BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,287,757	C→T	14.5%	I268I (ATC→ATT)	shiA →	shikimate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,287,757	0	C	T	14.5%	183.3 / 15.0	102	I268I (ATC→ATT)	shiA	shikimate transporter
Reads supporting (aligned to +/- strand): ref base C (69/18); new base T (7/8); total (76/26)
Fisher's exact test for biased strand distribution p-value = 2.02e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

Only 100 of 102 total aligned reads displayed.

ACGACATCCCGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGCCTTTGCACTTAATTATTCAACCCAGAATATGGGGCTACCGCGCGA  >  minE/1287691‑1287810

aCGACATCCCGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGt                                                     >  1:409177/1‑69 (MQ=255)

aCGACATCCCGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGt                                                     >  1:2943252/1‑69 (MQ=255)

aCGACATCCCGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGt                                                     >  1:2953656/1‑69 (MQ=255)

aCGACATCCCGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGt                                                     <  1:2848587/69‑1 (MQ=255)

aCGACATCCCGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGt                                                     >  1:2583190/1‑69 (MQ=255)

aCGACATCCCGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGt                                                     >  1:2373768/1‑69 (MQ=255)

aCGACATCCCGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGt                                                     <  1:2200706/69‑1 (MQ=255)

aCGACATCCCGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGt                                                     >  1:2124456/1‑69 (MQ=255)

aCGACATCCCGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGt                                                     >  1:567490/1‑69 (MQ=255)

aCGACATCCCGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGt                                                     >  1:1734952/1‑69 (MQ=255)

aCGACATCCCGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGt                                                     >  1:1675631/1‑69 (MQ=255)

aCGACATCCCGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGt                                                     <  1:1649520/69‑1 (MQ=255)

aCGACATCCCGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGt                                                     >  1:1552122/1‑69 (MQ=255)

aCGACATCCCGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGt                                                     >  1:975906/1‑69 (MQ=255)

aCGACATCCCGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGt                                                     <  1:1048401/69‑1 (MQ=255)

aCGACATCCCGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGt                                                     >  1:1093778/1‑69 (MQ=255)

aCGACATCCCGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGt                                                     >  1:1266961/1‑69 (MQ=255)

aCGACATCCCGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGt                                                     >  1:1280083/1‑69 (MQ=255)

       cccGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGcc                                              <  1:1968092/69‑1 (MQ=255)

       cccGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGcc                                              <  1:1011211/69‑1 (MQ=255)

       cccGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGcc                                              <  1:3080065/69‑1 (MQ=255)

       cccGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGcc                                              <  1:3095712/69‑1 (MQ=255)

       cccGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGcc                                              <  1:3160389/69‑1 (MQ=255)

       cccGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGcc                                              <  1:564238/69‑1 (MQ=255)

       cccGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGcc                                              <  1:1569216/69‑1 (MQ=255)

        ccGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGt                                                     >  1:1589053/1‑61 (MQ=255)

        ccGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGcc                                              <  1:921244/68‑1 (MQ=255)

        ccGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGcc                                              <  1:1944148/68‑1 (MQ=255)

        ccGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGCCt                                             >  1:130385/1‑69 (MQ=255)

        ccGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGCCt                                             >  1:1520498/1‑69 (MQ=255)

        ccGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGCCt                                             >  1:953645/1‑69 (MQ=255)

        ccGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGCCt                                             >  1:3052068/1‑69 (MQ=255)

        ccGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGCCt                                             >  1:2764276/1‑69 (MQ=255)

        ccGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGCCt                                             >  1:266058/1‑69 (MQ=255)

        ccGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGCCt                                             >  1:2657403/1‑69 (MQ=255)

        ccGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGCCt                                             >  1:1582824/1‑69 (MQ=255)

        ccGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGCCt                                             >  1:1623339/1‑69 (MQ=255)

        ccGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGCCt                                             >  1:837580/1‑69 (MQ=255)

        ccGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGCCt                                             >  1:2077835/1‑69 (MQ=255)

        ccGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGCCt                                             >  1:205185/1‑69 (MQ=255)

        ccGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGCCt                                             >  1:1707719/1‑69 (MQ=255)

        ccGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGCCt                                             >  1:1323604/1‑69 (MQ=255)

        ccGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGCCt                                             >  1:53956/1‑69 (MQ=255)

        ccGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGCCt                                             >  1:333139/1‑69 (MQ=255)

        ccGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGCCt                                             >  1:1897395/1‑69 (MQ=255)

        ccGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGCCt                                             >  1:1947461/1‑69 (MQ=255)

        ccGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGCCt                                             >  1:3223799/1‑69 (MQ=255)

        ccGGTGCGTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGcc                                              >  1:3305034/1‑68 (MQ=255)

            tGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGCCTTTGc                                         <  1:1773871/69‑1 (MQ=255)

             gCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTg                                                >  1:861515/1‑61 (MQ=255)

             gCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTg                                                >  1:2717853/1‑61 (MQ=255)

             gCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTg                                                >  1:727298/1‑61 (MQ=255)

             gCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTg                                                >  1:743834/1‑61 (MQ=255)

             gCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTg                                                >  1:31071/1‑61 (MQ=255)

             gCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTg                                                >  1:3079129/1‑61 (MQ=255)

             gCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTg                                                >  1:3050114/1‑61 (MQ=255)

             gCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTg                                                >  1:300348/1‑61 (MQ=255)

             gCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTg                                                >  1:574781/1‑61 (MQ=255)

             gCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTg                                                >  1:2952281/1‑61 (MQ=255)

             gCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTg                                                >  1:676849/1‑61 (MQ=255)

             gCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTg                                                >  1:2923293/1‑61 (MQ=255)

             gCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTg                                                >  1:2919905/1‑61 (MQ=255)

             gCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTg                                                >  1:1194297/1‑61 (MQ=255)

             gCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTg                                                >  1:2951651/1‑61 (MQ=255)

             gCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTg                                                >  1:212019/1‑61 (MQ=255)

              cTTTTCTGAAGATTATTGCACTACGACTGTGCGAGTTGCTGACGATGTACATTGTTACTGCCTTTGCa                                        >  1:1100525/1‑68 (MQ=255)

              cTTTTCTGAAGATTATTGCACTACGACTGTGCGAGTTGCTGACGATGTACATTGTTACTGCCTTTGCa                                        >  1:908876/1‑68 (MQ=255)

              cTTTTCTGAAGATTATTGCACTACGACTGTGCGAGTTGCTGACGATGTACATTGTTACTGCCTTTGCa                                        >  1:1345579/1‑68 (MQ=255)

              cTTTTCTGAAGATTATTGCACTACGACTGTGCGAGTTGCTGACGATGTACATTGTTACTGCCTTTGCa                                        >  1:1779681/1‑68 (MQ=255)

              cTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTg                                                >  1:671357/1‑60 (MQ=255)

                tttcTGAAGATTATTGCACTACGACTGTGCGAGTTGCTGACGATGTACATTGTTACTGCCTTTGCACtt                                     >  1:412176/4‑69 (MQ=255)

                        gATTATTGCACTACGACTGTGCGAGTTGCTGACGATGTACATTGTTACTGCCTTTGCACTTAattat                               <  1:737554/67‑1 (MQ=255)

                        gATTATTGCACTACGACTGTGCGAGTTGCTGACGATGTACATTGTTACTGCCTTTGCACTTAattat                               <  1:1519027/67‑1 (MQ=255)

                        gATTATTGCACTACGACTGTGCGAGTTGCTGACGATGTACATTGTTACTGCCTTTGCACTTAattat                               <  1:3402456/67‑1 (MQ=255)

                        gATTATTGCACTACGACTGTGCGAGTTGCTGACGATGTACATTGTTACTGCCTTTGCACTTAattat                               <  1:2795478/67‑1 (MQ=255)

                        gATTATTGCACTACGACTGTGCGAGTTGCTGACGATGTACATTGTTACTGCCTTTGCACTTAattat                               <  1:577707/67‑1 (MQ=255)

                        gATTATTGCACTACGACTGTGCGAGTTGCTGACGATGTACATTGTTACTGCCTTTGCACTTAattat                               <  1:424617/67‑1 (MQ=255)

                        gATTATTGCACTACGACTGTGCGAGTTGCTGACGATGTACATTGTTACTGCCTTTGCACTTAattat                               <  1:1709085/67‑1 (MQ=255)

                        gATTATTGCACTACGACTGTGCGAGTTGCTGACGATGTACATTGTTACTGCCTTTGCACTTAattat                               <  1:2458602/67‑1 (MQ=255)

                           tattGCGCTACGACTGTGCGAATTGCTGACGATGTACATCg                                                      >  1:2835793/1‑41 (MQ=255)

                           tattGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGCCTTTGCACTTAATTATTCaa                           >  1:3115415/1‑68 (MQ=255)

                           tattGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGCCTTTGCACTTAATTATTCaa                           >  1:132421/1‑68 (MQ=255)

                           tattGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGCCTTTGCACTTAATTATTCaa                           >  1:1490488/1‑68 (MQ=255)

                           tattGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGCCTTTGCACTTAATTATTCaa                           >  1:2890132/1‑68 (MQ=255)

                           tattGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGCCTTTGCACTTAATTATTCaa                           >  1:3002134/1‑68 (MQ=255)

                           tattGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGCCTTTGCACTTAATTATTCaa                           >  1:1804552/1‑68 (MQ=255)

                           tattGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGCCTTTGCACTTAATTATTCaa                           >  1:2179581/1‑68 (MQ=255)

                           tattGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGCCTTTGCACTTAATTATTCaa                           >  1:1835080/1‑68 (MQ=255)

                           tattGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGCCTTTGCACTTAATTATTCaa                           >  1:385875/1‑68 (MQ=255)

                           tattGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGCCTTTGCACTTAATTATTCaa                           >  1:1861026/1‑68 (MQ=255)

                           tattGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGCCTTTGCACTTAATTATTCaa                           >  1:1869973/1‑68 (MQ=255)

                           tattGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGCCTTTGCACTTAATTATTCaa                           >  1:1067760/1‑68 (MQ=255)

                           tattGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTACCTTTGCACTTAATTATTCaa                           >  1:878134/1‑68 (MQ=255)

                                                 ttGCTGACGATGTACATCGTTAGTGCCTTTGCACTTAATTATTCAACCCAGAATATGGGGCTACcgcg     >  1:2124430/1‑68 (MQ=255)

                                                 ttGCTGACGATGTACATCGTTACTGCCTTTGCACTTAATTATTCAACCCAGAATATGGGGCTACcgcg     >  1:1035654/1‑68 (MQ=255)

                                                   gCTGACGATGTACATCGTTACTGCCTTTGCACTTAATTATTCAACCCAGAATATGGGGCTACCGCGCGa  >  1:1735997/1‑69 (MQ=255)

                                                                 tCGTTACTGCCTTTGCACTTAATTATTCAACCCAGAata                  <  1:2841402/39‑1 (MQ=255)

                                                                 tCGTTACTGCCTTTGCACTTAATTATTCAACCCAGAata                  <  1:665836/39‑1 (MQ=255)

                                                                 tCGTTACTGCCTTTGCACTTAATTATTCAACCCAGAata                  <  1:2052305/39‑1 (MQ=255)

                                                                 tCGTTACTGCCTTTGCACTTAATTATTCAACCCAGAata                  <  1:3138604/39‑1 (MQ=255)

ACGACATCCCGGTGCTTTCCTGAAGATTATTGCGCTACGACTGTGCGAATTGCTGACGATGTACATCGTTACTGCCTTTGCACTTAATTATTCAACCCAGAATATGGGGCTACCGCGCGA  >  minE/1287691‑1287810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`