| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,293,306 | C→T | 68.1% | S307N (AGC→AAC) | cbl ← | DNA‑binding transcriptional activator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,293,306 | 0 | C | T | 68.1% | 37.8 / 23.3 | 50 | S307N (AGC→AAC) | cbl | DNA‑binding transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base C (10/6); new base T (23/11); total (33/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
ATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGT > minE/1293239‑1293359 | aTTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCt > 1:1011229/1‑69 (MQ=255)aTTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCt > 1:2047359/1‑69 (MQ=255) ttCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCtt < 1:1065319/69‑1 (MQ=255) ttCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCtt < 1:993813/69‑1 (MQ=255) aGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCtt < 1:920450/60‑1 (MQ=255) aGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCtt < 1:170342/60‑1 (MQ=255) aGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCtt < 1:562001/60‑1 (MQ=255) aGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCtt < 1:3368016/60‑1 (MQ=255) tCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCt > 1:1474115/1‑68 (MQ=255) tCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTg > 1:1442042/1‑69 (MQ=255) tCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTg > 1:763880/1‑69 (MQ=255) tCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTg > 1:199931/1‑69 (MQ=255) tGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGc > 1:3075710/1‑68 (MQ=255) tAATCAATTTCCTCTTCACTGTTTTCCATCACCTGGCGCTTGATATCCTCAACTGACAGTc < 1:734395/61‑1 (MQ=255) tAATCAATTTCCTCTTCACTGTTTTCCATCACCTGGCGCTTGATATCCTCAACTGACAGTc < 1:2385126/61‑1 (MQ=255) tAATCAATTTCCTCTTCACTGTTTTCCATCACCTGGCGCTTGATATCCTCAACTGACAGTc < 1:1242511/61‑1 (MQ=255) tAATCAATTTCCTCTTCACTGTTTTCCATCACCTGGCGCTTGATATCCTCAACTGACAGTc < 1:2882802/61‑1 (MQ=255) tAATCAATTTCCTCTTCACTGTTTTCCATCACCTGGCGCTTGATATCCTCAACTGACAGTc < 1:3253787/61‑1 (MQ=255) tAATCAATTTCCTCTTCACTGTTTTCCATCACCTGGCGCTTGATATCCTCAACTGACAGTc < 1:2662745/61‑1 (MQ=255) tAATCAATTTCCTCTTCACTGTTTTCCATCACCTGGCGCTTGATATCCTCAACTGACAGTc < 1:838536/61‑1 (MQ=255) tAATCAATTTCCTCTTCACTGTTTTCCATCACCTGGCGCTTGATATCCTCAACTGACAGTc < 1:1212435/61‑1 (MQ=255) tAATCAATTTCCTCTTCACTGTTTTCCATCACCTGGCGCTTGATATCCTCAACTGACAGTc < 1:1717882/61‑1 (MQ=255) tAATCAATTTCCTCTTCACTGTTTTCCATCACCTGGCGCTTGATATCCTCAACTGACAGTc < 1:2138765/61‑1 (MQ=255) tAATCAATTTCCTCTTCACTGTTTTCCATCACCTGGCGCTTGATATCCTCAACTGACAGTc < 1:1551131/61‑1 (MQ=255) tactctTCACTGCTTTCCATCACCTGCCGCTTGATATCCTc > 1:2726557/3‑41 (MQ=255) tCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGt > 1:1468813/1‑66 (MQ=255) tCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGt > 1:2827880/1‑66 (MQ=255) cTGTTTTCCATCACCTGGCGCTTGATATCCTCAACTGACAGTCCTGCATTAc > 1:2304063/1‑52 (MQ=39) cTGTTTTCCATCACCTGGCGCTTGATATCCTCAACTGACAGTCCTGCATTAc > 1:89404/1‑52 (MQ=39) cTGTTTTCCATCACCTGGCGCTTGATATCCTCAACTGACAGTCCTGCATTACa > 1:1685529/1‑53 (MQ=39) cTGTTTTCCATCACCTGGCGCTTGATATCCTCAACTGACAGTCCTGCATTACa > 1:805333/1‑53 (MQ=39) cTGTTTTCCATCACCTGGCGCTTGATATCCTCAACTGACAGTCCTGCATTACa > 1:2277630/1‑53 (MQ=39) cTGTTTTCCATCACCTGGCGCTTGATATCCTCAACTGACAGTCCTGCATTACa > 1:1398729/1‑53 (MQ=39) cTGTTTTCCATCACCTGGCGCTTGATATCCTCAACTGACAGTCCTGCATTACa > 1:721442/1‑53 (MQ=39) cTGTTTTCCATCACCTGGCGCTTGATATCCTCAACTGACAGTCCTGCATTACa > 1:715427/1‑53 (MQ=39) cTGTTTTCCATCACCTGGCGCTTGATATCCTCAACTGACAGTCCTGCATTACa > 1:667560/1‑53 (MQ=39) cTGTTTTCCATCACCTGGCGCTTGATATCCTCAACTGACAGTCCTGCATTACa > 1:597326/1‑53 (MQ=39) cTGTTTTCCATCACCTGGCGCTTGATATCCTCAACTGACAGTCCTGCATTACa > 1:1797702/1‑53 (MQ=39) cTGTTTTCCATCACCTGGCGCTTGATATCCTCAACTGACAGTCCTGCATTACa > 1:1240379/1‑53 (MQ=39) cTGTTTTCCATCACCTGGCGCTTGATATCCTCAACTGACAGTCCTGCATTACa > 1:2074863/1‑53 (MQ=39) cTGTTTTCCATCACCTGGCGCTTGATATCCTCAACTGACAGTCCTGCATTACa > 1:3194037/1‑53 (MQ=39) cTGTTTTCCATCACCTGGCGCTTGATATCCTCAACTGACAGTCCTGCATTACa > 1:2093034/1‑53 (MQ=39) cTGTTTTCCATCACCTGGCGCTTGATATCCTCAACTGACAGTCCTGCATTACa > 1:2689226/1‑53 (MQ=39) cTGTTTTCCATCACCTGGCGCTTGATATCCTCAACTGACAGTCCTGCATTACa > 1:2218375/1‑53 (MQ=39) cTGTTTTCCATCACCTGGCGCTTGATATCCTCAACTGACAGTCCTGCATTACa > 1:2423296/1‑53 (MQ=39) cTGTTTTCCATCACCTGGCGCTTGATATCCTCAACTGACAGTCCTGCATTACa > 1:2408775/1‑53 (MQ=39) cTGTTTTCCATCACCTGGCGCTTGATATCCTCAACTGACAGTCCTGCATTACa > 1:2253072/1‑53 (MQ=39) cTGTTTTCCATCACCTGGCGCTTGATATCCTCAACTGACAGTCCTGCATTACa > 1:233913/1‑53 (MQ=39) cTGTTTTCCATCACCTGGCGCTTGATATCCTCAACTGACAGTCCTGCATGACa > 1:3071955/1‑53 (MQ=38) cTGTTTTCCATCACCTGGCGCTGGATATCCTCAACTGACAGTCCTGCATTACa > 1:3122992/1‑53 (MQ=38) | ATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGT > minE/1293239‑1293359 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |