| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 104,896 | T→C | 58.1% | intergenic (+21/+22) | ampE → / ← aroP | predicted inner membrane protein/aromatic amino acid transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 104,896 | 0 | T | C | 58.1% | 7.4 / 18.8 | 31 | intergenic (+21/+22) | ampE/aroP | predicted inner membrane protein/aromatic amino acid transporter |
| Reads supporting (aligned to +/- strand): ref base T (10/3); new base C (5/13); total (15/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.13e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGG > minE/104842‑104943 | tGCGCTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTTCACggg > 1:825378/1‑60 (MQ=255)tGCGCTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTTCACGGGTGAggg > 1:1354653/1‑66 (MQ=255)tGCGCTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTTCACGGGTGAggg > 1:2697976/1‑66 (MQ=255)tGCGCTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTTCACGGGTGAggg > 1:3278156/1‑66 (MQ=255)tGCGCTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTTCACGGGTGAggg > 1:2460488/1‑66 (MQ=255) ctGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACTCTGTACGGGTGAGGGCGTagagag > 1:889941/1‑68 (MQ=255) ctGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACTCTGTACGGGTGAGGGCGTagagag > 1:2756731/1‑68 (MQ=255) ctGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACTCTGTACGGGTGAGGGCGTagagag > 1:2998692/1‑68 (MQ=255) cGATTTACGGGGCGTTGGTGTAAAGATTATTGCCTTCACCCTGCACGGGTg < 1:223844/51‑1 (MQ=255) cGATTTACGGGGCGTTGGTGTAAAGATTATTGCCTTCACCCTGCACGGGTg < 1:2997897/51‑1 (MQ=255) cGATTTACGGGGCGTTGGTGTAAAGATTATTGCCTTCACCCTGCACGGGTg < 1:2780184/51‑1 (MQ=255) cGATTTACGGGGCGTTGGTGTAAAGATTATTGCCTTCACCCTGCACGGGTg < 1:2671544/51‑1 (MQ=255) cGATTTACGGGGCGTTGGTGTAAAGATTATTGCCTTCACCCTGCACGGGTg < 1:2649552/51‑1 (MQ=255) cGATTTACGGGGCGTTGGTGTAAAGATTATTGCCTTCACCCTGCACGGGTg < 1:2532665/51‑1 (MQ=255) cGATTTACGGGGCGTTGGTGTAAAGATTATTGCCTTCACCCTGCACGGGTg < 1:1637871/51‑1 (MQ=255) cGATTTACGGGGCGTTGGTGTAAAGATTATTGCCTTCACCCTGCACGGGTg < 1:835633/51‑1 (MQ=255) cGATTTACGGGGCGTTGGTGTAAAGATTATTGCCTTCACCCTGCACGGGTg < 1:1402308/51‑1 (MQ=255) cGATTTACGGGGCGTTGGTGTAAAGATTATTGCCTTCACCCTGCACGGGTg < 1:1202343/51‑1 (MQ=255) cGATTTACGGGGCGTTGGTGTAAAGATTATTGCCTTCACCCTGCACGGGTg < 1:926849/51‑1 (MQ=255) gATTTACGGGGCGTTGGTGTAAAGATTATTGCCTTCACCCTGCACGGGTg < 1:852880/50‑1 (MQ=255) tGGTGTAAAGATTATTGCCTTCACCCTGCACGGGTg < 1:328982/36‑1 (MQ=38) ggTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATg < 1:1161999/55‑1 (MQ=255) tAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATg < 1:1267552/51‑1 (MQ=255) aaaGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATg < 1:1086282/50‑1 (MQ=255) aaGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTaga > 1:1361568/1‑39 (MQ=255) aaGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTa > 1:2049100/1‑37 (MQ=255) aaGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTa > 1:2328444/1‑57 (MQ=255) aaGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCgg > 1:2559605/1‑69 (MQ=255) aaGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCgg > 1:3266590/1‑69 (MQ=255) aaGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCgg > 1:184176/1‑69 (MQ=255) aaGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCgg > 1:1575029/1‑69 (MQ=255) | TGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGG > minE/104842‑104943 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |