| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,290,390 | C→T | 37.2% | G74G (GGC→GGT) | yeeN → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,290,390 | 0 | C | T | 37.2% | 69.6 / 68.8 | 110 | G74G (GGC→GGT) | yeeN | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (26/43); new base T (21/20); total (47/63) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.82e-01 | |||||||||||
Only 100 of 110 total aligned reads displayed.
CGTGCAAAGCAGGCACAAGTTCCAAAGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGTTATGAAGGCTTTGGTCCTAATGGCTCAATG > minE/1290325‑1290447 | cGTGCAAAGCAGGCACAAGTTCCAAAGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGCGga < 1:722324/69‑1 (MQ=255)cGTGCAAAGCAGGCACAAGTTCCAAAGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGCGga < 1:1990854/69‑1 (MQ=255) cAGGCACAAGTTCCAAAGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGCGGAGATGAAACg < 1:3436209/69‑1 (MQ=255) cAGGCACAAGTTCCAAAGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGCGGAGATGAAACg < 1:2891444/69‑1 (MQ=255) cAGGCACAAGTTCCAAAGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGCGGAGATGAAACg < 1:2578023/69‑1 (MQ=255) cAGGCACAAGTTCCAAAGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGCGGAGATGAAACg < 1:1066938/69‑1 (MQ=255) cAGGCACAAGTTCCAAAGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGCGGAGATGAAACg < 1:2373412/69‑1 (MQ=255) cAGGCACAAGTTCCAAAGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGCGGAGATGAAACg < 1:841144/69‑1 (MQ=255) cAGGCACAAGTTCCAAAGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGCGGAGATGAAACg < 1:2092818/69‑1 (MQ=255) cAGGCACAAGTTCCAAAGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGCGGAGATGAAACg < 1:1937309/69‑1 (MQ=255) cAGGCACAAGTTCCAAAGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGCGGAGATGAAACg < 1:1898713/69‑1 (MQ=255) tgCACAAGTTCCAAAGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGTGGAGATGAAACGtt > 1:1039562/2‑69 (MQ=255) ggCACAAGTTCCAAAGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGTGGAGATGAAACGtt > 1:1765582/1‑69 (MQ=255) ggCACAAGTTCCAAAGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGTGGAGATGAAACGtt > 1:1358913/1‑69 (MQ=255) ggCACAAGTTCCAAAGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGTGGAGATGAAACGtt > 1:2358453/1‑69 (MQ=255) ggCACAAGTTCCAAAGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGTGGAGATGAAACGtt > 1:812994/1‑69 (MQ=255) ggCACAAGTTCCAAAGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGTGGAGATGAAACGtt > 1:2151763/1‑69 (MQ=255) ggCACAAGTTCCAAAGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGTGGAGATGAAACGtt > 1:1188097/1‑69 (MQ=255) ggCACAAGTTCCAAAGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGTGGAGATGAAACGtt > 1:1937871/1‑69 (MQ=255) ggCACAAGTTCCAAAGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGTGGAGATGAAACGtt > 1:1239282/1‑69 (MQ=255) ggCACAAGTTCCAAAGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGTGGAGATGAAACGtt > 1:1922675/1‑69 (MQ=255) ggCACAAGTTCCAAAGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGTGGAGATGAAACGtt > 1:2593636/1‑69 (MQ=255) ggCACAAGTTCCAAAGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGTGGAGATGAAACGtt > 1:189802/1‑69 (MQ=255) ggCACAAGTTCCAAAGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGTGGAGATGAAACGtt > 1:277081/1‑69 (MQ=255) ggCACAAGTTCCAAAGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGTGGAGATGAAACGtt > 1:1668516/1‑69 (MQ=255) ggCACAAGTTCCAAAGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGTGGAGATGAAACGtt > 1:1667707/1‑69 (MQ=255) ggCACAAGTTCCAAAGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGTGGAGATGAAACGtt > 1:1393352/1‑69 (MQ=255) ggCACAAGTTCCAAAGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGTGGAGATGAAACGtt > 1:1434187/1‑69 (MQ=255) ggCACAAGTTCCAAAGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGTGGAGATGAAACGtt > 1:1466020/1‑69 (MQ=255) ggCACAAGTTCCAAAGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGTGGAGATGAAACGtt > 1:3352620/1‑69 (MQ=255) ggCACAAGTTCCAAAGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGTGGAGATGAAACGtt > 1:3290707/1‑69 (MQ=255) ggCACAAGTTCCAAAGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGTGGAGATGAAACGtt > 1:1576377/1‑69 (MQ=255) ggCACAAGTTCCAAAGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGCGGAGATGAAACGtt < 1:340144/69‑1 (MQ=255) gCACAAGTTCCAAAGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGCGGAGATGAAACGtt < 1:373278/68‑1 (MQ=255) gCACAAGTTCCAAAGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGCGGAGATGAAACGtt < 1:1058159/68‑1 (MQ=255) gCACAAGTTCCAAAGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGCGGAGATGAAACGtt < 1:260931/68‑1 (MQ=255) gCACAAGTTCCAAAGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGCGGAGATGAAACGtt < 1:3062925/68‑1 (MQ=255) aaacACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGt < 1:1840990/66‑1 (MQ=255) aaGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGTGGAGATGAAACGTTCGTACAggg < 1:1871545/65‑1 (MQ=255) aaGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGTGGAGATGAAACGTTCGTACAggg < 1:1859383/65‑1 (MQ=255) aaGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGTGGAGATGAAACGTTCGTACAggg < 1:2031395/65‑1 (MQ=255) aaGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGTGGAGATGAAACGTTCGTACAggg < 1:3352842/65‑1 (MQ=255) aaGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGTGGAGATGAAACGTTCGTACAggg < 1:1045625/65‑1 (MQ=255) aaGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGt < 1:1374823/69‑1 (MQ=255) aaGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGt < 1:2593215/69‑1 (MQ=255) aaGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGt < 1:3279854/69‑1 (MQ=255) aaGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGt < 1:243755/69‑1 (MQ=255) aaGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGt < 1:3226902/69‑1 (MQ=255) aaGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGt < 1:3181142/69‑1 (MQ=255) aaGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGt < 1:1721782/69‑1 (MQ=255) aaGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGt < 1:186533/69‑1 (MQ=255) aGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGt < 1:1195186/68‑1 (MQ=255) aGCACGTTATTGATAAAGAAATTGATAAAGCCAAAGGCGGTGGAGATGAAACGTTCGTACAggg < 1:157588/64‑1 (MQ=255) gCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGtt > 1:2645067/1‑68 (MQ=255) aTTGATAAAGCAATTGATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGt > 1:3137568/1‑50 (MQ=255) aTTGATAAAGCAATTGATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGTTATGAAGGc > 1:1626617/1‑69 (MQ=255) aTTGATAAAGCAATTGATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGTTATGAAGGc > 1:1088587/1‑69 (MQ=255) aTTGATAAAGCAATTGATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGTTATGAAGGc > 1:442981/1‑69 (MQ=255) aTTGATAAAGCAATTGATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGTTATGAAGGc > 1:3095640/1‑69 (MQ=255) aTTGATAAAGCAATTGATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGTTATGAAGGc > 1:3051646/1‑69 (MQ=255) aTTGATAAAGCAATTGATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGTTATGAAGGc > 1:2513255/1‑69 (MQ=255) tAAAGCAATTGATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGTTATGAAGGCTTTg < 1:878058/68‑1 (MQ=255) tAAAGCAATTGATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGTTATGAAGGCTTTg < 1:1591634/68‑1 (MQ=255) aaaGCAATTGATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGTTATGAAGGCTTTGGt < 1:3186945/69‑1 (MQ=255) aaGCAATTGATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGTTATGAAGGCTTTGGt < 1:1388768/68‑1 (MQ=255) aaGCAATTGATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGTTATGAAGGCTTTGGt < 1:2009953/68‑1 (MQ=255) ggaaTTGATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGTTATGAAGGCTTTGGTCCt < 1:2586542/67‑1 (MQ=255) gCAATTGATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGTTATGAAGGCTTTGGTCCt < 1:1605034/69‑1 (MQ=255) cAATTGATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGTTATGAAGGCTTTGGTCCTa < 1:155493/69‑1 (MQ=255) cAATTGATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGTTATGAAGGCTTTGGTCCTa < 1:908904/69‑1 (MQ=255) cAATTGATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGTTATGAAGGCTTTGGTCCTa < 1:3267842/69‑1 (MQ=255) cAATTGATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGTTATGAAGGCTTTGGTCCTa < 1:141774/69‑1 (MQ=255) cAATTGATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGTTATGAAGGCTTTGGTCCTa < 1:1113084/69‑1 (MQ=255) aaTTGAT‑AAGCCAAAGGCGGTGGAGATGAAACGTTCGTACAGGGACGTTATGAAGGCTTTGGTCCTAAt < 1:2420134/69‑1 (MQ=255) aTTGATAAAGCCAAAGGCGGTGGAGATGAAACGTTCGTACAGGGACGTTATGAAGGCTTTGGTCCTAAt < 1:3227947/69‑1 (MQ=255) aTTGATAAAGCCAAAGGCGGTGGAGATGAAACGTTCGTACAGGGACGTTATGAAGGCTTTGGTCCTAAt < 1:1407275/69‑1 (MQ=255) aTTGATAAAGCCAAAGGCGGTGGAGATGAAACGTTCGTACAGGGACGTTATGAAGGCTTTGGTCCTAAt < 1:1011351/69‑1 (MQ=255) aTTGATAAAGCCAAAGGCGGTGGAGATGAAACGTTCGTACAGGGACGTTATGAAGGCTTTGGTCCTAAt < 1:3206027/69‑1 (MQ=255) aTTGATAAAGCCAAAGGCGGTGGAGATGAAACGTTCGTACAGGGACGTTATGAAGGCTTTGGTCCTAAt < 1:909737/69‑1 (MQ=255) aTTGATAAAGCCAAAGGCGGTGGAGATGAAACGTTCGTACAGGGACGTTATGAAGGCTTTGGTCCTAAt < 1:2612501/69‑1 (MQ=255) aTTGATAAAGCCAAAGGCGGTGGAGATGAAACGTTCGTACAGGGACGTTATGAAGGCTTTCGTCCTAAt < 1:3313324/69‑1 (MQ=255) aTTGATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGTTATGAAGGCTTTGGTCCTaa > 1:740675/1‑68 (MQ=255) aTTGATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGTTATGAAGGCTTTGGTCCTaa > 1:2326289/1‑68 (MQ=255) aTTGATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGTTATGAAGGCTTTGGTCCTaa > 1:81662/1‑68 (MQ=255) aTTGATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGTTATGAAGGCTTTGGTCCTAAt > 1:1689315/1‑69 (MQ=255) aTTGATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGTTATGAAGGCTTTGGTCCTAAt > 1:2752144/1‑69 (MQ=255) aTTGATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGTTATGAAGGCTTTGGTCCTAAt > 1:1296584/1‑69 (MQ=255) aTTGATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGTTATGAAGGCTTTGGTCCTAAt > 1:771280/1‑69 (MQ=255) aTTGATAAAGACAAAGGCGGTGGAGATGAAACGTTCGTACAGGGACGTTATGAAGGCTTTGGTCCTAAt < 1:2296673/69‑1 (MQ=255) ttGATAAAGCCAAAGGCGGTGGAGATGAAACGTTCGTACAGGGACGTTATGAAGGCTTTGGTCCTAAt < 1:2154290/68‑1 (MQ=255) ttGATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGTTATGAAGGCTTTGGTCCt < 1:2268628/65‑1 (MQ=255) gATAAAGCCAAAGGCGGTGGAGATGAAACGTTCGTACAggg < 1:2401105/41‑1 (MQ=255) gATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGTTATGAAGGCTTTGGTCCt > 1:2651123/1‑63 (MQ=255) gATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGTTATGAAGGCTTTGGTCCt > 1:2526131/1‑63 (MQ=255) gATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGTTATGAAGGCTTTGGTCCt > 1:2230240/1‑63 (MQ=255) gATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGTTATGAAGGCTTTGGTCCt > 1:971776/1‑63 (MQ=255) aaGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGTTATGAAGGCTTTGGTCCTAATGGCTc > 1:2225553/1‑67 (MQ=255) aaGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGTTATGAAGGCTTTGGTCCTAATGGCTCa > 1:2221647/1‑68 (MQ=255) gCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGTTATGAAGGCTTTGGTCCTAATGGCTCAATg > 1:1053791/1‑69 (MQ=255) ccAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGTTATGAAGGCTTTGGTCCTAATGGCTAAATg > 1:1971527/1‑68 (MQ=255) | CGTGCAAAGCAGGCACAAGTTCCAAAGCACGTTATTGATAAAGCAATTGATAAAGCCAAAGGCGGCGGAGATGAAACGTTCGTGCAGGGACGTTATGAAGGCTTTGGTCCTAATGGCTCAATG > minE/1290325‑1290447 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |