| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,286,261 | A→T | 22.0% | pseudogene (379/1053 nt) pseudogene (379/705 nt) |
yeeL ← yeeL ← |
ECK1975:JW1961+JW5325:b4497; hypothetical protein ECK1975:JW1961:b1980; hypothetical protein, N‑ter fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,286,261 | 0 | A | T | 22.0% | 98.5 / 23.9 | 59 | pseudogene (379/1053 nt) pseudogene (379/705 nt) | yeeL yeeL | ECK1975:JW1961+JW5325:b4497; hypothetical protein ECK1975:JW1961:b1980; hypothetical protein, N‑ter fragment |
| Reads supporting (aligned to +/- strand): ref base A (16/30); new base T (2/11); total (18/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.07e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.87e-01 | |||||||||||
AATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTCAATGCAAAGATCCGGCGTACCGTA > minE/1286201‑1286318 | aaTTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGttt < 1:123934/69‑1 (MQ=255)aaTTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGttt < 1:3136665/69‑1 (MQ=255)aaTTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGttt < 1:2701481/69‑1 (MQ=255)aaTTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGttt < 1:1875762/69‑1 (MQ=255) ataCCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCtt > 1:692183/1‑68 (MQ=255) ataCCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCtt > 1:3210882/1‑68 (MQ=255) ataCCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCtt > 1:1341991/1‑68 (MQ=255) ataCCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCtt > 1:1470468/1‑68 (MQ=255) ataCCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCtt > 1:2983526/1‑68 (MQ=255) ataCCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCtt > 1:2194589/1‑68 (MQ=255) ataCCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCtt > 1:2079086/1‑68 (MQ=255) ccAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTa > 1:925975/1‑69 (MQ=255) ccAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTa > 1:1110041/1‑69 (MQ=255) ccAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTa > 1:687139/1‑69 (MQ=255) ccAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTa > 1:3071495/1‑69 (MQ=255) ccAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTa > 1:2765897/1‑69 (MQ=255) aacaacCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACg < 1:1469781/69‑1 (MQ=255) aacaacCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACg < 1:1125994/69‑1 (MQ=255) aacaacCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACg < 1:2312764/69‑1 (MQ=255) aacaacCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACg < 1:2273072/69‑1 (MQ=255) aacaacCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACg < 1:2125603/69‑1 (MQ=255) aacCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCAt > 1:76410/1‑69 (MQ=255) aacCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCAt > 1:2964739/1‑69 (MQ=255) acCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATc < 1:226118/69‑1 (MQ=255) acCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATc < 1:2296919/69‑1 (MQ=255) acCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATc < 1:1626712/69‑1 (MQ=255) acCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATc < 1:197511/69‑1 (MQ=255) acCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATc < 1:1202699/69‑1 (MQ=255) ccTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATc < 1:32376/68‑1 (MQ=255) ggaaaTTCGTTTTTGCTTTCAACTGACTGATAAATATCATCGTTTTCAGGTTCTTTTTACGCATcgcc > 1:554190/3‑68 (MQ=255) ggaaaTTCGTTTTTGCTTTCAACTGACTGATAAATATCATCGTTTTCAGGTTCTTTTTACGCATcgcc > 1:3170287/3‑68 (MQ=255) aaTTCGTTTTTGCTTTCAATTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTc < 1:69612/67‑1 (MQ=255) aaTTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTc < 1:2930279/67‑1 (MQ=255) aaTTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTc < 1:326362/67‑1 (MQ=255) aaTTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTc < 1:3198658/67‑1 (MQ=255) aaTTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTc < 1:3084311/67‑1 (MQ=255) aaTTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTc < 1:161872/67‑1 (MQ=255) aaTTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTc < 1:2720064/67‑1 (MQ=255) aaTTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTc < 1:2679243/67‑1 (MQ=255) aaTTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTc < 1:2105243/67‑1 (MQ=255) ttCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTCAAt > 1:691152/1‑68 (MQ=255) ttCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTCAAt > 1:2073533/1‑68 (MQ=255) ttCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTCAATg < 1:2349564/69‑1 (MQ=255) tGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTCAATGCAAAGAt < 1:1389554/68‑1 (MQ=255) tGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTCAATGCAAAGAt < 1:1192714/68‑1 (MQ=255) gCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATc < 1:77135/51‑1 (MQ=255) tttCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTc < 1:648643/54‑1 (MQ=255) tgaTAAATATCATCGTTTTCAGGTTCTTTTTACGCATCGCCTCTATACAAAGATCCGGCGTACCGTa < 1:32214/67‑1 (MQ=255) tgaTAAATATCATCGTTTTCAGGTTCTTTTTACGCATCGCCTCTATACAAAGATCCGGCGTACCGTa < 1:339392/67‑1 (MQ=255) tgaTAAATATCATCGTTTTCAGGTTCTTTTTACGCATCGCCTCTATACAAAGATCCGGCGTACCGTa < 1:3426393/67‑1 (MQ=255) tgaTAAATATCATCGTTTTCAGGTTCTTTTTACGCATCGCCTCTATACAAAGATCCGGCGTACCGTa < 1:1335133/67‑1 (MQ=255) tgaTAAATATCATCGTTTTCAGGTTCTTTTTACGCATCGCCTCTATACAAAGATCCGGCGTACCGTa < 1:562554/67‑1 (MQ=255) tgaTAAATATCATCGTTTTCAGGTTCTTTTTACGCATCGCCTCTATACAAAGATCCGGCGTACCGTa < 1:2969632/67‑1 (MQ=255) tgaTAAATATCATCGTTTTCAGGTTCTTTTTACGCATCGCCTCTATACAAAGATCCGGCGTACCGTa < 1:2964526/67‑1 (MQ=255) tgaTAAATATCATCGTTTTCAGGTTCTTTTTACGCATCGCCTCTATACAAAGATCCGGCGTACCGTa < 1:1805576/67‑1 (MQ=255) tgaTAAATATCATCGTTTTCAGGTTCTTTTTACGCATCGCCTCTATACAAAGATCCGGCGTACCGTa < 1:2641996/67‑1 (MQ=255) tgaTAAATATCATCGTTTTCAGGTTCTTTTTACGCATCGCCTCTATACAAAGATCCGGCGTACCGTa < 1:1879271/67‑1 (MQ=255) tgaTAAATATCATCGTTTTCAGGTTCTTTTTACGCATCGCCTCTATACAAAGATCCGGCGTACCGTa < 1:1837672/67‑1 (MQ=255) gaTAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTCAATGCAAAGa < 1:3348770/52‑1 (MQ=255) | AATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTCAATGCAAAGATCCGGCGTACCGTA > minE/1286201‑1286318 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |