| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,293,839 | C→A | 44.8% | G129G (GGG→GGT) | cbl ← | DNA‑binding transcriptional activator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,293,839 | 0 | C | A | 44.8% | 24.0 / 53.7 | 58 | G129G (GGG→GGT) | cbl | DNA‑binding transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base C (13/19); new base A (16/10); total (29/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.72e-01 | |||||||||||
ACGCTCGCTGGCGATACCAATATCAGCTTCGCCATTTTGCAACAATGTCGCAATTTCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGACCTC > minE/1293773‑1293898 | aCGCTCGCTGGCGATACCAATATCAGCTTCGCCATTTTGCAACAATGTCGCAATTTCCTGTGGCGTc < 1:1259942/67‑1 (MQ=255)aCGCTCGCTGGCGATACCAATATCAGCTTCGCCATTTTGCAACAATGTCGCAATTTCCTGTGGCGTc < 1:3339827/67‑1 (MQ=255) acgaaTATCAGCTTCGCCATTTTGCAACAATGTCGCAATTTCCTGTGGCGTCCCCTGGATTAGCTCGAg > 1:720523/4‑69 (MQ=255) aCCAATATCAGCTTCGCCATTTTGCAACAATGTCGCAATTTCCTTTGGCGTCCCCTGGATTAGCTCGAg > 1:2608954/1‑69 (MQ=255) aCCAATATCAGCTTCGCCATTTTGCAACAATGTCGCAATTTCCTGTGGCGTCCCCTGGATTAGCTCgg > 1:3281865/1‑67 (MQ=255) aCCAATATCAGCTTCGCCATTTTGCAACAATGTCGCAATTTCCTGTGGCGTCCCCTGGATTAGCTCGAg > 1:3037754/1‑69 (MQ=255) aCCAATATCAGCTTCGCCATTTTGCAACAATGTCGCAATTTCCTGTGGCGTCCCCTGGATTAGCTCGAg > 1:3108470/1‑69 (MQ=255) aCCAATATCAGCTTCGCCATTTTGCAACAATGTCGCAATTTCCTGTGGCGTCCCCTGGATTAGCTCGAg > 1:3434523/1‑69 (MQ=255) aCCAATATCAGCTTCGCCATTTTGCAACAATGTCGCAATTTCCTGTGGCGTCCCCTGGATTAGCTCGAg > 1:2258749/1‑69 (MQ=255) atatCAGCTTCGCCATTTTGCAACAATGTCGCAATTTCCTGTGGCGTCCCCTGGATTAGCTCGAGCCgt < 1:3346705/69‑2 (MQ=255) atatCAGCTTCGCCATTTTGCAACAATGTCGCAATTTCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGa < 1:2216290/69‑1 (MQ=255) atatCAGCTTCGCCATTTTGCAACAATGTCGCAATTTCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGa < 1:1956732/69‑1 (MQ=255) atatCAGCTTCGCCATTTTGCAACAATGTCGCAATTTCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGa < 1:1881122/69‑1 (MQ=255) atatCAGCTTCGCCATTTTGCAACAATGTCGCAATTTCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGa < 1:3382019/69‑1 (MQ=255) atatCAGCTTCGCCATTTTGCAACAATGTCGCAATTTCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGa < 1:1417802/69‑1 (MQ=255) atatCAGCTTCGCCATTTTGCAACAATGTCGCAATTTCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGa < 1:2461205/69‑1 (MQ=255) aGCTTCGCCATTTTGCAACAATGTCGCAATTTCCTGTGGCGTCCCCTgg < 1:2517724/49‑1 (MQ=255) cATTTTGCAACAATGTCGCAATTTCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGaaa < 1:577810/68‑1 (MQ=255) aTTTTGCAACAATGTCGCAATTTCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAg < 1:2475816/69‑1 (MQ=255) aTTTTGCAACAATGTCGCAATTTCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAg < 1:435476/69‑1 (MQ=255) tttGCAACAACGTCGCAATTTCCTGTGGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGa > 1:320809/1‑63 (MQ=255) tttGCAACAACGTCGCAATTTCCTGTGGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGa > 1:3243634/1‑63 (MQ=255) tttGCAACAACGTCGCAATTTCCTGTGGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGa > 1:3299173/1‑63 (MQ=255) tttGCAACAACGTCGCAATTTCCTGTGGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGa > 1:331489/1‑63 (MQ=255) tttGCAACAACGTCGCAATTTCCTGTGGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGa > 1:3034404/1‑63 (MQ=255) tttGCAACAACGTCGCAATTTCCTGTGGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGa > 1:2716171/1‑63 (MQ=255) tttGCAACAACGTCGCAATTTCCTGTGGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGa > 1:467437/1‑63 (MQ=255) tttGCAACAACGTCGCAATTTCCTGTGGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGa > 1:712178/1‑63 (MQ=255) tttGCAACAACGTCGCAATTTCCTGTGGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGa > 1:738839/1‑63 (MQ=255) tttGCAACAACGTCGCAATTTCCTGTGGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGa > 1:868437/1‑63 (MQ=255) tttGCAACAACGTCGCAATTTCCTGTGGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGa > 1:1675227/1‑63 (MQ=255) tttGCAACAACGTCGCAATTTCCTGTGGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGa > 1:1762984/1‑63 (MQ=255) tttGCAACAACGTCGCAATTTCCTGTGGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGa > 1:1823508/1‑63 (MQ=255) tttGCAACAACGTCGCAATTTCCTGTGGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGa > 1:2411853/1‑63 (MQ=255) tttGCAACAACGTCGCAATTTCCTGTGGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGa > 1:2190662/1‑63 (MQ=255) tttGCAACAACGTCGCAATTTCCTGTGGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGa > 1:2226343/1‑63 (MQ=255) tGCAACAATGTCGCAATTTCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAg < 1:124799/65‑1 (MQ=255) gCAATTTCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCttt < 1:537777/68‑1 (MQ=255) aaTTTCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAAt < 1:2463215/69‑1 (MQ=255) aaTTTCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAAt < 1:620338/69‑1 (MQ=255) tCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGAcc < 1:2302878/69‑1 (MQ=255) tCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGAcc > 1:1977045/1‑69 (MQ=255) tCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGAcc < 1:1042751/69‑1 (MQ=255) tCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGAcc > 1:2432338/1‑69 (MQ=255) tCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGAcc > 1:1261594/1‑69 (MQ=255) tCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGAcc < 1:2677240/69‑1 (MQ=255) tCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGAcc > 1:1734634/1‑69 (MQ=255) tCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGAcc > 1:1493766/1‑69 (MQ=255) tCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGAcc > 1:14402/1‑69 (MQ=255) tCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGAcc < 1:353439/69‑1 (MQ=255) tgtgGTGTACCCTTGATTAGCTCCAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTATTGACctc < 1:1035155/68‑1 (MQ=255) tgtgGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGACctc < 1:478415/68‑1 (MQ=255) tgtgGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGACctc < 1:1336575/68‑1 (MQ=255) tgtgGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGACctc < 1:1328644/68‑1 (MQ=255) tgtgGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGACctc < 1:1387458/68‑1 (MQ=255) tgtgGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGACctc < 1:3270729/68‑1 (MQ=255) tgtgGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGACctc < 1:785902/68‑1 (MQ=255) tgtgGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGACctc < 1:2436497/68‑1 (MQ=255) tgtgGTGTACCCTGGATTAGCTCCAGCCGAAACTCCGGGAAAAGTTCGCTAAAAGCTTTAATGACctc < 1:1795364/68‑1 (MQ=255) tgtgGTGTACCCTGGATTAGCTCCACCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGACctc < 1:2842121/68‑1 (MQ=255) | ACGCTCGCTGGCGATACCAATATCAGCTTCGCCATTTTGCAACAATGTCGCAATTTCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGACCTC > minE/1293773‑1293898 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |