BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,279,208	C→G	35.5%	H329Q (CAC→CAG)	yeeJ →	adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,279,208	0	C	G	35.5%	22.0 / 22.0	31	H329Q (CAC→CAG)	yeeJ	adhesin
Reads supporting (aligned to +/- strand): ref base C (6/14); new base G (9/2); total (15/16)
Fisher's exact test for biased strand distribution p-value = 9.15e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

ATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAA  >  minE/1279144‑1279268

aTGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTg                                                          >  1:2498701/1‑69 (MQ=255)

aTGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTg                                                          >  1:1994122/1‑69 (MQ=255)

      cACGCCCTGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                                                    <  1:3195472/69‑1 (MQ=255)

      cACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                                                    <  1:3079171/69‑1 (MQ=255)

      cACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                                                    <  1:3178636/69‑1 (MQ=255)

      cACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                                                    <  1:1044041/69‑1 (MQ=255)

      cACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                                                    <  1:2309720/69‑1 (MQ=255)

      cACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                                                    <  1:434437/69‑1 (MQ=255)

      cACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                                                    <  1:740351/69‑1 (MQ=255)

      cACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                                                    <  1:1786963/69‑1 (MQ=255)

      cACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                                                    <  1:1656299/69‑1 (MQ=255)

      cACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                                                    <  1:1490463/69‑1 (MQ=255)

      cACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                                                    <  1:1408253/69‑1 (MQ=255)

      cACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCCTGGCGGTa                                                    <  1:2420305/69‑1 (MQ=255)

      cACGCCCGGCCAATGGCTGGGATGTAAGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                                                    <  1:3160401/69‑1 (MQ=255)

               ccAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCgg                                                      >  1:833783/1‑58 (MQ=255)

               ccAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCt                                           >  1:78877/1‑69 (MQ=255)

                             gTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGtattat                             >  1:2401024/1‑69 (MQ=255)

                             gTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGtatta                              >  1:2509561/1‑68 (MQ=255)

                                              tttACCTGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCg                         <  1:2665779/54‑1 (MQ=255)

                                                tACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTg           <  1:1130252/68‑1 (MQ=255)

                                                    tgCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCg                         <  1:1989603/49‑1 (MQ=255)

                                                         ggCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTaaa  >  1:3063262/1‑68 (MQ=255)

                                                         ggCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTaaa  >  1:3040986/1‑68 (MQ=255)

                                                         ggCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTaaa  >  1:3154212/1‑68 (MQ=255)

                                                         ggCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTaaa  >  1:3003956/1‑68 (MQ=255)

                                                         ggCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTaaa  >  1:2092435/1‑68 (MQ=255)

                                                         ggCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTaaa  >  1:50163/1‑68 (MQ=255)

                                                         ggCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTaaa  >  1:1980576/1‑68 (MQ=255)

                                                         ggCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTaaa  >  1:1811717/1‑68 (MQ=255)

                                                         ggCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTaaa  >  1:820397/1‑68 (MQ=255)

ATGAAGCACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAA  >  minE/1279144‑1279268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`