:3094676/69‑1 (MQ=255) gATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAgatg < 1:1405973/69‑1 (MQ=255) gATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAgatg < 1:1239836/69‑1 (MQ=255) gATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAgatg < 1:439460/69‑1 (MQ=255) gATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAgatg < 1:7594/69‑1 (MQ=255) gATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAgatg < 1:78347/69‑1 (MQ=255) gATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAgatg < 1:1358915/69‑1 (MQ=255) gATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAgat < 1:2762984/68‑1 (MQ=255) gATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAgat < 1:1479297/68‑1 (MQ=255) taaaactaaaacATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAgat > 1:1524784/1‑66 (MQ=255) taaaactaaaacATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAgat > 1:2965257/1‑66 (MQ=255) taaaactaaaacATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAgat > 1:446633/1‑66 (MQ=255) taaaactaaaacATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAgat > 1:1180766/1‑66 (MQ=255) taaaactaaaacATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAgat > 1:2518486/1‑66 (MQ=255) taaaactaaaacATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAgat > 1:1978889/1‑66 (MQ=255) taaaactaaaacATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAgat > 1:524003/1‑66 (MQ=255) taaaaccaaaacATTTGCTGAAATTAAAGATTATTATCGCAAAGGTTATGCGACAGATATCga < 1:2235855/63‑1 (MQ=255) taaaaccaaaacATTTGCTGAAATTAAAGATTATTATCGCAAAGGTTATGCGACAGATATCga < 1:237822/63‑1 (MQ=255) taaaaccaaaacATTTGCTGAAATTAAAGATTATTATCGCAAAGGTTATGCGACAGATATCga < 1:23422/63‑1 (MQ=255) taaaaccaaaacATTTGCTGAAATTAAAGATTATTATCGCAAAGGTTATGCGACAGATATCga < 1:527716/63‑1 (MQ=255) taaaaccaaaacATTTGCTGAAATTAAAGATTATTATCGCAAAGGTTATGCGACAGATATCga < 1:2545389/63‑1 (MQ=255) taaaaccaaaacATTTGCTGAAATTAAAGATTATTATCGCAAAGGTTATGCGACAGATATCga < 1:3215114/63‑1 (MQ=255) taaaaccaaaacATTTGCTGAAATTAAAGATTATTATCGCAAAGGTTATGCGACAGATATCga < 1:2941932/63‑1 (MQ=255) taaaaccaaaacATTTGCTGAAATTAAAGATTATTATCGCAAAGGTTATGCGACAGATATCga < 1:1026094/63‑1 (MQ=255) taaaaccaaaacATTTGCTGAAATTAAAGATTATTATCGCAAAGGTTATGCGACAGATATCga < 1:3142383/63‑1 (MQ=255) taaaaccaaaacATTTGCTGAAATTAAAGATTATTATCGCAAAGGTTATGCGACAGATATCga < 1:311668/63‑1 (MQ=255) taaaaccaaaacATTTGCTGAAATTAAAGATTATTATCGCAAAGGTTATGCGACAGATATCga < 1:1028198/63‑1 (MQ=255) taaaaccaaaacATTTGCTGAAATTAAAGATTATTATCGCAAAGGTTATGCGACAGATATCga < 1:1244232/63‑1 (MQ=255) taaaaccaaaacATTTGCTGAAATTAAAGATTATTATCGCAAAGGTTATGCGACAGATATCga < 1:2010501/63‑1 (MQ=255) taaaaccaaaacATATGCTGAAATTAAAGATTATTATCGCAAAGGTTATGCGACAGATATCga < 1:1414598/63‑1 (MQ=255) aaaactaaaacATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAgatgat > 1:79171/1‑68 (MQ=255) aaaactaaaacATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAgatgat > 1:1253383/1‑68 (MQ=255) aaaactaaaacATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGATATGAtt > 1:3410071/1‑69 (MQ=255) aaaactaaaacATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGAtt > 1:110127/1‑69 (MQ=255) aaaactaaaacATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGAtt > 1:359513/1‑69 (MQ=255) actaaaacATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTgg < 1:1049709/68‑1 (MQ=255) actaaaacATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTgg < 1:3185841/68‑1 (MQ=255) actaaaacATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTgg < 1:1398409/68‑1 (MQ=255) actaaaacATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTgg < 1:1548962/68‑1 (MQ=255) actaaaacATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTgg < 1:300029/68‑1 (MQ=255) actaaaacATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGc > 1:2916760/1‑69 (MQ=255) aaacATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAgat > 1:2556237/1‑58 (MQ=255) aaacATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAgat > 1:2623321/1‑58 (MQ=255) aaacATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAgat > 1:217681/1‑58 (MQ=255) aaacATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAgat > 1:672958/1‑58 (MQ=255) aaacATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAgat > 1:3430050/1‑58 (MQ=255) gCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCgt < 1:2136443/47‑2 (MQ=255) gAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCAt < 1:1172975/68‑1 (MQ=255) gAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCAt < 1:2276892/68‑1 (MQ=255) gAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCAt < 1:2197176/68‑1 (MQ=255) gAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCAt < 1:2085407/68‑1 (MQ=255) gAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCAt < 1:2818990/68‑1 (MQ=255) gAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCAt < 1:1621422/68‑1 (MQ=255) aaaTTAAAGATTATTATCGCAAAGGTTATGCGACAGATATCGAAATGATTGGCATTGAGGACGGCAttg > 1:1010053/1‑69 (MQ=255) aaaTTAAAGATTATTATCGCAAAGGTTATGCGACAGATATCGAAATGATTGGCATTGAGGACGGCAttg > 1:1232529/1‑69 (MQ=255) aaaTTAAAGATTATTATCGCAAAGGTTATGCGACAGATATCGAAATGATTGGCATTGAGGACGGCAttg > 1:483285/1‑69 (MQ=255) aaaTTAAAGATTATTATCGCAAAGGTTATGCGACAGATATCGAAATGATTGGCATTGAGGACGGCAttg > 1:463005/1‑69 (MQ=255) aaaTTAAAGATTATTATCGCAAAGGTTATGCGACAGATATCGAAATGATTGGCATTGAGGACGGCAttg > 1:450169/1‑69 (MQ=255) aaaTTAAAGATTATTATCGCAAAGGTTATGCGACAGATATCGAAATGATTGGCATTGAGGACGGCAttg > 1:258529/1‑69 (MQ=255) aaaTTAAAGATTATTATCGCAAAGGTTATGCGACAGATATCGAAATGATTGGCATTGAGGACGGCAttg > 1:672489/1‑69 (MQ=255) aaaTTAAAGATTATTATCGCAAAGGTTATGCGACAGATATCGAAATGATTGGCATTGAGGACGGCAttg > 1:1908812/1‑69 (MQ=255) aaaTTAAAGATTATTATCGCAAAGGTTATGCGACAGATATCGAAATGATTGGCATTGAGGACGGCAttg > 1:2726853/1‑69 (MQ=255) aaaTTAAAGATTATTATCGCAAAGGTTATGCGACAGATATCGAAATGATTGGCATTGAGGACGGCAttg > 1:273841/1‑69 (MQ=255) aaaTTAAAGATTATTATCGCAAAGGTTATGCGACAGATATCGAAATGATTGGCATTGAGGACGGCAttg > 1:719921/1‑69 (MQ=255) aaaTTAAAGATTATTATCGCAAAGGTTATGCGACAGATATCGAAATGATTGGCATTGAGGACGGCAttg > 1:750818/1‑69 (MQ=255) aaaTTAAAGATTATTATCGCAAAGGTTATGCGACAGATATCGAAATGATTGGCATTGAGGACGGCAttg > 1:1291302/1‑69 (MQ=255) aaaTTAAAGATTATTATCGCAAAGGTTATGCGACAGATATCGAAATGATTGGCATTGAGGACGGCAttg > 1:1912695/1‑69 (MQ=255) aaaTTAAAGATTATTATCGCAAAGGTTATGCGACAGATATCGAAATGATTGGCATTGAGGACGGCAttg > 1:2004338/1‑69 (MQ=255) ttAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCAttgttg < 1:3025248/69‑1 (MQ=255) ttAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCAttgttg < 1:1682885/69‑1 (MQ=255) aaaGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGaa > 1:1370561/1‑69 (MQ=255) tattatCACAAAGGTTATGCAACAGATATCGTGATGATTGGCATTg < 1:1211390/46‑1 (MQ=255) tatCACAAAGGTTATGCAACAGATATCGAGATGATTgg < 1:2918431/38‑1 (MQ=255) acaAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAaata > 1:425358/1‑69 (MQ=255) acaAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAaata > 1:1560970/1‑69 (MQ=255) acaAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAaata > 1:942532/1‑69 (MQ=255) acaAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAaat < 1:431889/68‑1 (MQ=255) tATGCAACAGATATCGAGATGATTGGCATTGAGGACTGCATTGTTGAATTCCATAGAAATAATGAaac > 1:274975/1‑68 (MQ=255) tATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGaaa > 1:128384/1‑67 (MQ=255) tATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAaac > 1:575972/1‑68 (MQ=255) tATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAaac > 1:1422792/1‑68 (MQ=255) tATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAaac > 1:1467048/1‑68 (MQ=255) aTGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAaacaa > 1:530460/1‑69 (MQ=255) | TGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAA > minE/1274934‑1275055 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |