| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,290,892 | T→G | 52.5% | intergenic (+7/+446) | yeeN → / ← asnW | conserved hypothetical protein/tRNA‑Asn |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,290,892 | 0 | T | G | 52.5% | 16.9 / 88.5 | 82 | intergenic (+7/+446) | yeeN/asnW | conserved hypothetical protein/tRNA‑Asn |
| Reads supporting (aligned to +/- strand): ref base T (29/10); new base G (23/20); total (52/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.70e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01 | |||||||||||
CCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATA > minE/1290830‑1290957 | cccTTGAAGATGACGACGATGTACAAAAAGTTTATTATAACGTCGCAAATCTCTAATTATCTTTTaaa > 1:2115101/1‑68 (MQ=255)cccTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTaaa > 1:2080844/1‑68 (MQ=255)cccTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTaaa > 1:2971974/1‑68 (MQ=255)cccTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTaaa > 1:305271/1‑68 (MQ=255) gAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTGTATGTTAAAGAAATc > 1:149635/1‑69 (MQ=255) gAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATATGTTAAAGAAAt > 1:462056/1‑68 (MQ=255) gAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATATGTTAAAGAAATc > 1:2598754/1‑69 (MQ=255) gAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATATGTTAAAGAAATc > 1:2066341/1‑69 (MQ=255) gAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATATGTTAAAGAAATc > 1:2860206/1‑69 (MQ=255) gAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATATGTTAAAGAAATc > 1:3035151/1‑69 (MQ=255) gAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATATGTTAAAGAAATc > 1:1780357/1‑69 (MQ=255) gAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATATGTTAAAGAAATc > 1:1759815/1‑69 (MQ=255) gAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATATGTTAAAGAAATc > 1:3116037/1‑69 (MQ=255) gAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATATGTTAAAGAAATc > 1:3291405/1‑69 (MQ=255) gAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATATGTTAAAGAAATc > 1:280171/1‑69 (MQ=255) gAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATATGTTAAAGAAATc > 1:1666632/1‑69 (MQ=255) gAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATATGTTAAAGAAATc > 1:43457/1‑69 (MQ=255) gAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATATGTTAAAGAAATc > 1:1614436/1‑69 (MQ=255) gAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATATGTTAAAGAAATc > 1:1570795/1‑69 (MQ=255) gAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATATGTTAAAGAAATc > 1:459613/1‑69 (MQ=255) gAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATATGTTAAAGAAATc > 1:2235119/1‑69 (MQ=255) gAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATATGTTAAAGAAATc > 1:2245582/1‑69 (MQ=255) gAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATATGTTAAAGAAATc > 1:2301699/1‑69 (MQ=255) gAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATATGTTAAAGAAATc > 1:637531/1‑69 (MQ=255) gAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATATGTTAAAGAAATc > 1:231632/1‑69 (MQ=255) gAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATATGTTAAAGAAATc > 1:790272/1‑69 (MQ=255) gAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATATGTTAAAGAAATc > 1:2424155/1‑69 (MQ=255) aGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATATGTTAAAGAAATCt < 1:2508404/68‑1 (MQ=255) aGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATATGTTAAAGAAATCt < 1:2523603/68‑1 (MQ=255) aGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATATGTTAAAGAAATCt < 1:1063739/68‑1 (MQ=255) aGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATATGTTAAAGAAATCt < 1:2887970/68‑1 (MQ=255) aGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATATGTTAAAGAAATCt < 1:3324244/68‑1 (MQ=255) aGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATATGTTAAAGAAATCt < 1:451760/68‑1 (MQ=255) aGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATATGTTAAAGAAATCt < 1:560572/68‑1 (MQ=255) aGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATATGTTAAAGAAATCt < 1:568012/68‑1 (MQ=255) aGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATATGTTAAAGAAATCt < 1:603864/68‑1 (MQ=255) aGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATATGTTAAAGAAATCt < 1:749528/68‑1 (MQ=255) aGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATATGTTAAAGAAATCt < 1:823649/68‑1 (MQ=255) aGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATATGTTAAAGAAATCt < 1:869825/68‑1 (MQ=255) aGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATATGTTAAAGAAATCt < 1:921475/68‑1 (MQ=255) aGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATATGTTAAAGAAATCt < 1:1832628/68‑1 (MQ=255) aGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATATGTTAAAGAAATCt < 1:1368883/68‑1 (MQ=255) aGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATATGTTAAAGAAATCt < 1:1661736/68‑1 (MQ=255) aGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATATGTTAAAGAAATCt < 1:250755/68‑1 (MQ=255) aGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATATGTTAAAGAAATCt < 1:1673529/68‑1 (MQ=255) aGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATATGTTAAAGAAATCt < 1:1723232/68‑1 (MQ=255) aTGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTc > 1:1187627/1‑69 (MQ=255) aTGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTc > 1:748085/1‑69 (MQ=255) aTGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTc > 1:3419650/1‑69 (MQ=255) aTGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTc > 1:3267152/1‑69 (MQ=255) aTGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTc > 1:1706753/1‑69 (MQ=255) aTGACGACGATGTACAAAAAGTTTATCA‑AACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTc > 1:2831309/1‑68 (MQ=255) gacgacGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCtt > 1:817744/1‑69 (MQ=255) gacgacGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCtt > 1:1433271/1‑69 (MQ=255) gacgacGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCtt > 1:1935955/1‑69 (MQ=255) gacgacGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCtt < 1:1648275/69‑1 (MQ=255) gacgacGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCtt < 1:1026724/69‑1 (MQ=255) gacgacGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCtt > 1:1118492/1‑69 (MQ=255) acgacgATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCttt > 1:2865887/1‑69 (MQ=255) acgacgATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCttt > 1:1259278/1‑69 (MQ=255) cgacgaTGTACAATAAGTTTATCATAACGTCGCAAATCTCTAATTATATGTTAAAGAAATCt < 1:97565/62‑1 (MQ=255) gacgaTGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTAc > 1:90024/1‑69 (MQ=255) gacgaTGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTAc > 1:2016324/1‑69 (MQ=255) tACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCttt > 1:3192215/1‑43 (MQ=255) tACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGAt > 1:1207084/1‑69 (MQ=255) tACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGAt > 1:2695749/1‑69 (MQ=255) tATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAAtct > 1:1901302/1‑69 (MQ=255) aTAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCat < 1:3413348/68‑1 (MQ=255) aTAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCat < 1:1362381/68‑1 (MQ=255) aTAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCat < 1:1156497/68‑1 (MQ=255) aTAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCat < 1:2030714/68‑1 (MQ=255) aTAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCat < 1:2802953/68‑1 (MQ=255) aTAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCat < 1:2187033/68‑1 (MQ=255) aTAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCat < 1:2193275/68‑1 (MQ=255) tctAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATaaaa > 1:2605932/1‑68 (MQ=255) tATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAaatataa > 1:2691738/1‑69 (MQ=255) tATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAaatataa < 1:2499436/69‑1 (MQ=255) cTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAtaatataata > 1:906220/1‑68 (MQ=255) cTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAaatataata > 1:1485846/1‑68 (MQ=255) cTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAaatataata > 1:389625/1‑68 (MQ=255) cTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAaatataata > 1:349767/1‑68 (MQ=255) cTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAaatataata > 1:1075329/1‑68 (MQ=255) cTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAaatataat > 1:2758914/1‑67 (MQ=255) | CCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATA > minE/1290830‑1290957 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |