| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,294,417 | T→C | 36.4% | L276L (TTA→TTG) | nac ← | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,294,417 | 0 | T | C | 36.4% | 50.0 / 52.3 | 85 | L276L (TTA→TTG) | nac | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base T (21/33); new base C (16/15); total (37/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01 | |||||||||||
TTTTTTCCATCACTGGAGAACTAATCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGT > minE/1294350‑1294480 | ttttttCCATCACTGGAGAACTAATCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGAt < 1:2138076/68‑1 (MQ=255)ttttttCCATCACTGGAGAACTAATCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGAt < 1:260809/68‑1 (MQ=255) aCTGGAGAACTAATCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTg > 1:471948/1‑69 (MQ=255) aCTGGAGAACTAATCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTg > 1:1374557/1‑69 (MQ=255) aaTCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCg > 1:1345410/1‑61 (MQ=255) aaTCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCg > 1:582460/1‑61 (MQ=255) aaTCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTaaa > 1:1887239/1‑67 (MQ=255) aaTCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTaaa > 1:3240263/1‑67 (MQ=255) aaTCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTaaa > 1:143635/1‑67 (MQ=255) aaTCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTaaa > 1:1985688/1‑67 (MQ=255) aaTCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTAAAt > 1:1056387/1‑68 (MQ=255) aaTCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTAAAt > 1:3401758/1‑68 (MQ=255) aaTCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTAAAt > 1:2576803/1‑68 (MQ=255) aaTCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTAAAt > 1:1749897/1‑68 (MQ=255) aaTCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTAAAt > 1:765992/1‑68 (MQ=255) aaTCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTAAAt > 1:1141716/1‑68 (MQ=255) aaTCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTAAAt > 1:2362576/1‑68 (MQ=255) aaTCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTAAAt > 1:3383063/1‑68 (MQ=255) aaTCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTAAAt > 1:1359146/1‑68 (MQ=255) aaTCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTAAAt > 1:2200556/1‑68 (MQ=255) cACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTAAAttt < 1:2557345/67‑1 (MQ=255) cACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTAAAttt < 1:643392/67‑1 (MQ=255) cACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTAAAttt < 1:2618313/67‑1 (MQ=255) cACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTAAAttt < 1:350005/67‑1 (MQ=255) cACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTAAAttt < 1:470355/67‑1 (MQ=255) cACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTAAAttt < 1:427821/67‑1 (MQ=255) cACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTAAAttt < 1:403133/67‑1 (MQ=255) cACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTAAAttt < 1:372293/67‑1 (MQ=255) cACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTAAAttt < 1:2006242/67‑1 (MQ=255) cACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTAAAttt < 1:844807/67‑1 (MQ=255) cACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTAAAttt < 1:2002352/67‑1 (MQ=255) cACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTAAAttt < 1:1614331/67‑1 (MQ=255) cACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTAAAttt < 1:1983008/67‑1 (MQ=255) cACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTAAAttt < 1:1831097/67‑1 (MQ=255) cACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAAttt < 1:94592/67‑1 (MQ=255) cACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAAttt < 1:3297362/67‑1 (MQ=255) cACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAAttt < 1:450242/67‑1 (MQ=255) cACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAAttt < 1:1662947/67‑1 (MQ=255) cACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAAttt < 1:1021220/67‑1 (MQ=255) cACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGAGGGTAAAttt < 1:2506822/67‑1 (MQ=255) cACTGACATTAACAACTCTTTCACTGCCAGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAAttt < 1:1141574/67‑1 (MQ=255) aCTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAAt > 1:2229725/1‑69 (MQ=255) aCTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAAt > 1:3407443/1‑69 (MQ=255) aCTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAAt > 1:2286988/1‑69 (MQ=255) tGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTAAAttt < 1:1301192/64‑1 (MQ=255) aTTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACaaa < 1:795354/69‑1 (MQ=255) aTTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACaaa < 1:844453/69‑1 (MQ=255) aTTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACaaa < 1:748245/69‑1 (MQ=255) aTTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACaaa < 1:371605/69‑1 (MQ=255) aTTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACaaa < 1:3376550/69‑1 (MQ=255) aTTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACa < 1:2574273/67‑1 (MQ=255) aTTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCCGGTAAATTTAATGACaaa < 1:2236309/69‑1 (MQ=255) tAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAaga < 1:1108862/69‑1 (MQ=255) aacaacTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACaaa > 1:1751982/1‑66 (MQ=255) aacaacTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACaaa > 1:2384591/1‑66 (MQ=255) aacaacTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACaaa > 1:3302679/1‑66 (MQ=255) aacaacTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACaaa > 1:755710/1‑66 (MQ=255) ttCACTGCCTGTGCCTGTGGCGATAAGTTCGctct > 1:2425734/1‑35 (MQ=255) ttCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCAt > 1:2832004/1‑68 (MQ=255) ttCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCAt > 1:860750/1‑68 (MQ=255) ttCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCAt > 1:649082/1‑68 (MQ=255) ttCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCAt > 1:2110337/1‑68 (MQ=255) ttCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCAt > 1:2587328/1‑68 (MQ=255) ttCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCAt > 1:32009/1‑68 (MQ=255) ttCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCAt > 1:3182513/1‑68 (MQ=255) ttCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCAt > 1:1377795/1‑68 (MQ=255) ttCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCAt > 1:2976663/1‑68 (MQ=255) tgcctgtgcctgTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACt < 1:3338923/60‑1 (MQ=255) tgcctgtgcctgTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAgg < 1:490750/69‑1 (MQ=255) tgcctgtgcctgTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAgg < 1:2934565/69‑1 (MQ=255) tgcctgtgcctgTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAgg < 1:873604/69‑1 (MQ=255) tgcctgtgcctgTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAgg < 1:1480796/69‑1 (MQ=255) tgcctgtgcctgTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAgg < 1:2845626/69‑1 (MQ=255) tgcctgtgcctgTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAgg < 1:1141889/69‑1 (MQ=255) tgcctgtgcctgTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAgg < 1:837029/69‑1 (MQ=255) tgcctgtgcctgTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAgg < 1:1726422/69‑1 (MQ=255) tgcctgtgcctgTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAgg < 1:782788/69‑1 (MQ=255) tgcctgtgcctgTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAgg < 1:1655149/69‑1 (MQ=255) gcctgtgcctgtGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAgg < 1:965572/68‑1 (MQ=255) cctgtgGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAaga < 1:586339/46‑1 (MQ=255) cctgtgGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAaga < 1:583241/46‑1 (MQ=255) cctgtgGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAaga < 1:873590/46‑1 (MQ=255) cctgtgGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAaga < 1:3408701/46‑1 (MQ=255) cctgtgGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGt < 1:3282400/67‑1 (MQ=255) gCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGt > 1:2555794/1‑61 (MQ=255) gCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGt > 1:3127008/1‑61 (MQ=255) gCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGt < 1:1328243/68‑1 (MQ=255) | TTTTTTCCATCACTGGAGAACTAATCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGT > minE/1294350‑1294480 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |