| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,292,843 | A→G | 33.9% | N105N (AAT→AAC) | yeeO ← | predicted multidrug efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,292,843 | 0 | A | G | 33.9% | 37.9 / 29.2 | 56 | N105N (AAT→AAC) | yeeO | predicted multidrug efflux system |
| Reads supporting (aligned to +/- strand): ref base A (12/25); new base G (7/12); total (19/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01 | |||||||||||
CATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCGCCAGAACAACACTTT > minE/1292777‑1292899 | cATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCAtt > 1:1340942/1‑69 (MQ=255) cGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATtctc < 1:3082106/69‑1 (MQ=255) cGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATtctc < 1:3395713/69‑1 (MQ=255) gCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATtctc > 1:3413127/1‑68 (MQ=255) gCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATtctc < 1:2039599/68‑1 (MQ=255) gCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATtctc < 1:2223871/68‑1 (MQ=255) gCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATtctc > 1:2845641/1‑68 (MQ=255) gCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATtctc < 1:3130664/68‑1 (MQ=255) gCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATtctc > 1:505939/1‑68 (MQ=255) gCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATtctc < 1:1414220/68‑1 (MQ=255) gCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATtctc < 1:688291/68‑1 (MQ=255) gCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATtctc > 1:1322292/1‑68 (MQ=255) gCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATtctc > 1:122370/1‑68 (MQ=255) gCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATtcta > 1:2761426/1‑67 (MQ=255) gCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTcc > 1:736969/1‑69 (MQ=255) cagcTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACa < 1:1290474/69‑1 (MQ=255) cagcTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACa < 1:1926693/69‑1 (MQ=255) cagcTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACa < 1:1946089/69‑1 (MQ=255) agcTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATtctc < 1:935427/52‑1 (MQ=255) gcTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGc < 1:1672170/69‑1 (MQ=255) gcTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGc < 1:2005163/69‑1 (MQ=255) gcTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGc < 1:1285985/69‑1 (MQ=255) gcTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGc < 1:723048/69‑1 (MQ=255) gcTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGc < 1:2375096/69‑1 (MQ=255) gcTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGc < 1:2931023/69‑1 (MQ=255) gcTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGc < 1:2887273/69‑1 (MQ=255) gcTGACCAGAAAAGCGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGc < 1:204171/69‑1 (MQ=255) ttaCCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATtctc < 1:2769238/47‑1 (MQ=255) ccAGAAAAGTGCTCAGGACCCCCATCAACAGGACACAGGCGTTCTCCATGAAGATAGGAACAGCAAGCg < 1:433646/69‑1 (MQ=255) aaaGTGCTCAGGACCCCCATCAACAGGACACAGGCGTTCTCCATGAAGATAGGAACAGCAAGCg < 1:2643761/64‑1 (MQ=255) aaaGTGCTCAGGACCCCCATCAACAGGACACAGGCGTTCTCCATGAAGATAGGAACAGCAAGCg < 1:3242847/64‑1 (MQ=255) aaaGTGCTCAGGACCCCCATCAACAGGACACAGGCGTTCTCCATGAAGATAGGAACAGCAAGCg < 1:3312380/64‑1 (MQ=255) aaaGTGCTCAGGACCCCCATCAACAGGACACAGGCGTTCTCCATGAAGATAGGAACAGCAAGCg < 1:1673703/64‑1 (MQ=255) aaaGTGCTCAGGACCCCCATCAACAGGACACAGGCGTTCTCCATGAAGATAGGAACAGCAAGCg < 1:3380068/64‑1 (MQ=255) aaaGTGCTCAGGACCCCCATCAACAGGACACAGGCGTTCTCCATGAAGATAGGAACAGCAAGCg < 1:153266/64‑1 (MQ=255) aaaGTGCTCAGGACCCCCATCAACAGGACACAGGCGTTCTCCATGAAGATAGGAACAGCAAGCg < 1:2769259/64‑1 (MQ=255) aaaGTGCTCAGGACCCCCATCAACAGGACACAGGCGTTCTCCATGAAGATAGGAACAGCAAGCg < 1:652061/64‑1 (MQ=255) aaaGTGCTCAGGACCCCCATCAACAGGACACAGGCGTTCTCCATGAAGATAGGAACAGCAAGCg < 1:2113885/64‑1 (MQ=255) aaaGTGCTCAGGACCCCCATCAACAGGACACAGGCGTTCTCCATGAAGATAGGAACAGCAAGCg < 1:2077922/64‑1 (MQ=255) aaaGTGCTCAGGACCCCCATCAACAGGACACAGGCGTTCTCCATGAAGATAGGAACAGCAAGCg < 1:2034303/64‑1 (MQ=255) aCCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCGCCAg < 1:1341148/69‑1 (MQ=255) cccccATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCGCCAGa < 1:2067688/69‑1 (MQ=255) ccccATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCGCCAGa < 1:2247676/68‑1 (MQ=255) cATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCGCCAGaacaa < 1:1013821/69‑1 (MQ=255) cATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCGCCAGaacaa < 1:2608332/69‑1 (MQ=255) aaCAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCGCCAGAACAacac > 1:2020347/1‑68 (MQ=255) aaCAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCGCCAGAACAACACt > 1:1687313/1‑69 (MQ=255) aaCAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCGCCAGAACAACACt > 1:1946391/1‑69 (MQ=255) aaCAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCGCCAGAACAACACt > 1:2252507/1‑69 (MQ=255) aGGCGTTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCGCCAGAACAACACttt > 1:3280502/1‑61 (MQ=255) aGGCGTTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCGCCAGAACAACACttt > 1:3200621/1‑61 (MQ=255) aGGCGTTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCGCCAGAACAACACttt > 1:3068264/1‑61 (MQ=255) aGGCGTTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCGCCAGAACAACACttt > 1:1561256/1‑61 (MQ=255) aGGCGTTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCGCCAGAACAACACttt > 1:1557224/1‑61 (MQ=255) aGGCGTTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCGCCAGAACAACACttt > 1:2982774/1‑61 (MQ=255) aGGCGTTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCGCCAGAACAACACttt > 1:234588/1‑61 (MQ=255) | CATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCGCCAGAACAACACTTT > minE/1292777‑1292899 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |