BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,279,216	G→C	39.3%	G332A (GGT→GCT) ‡	yeeJ →	adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,279,216	0	G	C	39.3%	13.4 / 23.3	28	G332A (GGT→GCT) ‡	yeeJ	adhesin
Reads supporting (aligned to +/- strand): ref base G (3/14); new base C (9/2); total (12/16)
Fisher's exact test for biased strand distribution p-value = 1.48e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAA  >  minE/1279150‑1279268

cACGCCCTGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                                                    <  1:3195472/69‑1 (MQ=255)

cACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                                                    <  1:3079171/69‑1 (MQ=255)

cACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                                                    <  1:3178636/69‑1 (MQ=255)

cACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                                                    <  1:1044041/69‑1 (MQ=255)

cACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                                                    <  1:2309720/69‑1 (MQ=255)

cACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                                                    <  1:434437/69‑1 (MQ=255)

cACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                                                    <  1:740351/69‑1 (MQ=255)

cACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                                                    <  1:1786963/69‑1 (MQ=255)

cACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                                                    <  1:1656299/69‑1 (MQ=255)

cACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                                                    <  1:1490463/69‑1 (MQ=255)

cACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                                                    <  1:1408253/69‑1 (MQ=255)

cACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCCTGGCGGTa                                                    <  1:2420305/69‑1 (MQ=255)

cACGCCCGGCCAATGGCTGGGATGTAAGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTa                                                    <  1:3160401/69‑1 (MQ=255)

         ccAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCgg                                                      >  1:833783/1‑58 (MQ=255)

         ccAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCt                                           >  1:78877/1‑69 (MQ=255)

                       gTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGtattat                             >  1:2401024/1‑69 (MQ=255)

                       gTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGtatta                              >  1:2509561/1‑68 (MQ=255)

                                        tttACCTGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCg                         <  1:2665779/54‑1 (MQ=255)

                                          tACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTg           <  1:1130252/68‑1 (MQ=255)

                                              tgCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCg                         <  1:1989603/49‑1 (MQ=255)

                                                   ggCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTaaa  >  1:3063262/1‑68 (MQ=255)

                                                   ggCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTaaa  >  1:3154212/1‑68 (MQ=255)

                                                   ggCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTaaa  >  1:3040986/1‑68 (MQ=255)

                                                   ggCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTaaa  >  1:3003956/1‑68 (MQ=255)

                                                   ggCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTaaa  >  1:2092435/1‑68 (MQ=255)

                                                   ggCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTaaa  >  1:50163/1‑68 (MQ=255)

                                                   ggCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTaaa  >  1:1980576/1‑68 (MQ=255)

                                                   ggCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTaaa  >  1:1811717/1‑68 (MQ=255)

                                                   ggCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTaaa  >  1:820397/1‑68 (MQ=255)

CACGCCCGGCCAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAA  >  minE/1279150‑1279268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`