| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,283,867 | C→T | 100% | A1882A (GCC→GCT) | yeeJ → | adhesin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,283,867 | 0 | C | T | 83.5% | 34.0 / 0.4 | 24 | A1882A (GCC→GCT) | yeeJ | adhesin |
| Reads supporting (aligned to +/- strand): ref base C (4/0); new base T (0/20); total (4/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.41e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ACAACTGGAGGCTATTGATGAAAAACTGACACTCACGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAATGGCACTCCAGTGGAGGGTCAGGTC > minE/1283801‑1283933 | acaacTGGAGGCTATTGATGAAAAACTGACACTCACGGCGTCCAGTCCGCTTATCGGTGTCTATGcccc > 1:1059451/1‑69 (MQ=255)acaacTGGAGGCTATTGATGAAAAACTGACACTCACGGCGTCCAGTCCGCTTATCGGTGTCTATGcccc > 1:956656/1‑69 (MQ=255)acaacTGGAGGCTATTGATGAAAAACTGACACTCACGGCGTCCAGTCCGCTTATCGGTGTCTATGcccc > 1:497613/1‑69 (MQ=255)acaacTGGAGGCTATTGATGAAAAACTGACACTCACGGCGTCCAGTCCGCTTATCGGTGTCTATGcccc > 1:2394109/1‑69 (MQ=255)acaacTGGAGGCTATTGATGAAAAACTGACACTCACGGCGTCCAGTCCGCTTATCGGTGTCTATGcccc > 1:1025128/1‑69 (MQ=255)acaacTGGAGGCTATTGATGAAAAACTGACACTCACGGCGTCCAGTCCGCTTATCGGTGTCTATGccc > 1:3060845/1‑68 (MQ=255) cTGACACTCACGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTGACGGCAAc > 1:78385/1‑68 (MQ=255) tCCAGTCCGCTTATCGGTGTCAATGCTCCTAATGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAAt < 1:3033954/69‑1 (MQ=255) tCCAGTCCGCTTATCGGTGTCAATGCTCCTAAAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAAt < 1:2687106/69‑1 (MQ=255) tCCAGTCCGCTTATCGGTGTCAATGCTCCTAAAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAAt < 1:543986/69‑1 (MQ=255) tCCAGTCCGCTTATCGGTGTCAATGCTCCTAAAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAAt < 1:1122416/69‑1 (MQ=255) tCCAGTCCGCTTATCGGTGTCAATGCTCCTAAAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAAt < 1:45325/69‑1 (MQ=255) tCCAGTCCGCTTATCGGTGTCAATGCTCCTAAAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAAt < 1:383116/69‑1 (MQ=255) tCCAGTCCGCTTATCGGTGTCAATGCTCCTAAAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAAt < 1:2937748/69‑1 (MQ=255) tCCAGTCCGCTTATCGGTGTCAATGCTCCTAAAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAAt < 1:2915612/69‑1 (MQ=255) tCCAGTCCGCTTATCGGTGTCAATGCTCCTAAAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAAt < 1:2871590/69‑1 (MQ=255) tCCAGTCCGCTTATCGGTGTCAATGCTCCTAAAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAAt < 1:2618463/69‑1 (MQ=255) tCCAGTCCGCTTATCGGTGTCAATGCTCCTAAAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAAt < 1:1825674/69‑1 (MQ=255) tCCAGTCCGCTTATCGGTGTCAATGCTCCTAAAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAAt < 1:129814/69‑1 (MQ=255) tCCAGTCCGCTTATCGGTGTCAATGCTCCTAAAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAAt < 1:1580979/69‑1 (MQ=255) tCCAGTCCGCTTATCGGTGTCAATGCTCCTAAAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAAt < 1:1667608/69‑1 (MQ=255) tCCAGTCCGCTTATCGGTGTCAATGCTCCTAAAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAAt < 1:1733708/69‑1 (MQ=255) tCCAGTCCGCTTATCGGTGTCAATGCTCCTAAAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAAt < 1:2567730/69‑1 (MQ=255) tCCAGTCCGCTTATCGGTGTCAATGCTCCTAAAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAAt < 1:1839301/69‑1 (MQ=255) tCCAGTCCGCTTATCGGTGTCAATGCTCCTAAAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAAt < 1:1916408/69‑1 (MQ=255) tCCAGTCCGCTTATCGGTGTCAATGCTCCTAAAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAAt < 1:2345439/69‑1 (MQ=255) tCCAGTCCGCTTATCGGTGTCAATGCTCCTAAAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAAt < 1:1144247/69‑1 (MQ=255) tATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAATGGCACTCCAGTGGAGggtcag > 1:1994599/1‑69 (MQ=255) tATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAATGGCACTCCAGTGGAGggtcag > 1:1766839/1‑69 (MQ=255) tATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAATGGCACTCCAGTGGAGggtcag > 1:419018/1‑69 (MQ=255) ccccTACAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAATGGCACt > 1:3255198/1‑50 (MQ=255) ccccTACAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAATGGCACTCCAGTGGAGggtcaggtc > 1:3016655/1‑68 (MQ=255) ccccTACAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAATGGCACTCCAGTGGAGggtcaggtc > 1:1161320/1‑68 (MQ=255) ccccTACAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAATGGCACTCCAGTGGAGggtcaggtc > 1:1516141/1‑68 (MQ=255) ccccTACAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAATGGCACTCCAGTGGAGggtcaggt > 1:2818802/1‑67 (MQ=255) | ACAACTGGAGGCTATTGATGAAAAACTGACACTCACGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAATGGCACTCCAGTGGAGGGTCAGGTC > minE/1283801‑1283933 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |