| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,285,590 | T→A | 33.0% | pseudogene (324/327 nt) pseudogene (1050/1053 nt) |
yeeL ← yeeL ← |
ECK1975:JW5325:b1979; hypothetical protein, C‑ter fragment ECK1975:JW1961+JW5325:b4497; hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,285,590 | 0 | T | A | 33.0% | 125.7 / 92.3 | 121 | pseudogene (324/327 nt) pseudogene (1050/1053 nt) | yeeL yeeL | ECK1975:JW5325:b1979; hypothetical protein, C‑ter fragment ECK1975:JW1961+JW5325:b4497; hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (61/20); new base A (16/24); total (77/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.49e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01 | |||||||||||
Only 100 of 123 total aligned reads displayed.
TTGATGACACCACCGTTCATAACAGATCGGCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACTTTTTAATGCCTTTGCGAATTCATCCAGACTTATATTGTCAATTTTTTGC > minE/1285534‑1285656 | ttGATGACACCACCGTTCATAACAGATCGGCATTACTATGCATAAGTACTGTCTCATATTCTCGCCaa > 1:3194387/1‑68 (MQ=255) gaCACCACCGTTCATAACAGATCGGCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAacac < 1:1005506/68‑1 (MQ=255) gaCACCACCGTTCATAACAGATCGGCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAacac < 1:2375522/68‑1 (MQ=255) gaCACCACCGTTCATAACAGATCGGCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAacac > 1:2430781/1‑68 (MQ=255) gaCACCACCGTTCATAACAGATCGGCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAacac < 1:2706173/68‑1 (MQ=255) gaCACCACCGTTCATAACAGATCGGCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAacac < 1:1981090/68‑1 (MQ=255) gaCACCACCGTTCATAACAGATCGGCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAacac > 1:1902164/1‑68 (MQ=255) gaCACCACCGTTCATAACAGATCGGCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAacac < 1:1504442/68‑1 (MQ=255) gaCACCACCGTTCATAACAGATCGGCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAacac < 1:1499557/68‑1 (MQ=255) gaCACCACCGTTCATAACAGATCGGCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACt > 1:3424215/1‑69 (MQ=255) gaCACCACCGTTCATAACAGATCGGCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACt > 1:2052393/1‑69 (MQ=255) gaCACCACCGTTCATAACAGATCGGCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACt > 1:2044575/1‑69 (MQ=255) gaCACCACCGTTCATAACAGATCGGCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACt > 1:3013479/1‑69 (MQ=255) gaCACCACCGTTCATAACAGATCGGCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACt > 1:1865610/1‑69 (MQ=255) gaCACCACCGTTCATAACAGATCGGCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACt > 1:3177385/1‑69 (MQ=255) gaCACCACCGTTCATAACAGATCGGCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACt > 1:175306/1‑69 (MQ=255) gaCACCACCGTTCATAACAGATCGGCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACt > 1:1751177/1‑69 (MQ=255) gaCACCACCGTTCATAACAGATCGGCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACt > 1:32118/1‑69 (MQ=255) gaCACCACCGTTCATAACAGATCGGCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACt > 1:3286599/1‑69 (MQ=255) gaCACCACCGTTCATAACAGATCGGCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACt > 1:3301558/1‑69 (MQ=255) gaCACCACCGTTCATAACAGATCGGCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACt > 1:434823/1‑69 (MQ=255) gaCACCACCGTTCATAACAGATCGGCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACt > 1:1111805/1‑69 (MQ=255) gaCACCACCGTTCATAACAGATCGGCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACt > 1:3311707/1‑69 (MQ=255) gaCACCACCGTTCATAACAGATCGGCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACt > 1:1353961/1‑69 (MQ=255) gaCACCACCGTTCATAACAGATCGGCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAAAACt > 1:1446229/1‑69 (MQ=255) accGTTCATAACAGATCGGCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACTTTTTaa > 1:3347332/1‑69 (MQ=255) accGTTCATAACAGATCGGCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACTTTTTaa > 1:2006617/1‑69 (MQ=255) accGTTCATAACAGATCGGCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACTTTTTaa > 1:1258344/1‑69 (MQ=255) accGTTCATAACAGATCGGCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACTTTTTaa > 1:1642281/1‑69 (MQ=255) aCAGATCGGCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACTTTTTAATGCCTTTGCg < 1:1436269/69‑1 (MQ=255) aCAGATCGGCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACTTTTTAATGCCTTTGCg < 1:2630388/69‑1 (MQ=255) aCAGATCGGCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACTTTTTAATGCCTTTGCg < 1:857851/69‑1 (MQ=255) aCAGATCGGCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACTTTTTAATGCCTTTGCg < 1:3129773/69‑1 (MQ=255) aCAGATCGGCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACTTTTTAATGCCTTTGCg < 1:2146553/69‑1 (MQ=255) aCAGATCGGCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACTTTTTAATGCCTTTGCg < 1:588502/69‑1 (MQ=255) aCAGATCGGCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACTTTTTAATGCCTTTGCg < 1:1952227/69‑1 (MQ=255) aCAGATCGGCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACTTTTTAATGCCTTTGCg < 1:434784/69‑1 (MQ=255) aCAGATCGGCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACTTTTTAATGCCTTTGCg < 1:1725135/69‑1 (MQ=255) aCAGATCGGCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACTTTTTAATGCCTTTGCg < 1:79537/69‑1 (MQ=255) ggCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACTTTTTAATGCCTTTGCGAATTCa > 1:1499118/1‑68 (MQ=255) ggCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACTTTTTAATGCCTTTGCGAATTCa > 1:2777931/1‑68 (MQ=255) ggCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACTTTTTAATGCCTTTGCGAATTCa > 1:1264106/1‑68 (MQ=255) ggCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACTTTTTAATGCCTTTGCGAATTCa > 1:1416886/1‑68 (MQ=255) tcTATGCTTAAGTACTGCCTCAAATTCTCGGCAAAACACttt < 1:553176/41‑1 (MQ=255) aCTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACTTTTTAATGCCTTTGCGAATTCATCCAGa > 1:2938223/1‑68 (MQ=255) aCTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACTTTTTAATGCCTTTGCGAATTCATCCAGa > 1:709047/1‑68 (MQ=255) aCTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACTTTTTAATGCCTTTGCGAATTCATCCAGa > 1:2690665/1‑68 (MQ=255) aCTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACTTTTTAATGCCTTTGCGAATTCATCCAGa > 1:2584175/1‑68 (MQ=255) aCTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACTTTTTAATGCCTTTGCGAATTCATCCAGa > 1:1006568/1‑68 (MQ=255) aCTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACTTTTTAATGCCTTTGCGAATTCATCCAGa > 1:1068544/1‑68 (MQ=255) aCTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACTTTTTAATGCCTTTGCGAATTCATCCAGa > 1:168573/1‑68 (MQ=255) aCTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACTTTTTAATGCCTTTGCGAATTCATCCAGa > 1:1817218/1‑68 (MQ=255) aCTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACTTTTTAATGCCTTTGCGAATTCANCCAg > 1:964914/1‑67 (MQ=255) aCTATGCATAAGTACTGCCTCAAATTCTCGCCAAAACACttt < 1:1636252/42‑1 (MQ=255) aCTATGCATAAGTACTGCCTCAAATTCTCGCCAAAACACttt < 1:232633/42‑1 (MQ=255) aCTATGCATAAGTACTGCCTCAAATTCTCGCCAAAACACttt < 1:941350/42‑1 (MQ=255) aCTATGCATAAGTACTGCCTCAAATTCTCGCCAAAACACttt < 1:939344/42‑1 (MQ=255) aCTATGCATAAGTACTGCCTCAAATTCTCGCCAAAACACttt < 1:866138/42‑1 (MQ=255) aCTATGCATAAGTACTGCCTCAAATTCTCGCCAAAACACttt < 1:1031957/42‑1 (MQ=255) aCTATGCATAAGTACTGCCTCAAATTCTCGCCAAAACACttt < 1:1035840/42‑1 (MQ=255) aCTATGCATAAGTACTGCCTCAAATTCTCGCCAAAACACttt < 1:571946/42‑1 (MQ=255) aCTATGCATAAGTACTGCCTCAAATTCTCGCCAAAACACttt < 1:446997/42‑1 (MQ=255) aCTATGCATAAGTACTGCCTCAAATTCTCGCCAAAACACttt < 1:1633257/42‑1 (MQ=255) aCTATGCATAAGTACTGCCTCAAATTCTCGCCAAAACACttt < 1:2429514/42‑1 (MQ=255) aCTATGCATAAGTACTGCCTCAAATTCTCGCCAAAACACttt < 1:319498/42‑1 (MQ=255) aCTATGCATAAGTACTGCCTCAAATTCTCGCCAAAACACttt < 1:1868329/42‑1 (MQ=255) aCTATGCATAAGTACTGCCTCAAATTCTCGCCAAAACACttt < 1:1991665/42‑1 (MQ=255) aCTATGCATAAGTACTGCCTCAAATTCTCGCCAAAACACttt < 1:2638957/42‑1 (MQ=255) aCTATGCATAAGTACTGCCTCAAATTCTCGCCAAAACACttt < 1:3004902/42‑1 (MQ=255) aCTATGCATAAGTACTGCCTCAAATTCTCGCCAAAACACttt < 1:1884489/42‑1 (MQ=255) cTATGCATAAGTACTGCCTCAAATTCTCGCCAAAACACttt < 1:3172507/41‑1 (MQ=255) cTATGCATAAGTACTGCCTCAAATTCTCGCCAAAACACttt < 1:1484284/41‑1 (MQ=255) aTGCATAAGTACTGTCTCATATTCTCGCCAAAACACTTTTTAATGCCTTTGCGAATTCATCCAGACTta < 1:2997255/69‑1 (MQ=255) gCATAAGTACTGTCTCATATTCTCGCCAAAACACTTTTTAATGCCTTTGCGAATTCATCCAGACTtata > 1:319551/1‑69 (MQ=255) gCATAAGTACTGTCTCATATTCTCGCCAAAACACTTTTTAATGCCTTTGCGAATTCATCCAGACTtata > 1:951312/1‑69 (MQ=255) gCATAAGTACTGTCTCATATTCTCGCCAAAACACTTTTTAATGCCTTTGCGAATTCATCCAGACTtata > 1:2221064/1‑69 (MQ=255) gCATAAGTACTGTCTCATATTCTCGCCAAAACACTTTTTAATGCCTTTGCGAATTCATCCAGACTtata > 1:3187560/1‑69 (MQ=255) gCATAAGTACTGTCTCATATTCTCGCCAAAACACTTTTTAATGCCTTTGCGAATTCATCCAGACTtata > 1:3042665/1‑69 (MQ=255) gCATAAGTACTGTCTCATATTCTCGCCAAAACACTTTTTAATGCCTTTGCGAATTCATCCAGACTtat > 1:530838/1‑68 (MQ=255) aGTACTGTCTCATATTCTCGCCAAAACACTTTTTAATGCCTTTGCGAATTCATCCAGACTTATATTGTc > 1:2259341/1‑69 (MQ=255) aGTACTGTCTCATATTCTCGCCAAAACACTTTTTAATGCCTTTGCGAATTCATCCAGACTTATATTGTc > 1:3377717/1‑69 (MQ=255) aGTACTGTCTCATATTCTCGCCAAAACACTTTTTAATGCCTTTGCGAATTCATCCAGACTTATATTGTc > 1:3381242/1‑69 (MQ=255) aGTACTGTCTCATATTCTCGCCAAAACACTTTTTAATGCCTTTGCGAATTCATCCAGACTTATATTGTc > 1:2157412/1‑69 (MQ=255) aGTACTGTCTCATATTCTCGCCAAAACACTTTTTAATGCCTTTGCGAATTCATCCAGACTTATATTGTc > 1:2031532/1‑69 (MQ=255) gcctcAAATTCTCGCCAAAACACTTTTTAATGCCTTTGCGAATTCATCCAGACTTATATTGTCAAtttt > 1:2160186/3‑69 (MQ=255) gcctcAAATTCTCGCCAAAACACTTTTTAATGCCTTTGCGAATTCATCCAGACTTATATTGTCAAtttt > 1:2629308/3‑69 (MQ=255) gcctcAAATTCTCGCCAAAACACTTTTTAATGCCTTTGCGAATTCATCCAGACTTATATTGTCAAtttt > 1:1021365/3‑69 (MQ=255) gcctcAAATTCTCGCCAAAACACTTTTTAATGCCTTTGCGAATTCATCCAGACTTATATTGTCAAtttt > 1:658980/3‑69 (MQ=255) gcctcAAATTCTCGCCAAAACACTTTTTAATGCCTTTGCGAATTCATCCAGACTTATATTGTCAAtttt > 1:2011214/3‑69 (MQ=255) gcctcAAATTCTCGCCAAAACACTTTTTAATGCCTTTGCGAATTCATCCAGACTTATATTGTCAAtttt > 1:106879/3‑69 (MQ=255) gcctcAAATTCTCGCCAAAACACTTTTTAATGCCTTTGCGAATTCATCCAGACTTATATTGTCAAtttt > 1:449717/3‑69 (MQ=255) gcctcAAATTCTCGCCAAAACACTTTTTAATGCCTTTGCGAATTCATCCAGACTTATATTGTCAAtttt > 1:1093493/3‑69 (MQ=255) gcctcAAATTCTCGCCAAAACACTTTTTAATGCCTTTGCGAATTCATCCAGACTTATATTGTCAAtttt > 1:117249/3‑69 (MQ=255) gcctcAAATTCTCGCCAAAACACTTTTTAATGCCTTTGCGAATTCATCCAGACTTATATTGTCAAtttt > 1:1386110/3‑69 (MQ=255) gcctcAAATTCTCGCCAAAACACTTTTTAATGCCTTTGCGAATTCATCCAGACTTATATTGTCAAtttt > 1:3224519/3‑69 (MQ=255) gcctcAAATTCTCGCCAAAACACTTTTTAATGCCTTTGCGAATTCATCCAGACTTATATTGTCAAtttt > 1:1741052/3‑69 (MQ=255) gcctcAAATTCTCGCCAAAACACTTTTTAATGCCTTTGCGAATTCATCAAGACTTATATTGTCAAtttt > 1:1228187/3‑69 (MQ=255) gcctcAAATTCTCGCCAAAACACTTTTTAATGCCTTTCCGAATTCATCCAGACTTATATTGTCAAtttt > 1:2299907/3‑69 (MQ=255) atatTCTCGCCAAAACACTTTTTAATGCCTTTGCGAATTCATCCAGACTTATATTGTCAATTTTTTGc > 1:2769845/1‑68 (MQ=255) atatTCTCGCCAAAACACTTTTTAATGCCTTTGCGAATTCATCCAGACTTATATTGTCAATTTTTTGc > 1:3172645/1‑68 (MQ=255) | TTGATGACACCACCGTTCATAACAGATCGGCATTACTATGCATAAGTACTGTCTCATATTCTCGCCAAAACACTTTTTAATGCCTTTGCGAATTCATCCAGACTTATATTGTCAATTTTTTGC > minE/1285534‑1285656 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |